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Efficient Decolorization of Dye Acid Blue 113 by Soil Bacterium Bacillus subtilis RMLP2


Affiliations
1 Department of Biochemistry, Dr. Rammanohar Lohia Avadh University, Ayodhya – 224001, Uttar Pradesh, India
2 Department of Biotechnology, Guru Ghasidas University, Koni, Bilaspur – 495009, Chhattisgarh, India
3 Department of Biotechnology, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh, India
     

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In this study, a bacterial strain was isolated from soil and tested for the decolorization of azo dye Acid Blue 113 (AB 113). Decolorization of azo dyes by means of physico-chemical method is not environmentally friendly thus an alternative method based on bacteria was employed for decolorization of AB 113. The color removal studies were performed using Bushnell and Hass medium amended with AB 113 dye. Bacterial isolate Bacillus subtilis RMLP2 was identified by 16S rRNA gene sequence analysis. The effect of various physico-chemical parameters such as incubation condition, pH, temperature, carbon source, nitrogen source and dye concentration on decolorization of AB 113 by Bacillus subtilis RMLP2 were studied. The bacterial isolate showed the remarkable higher percentage (92.71%) of color removal of dye AB 113 at 100 mg/L concentration, 35oC, pH 7 during 72 h of incubation period under static condition. Yeast extract and glucose was found as best nitrogen and carbon source for efficient decolorization of dye. These results confirmed that the Bacillus subtilis has enormous ability to degrade dye AB 113 present in textile effluents.

Keywords

Acid Blue 113, Azo Dye, Bacillus subtilis, Decolorization, 16S rDNA.
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  • Efficient Decolorization of Dye Acid Blue 113 by Soil Bacterium Bacillus subtilis RMLP2

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Authors

Pradeep Kumar Singh
Department of Biochemistry, Dr. Rammanohar Lohia Avadh University, Ayodhya – 224001, Uttar Pradesh, India
Rajat Pratap Singh
Department of Biotechnology, Guru Ghasidas University, Koni, Bilaspur – 495009, Chhattisgarh, India
Pankaj Singh
Department of Biotechnology, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh, India
Ram Lakhan Singh
Department of Biochemistry, Dr. Rammanohar Lohia Avadh University, Ayodhya – 224001, Uttar Pradesh, India

Abstract


In this study, a bacterial strain was isolated from soil and tested for the decolorization of azo dye Acid Blue 113 (AB 113). Decolorization of azo dyes by means of physico-chemical method is not environmentally friendly thus an alternative method based on bacteria was employed for decolorization of AB 113. The color removal studies were performed using Bushnell and Hass medium amended with AB 113 dye. Bacterial isolate Bacillus subtilis RMLP2 was identified by 16S rRNA gene sequence analysis. The effect of various physico-chemical parameters such as incubation condition, pH, temperature, carbon source, nitrogen source and dye concentration on decolorization of AB 113 by Bacillus subtilis RMLP2 were studied. The bacterial isolate showed the remarkable higher percentage (92.71%) of color removal of dye AB 113 at 100 mg/L concentration, 35oC, pH 7 during 72 h of incubation period under static condition. Yeast extract and glucose was found as best nitrogen and carbon source for efficient decolorization of dye. These results confirmed that the Bacillus subtilis has enormous ability to degrade dye AB 113 present in textile effluents.

Keywords


Acid Blue 113, Azo Dye, Bacillus subtilis, Decolorization, 16S rDNA.

References





DOI: https://doi.org/10.18311/ti%2F2021%2Fv28i3%2F27736