Toxicology International (Formerly Indian Journal of Toxicology)

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Chronic Toxicity of Indoxacarb and its Amelioration by Rhizome Powder of Hedychium spicatumin WLH Cockerels


Affiliations
1 ICAR-KVK, IIVR, Bejwa, Bhadohi, U.P., India
2 Department of Veterinary Pharmacology & Toxicology, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, India
     

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The objective of the present study was to evaluate the chronic toxicity by determining biochemical parameters after prolong exposure with indoxacarb @ 250 ppm and its amelioration by concurrent administration of rhizome powder of Hydechiumspicatum (HSRP) @ 2000ppm and 4000ppm in feed for 16 weeks in WLH cockerels. The total protein, globulins, albumins and glucose levels in blood serum were significantly (P<0.05) decreased whereas direct bilirubin, indirect bilirubin, BUN, creatinine, ALT, AST, ALP, GGT were significantly (P<0.05) increased in indoxacarb intoxicated cockerels groups indicating hepatotoxic and nephrotoxic effects. Simultaneous administration of HSRP in both 2000ppm and 4000pp min diet produced amelioration in biochemical profile of indoxacarb intoxicated cockerels. Thus, it is concluded that HSRP was effective in restoration of biochemical parameters and revealed hepatoprotective and nephroprotective effects in 16 weeks feeding trial in cockerels.

Keywords

Biochemical, Indoxacarb, Hedychium spicatum, WLH Cockerels.
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  • ThapliyalS, Juyal V, Bhandari A. Hepatoprotective and Antioxidant Activity of Methanol Extract of Hedychium spicatum against CCl4-Induced Liver Injury in Rats. Res J Pharmaceutical, Biol. and Chem. Sc.; 2014; 5 (2): 1428.

  • Puri A, Srivastava A, Bhardwaj A, Tondon J S, Saxena K C. Journal of Medicinal Plants Research; 2013, 7 (44): 3242-3246.

  • Kajaria D, Tripathi J S, Tiwari S K, Pandey B L. In-vitro evaluation of immunomodulatory effect of polyherbal compound – Bharangyadi. Journal of Drug Delivery & Therap.; 2013, 3 (1): 36-39.

  • Choudhary G K, Singh S P. Cytotoxic potential of rhizome extracts of Hedychium spicatum L. in HepG2 cell line using MTT, Indian J of Animal Sc.; 2017, 87 (3): 313–315.

  • Singh K L, Bagh G C. Phytochemical Analysis and Determination of Total Phenolics Content In Water Extracts of Three Species of Hedychium. Intern J. Pharm. Tech. Res.; CODEN (USA),;2013, 5 (4): 1516-1521.

  • Choudhary G K, Singh S P. (2017). Ameliorating potential of Hedychium spicatum on oxidative stress following chronic exposure to indoxacarb in WLH cockerels, Indian J Animal Res; 2017, 51 (5):

  • Sravani T, Paarakh P M. Antioxidant property of Hedychium spicatum Buch.-Ham. rhizomes, Ind. J. of Natural Product and Resources; 2012, 3 (3): 354-358.

  • Thapliyal S, Juya V, Bhandari A. Pharmacognostic Screening of Hedychium spicatum Rhizomes, Inter J of Pharmaceutical and Chem Sc.; 2014, 3 (4): 483-488.

  • Choudhary G K, Singh S P. Ameliorative efficacy of Hedychium spicatum ischolar_main powder in indoxacarb intoxicated WLH cockerels. J. Vet. Parmacol. & Toxicol.; 2016, 15 (2): 61-64.

  • Lapied B, Grolleau F, Sattelle D B. Indoxacarb, an oxadiazine insecticide, blocks insect neuronal sodium channels, British J Pharmacol.; 2001, 132: 587−595.

  • Mudaraddi T Y, Kaliwal B B. Indoxacarb induces testes oxidative stress in Swiss Albino Mice. Inter J Biotech. Res.; 2009, 1: 40-48.

  • Mudaraddi T Y, Potadar R R, Kaliwal B B. Indoxacarb induces liver oxidative stress in Swiss Albino Mice, Eur J of Experi Bio.; 2012, 2 (1): 180-186.

  • Frame S R. Oncogenicity study with DPX-JW062-106 (50% DPX-KN128, 50% DPX-KN127) eighteen-month feeding study in mice. Unpublished report No. HLR 799-96 from DuPont Haskell Laboratory, Newark, Delaware, USA. 1997, Submitted to WHO by E.I. du Pont de Nemours and Company, Wilmington, Delaware, USA.

  • Mertens J J W M. (1997). Subchronic oral toxicity: 90-day study with DPX-JW062-106 (50% DPX-KN128, 50% DPXKN127) feeding study in dogs. Unpublished report No. HLO 494-95, RV3 from WIL Research Laboratories, Inc., Ashland, Ohio, USA. 1997. Submitted to WHO by E.I. du Pont de Nemours and Company, Wilmington, Delaware, USA.

  • Wolf P L, Williams D, Coplon N, Coulson A S. Low aspartate transminase activity in serum of patients undergoing chonichaemolysis. Clin. Chem.; 1972.18:567.

  • Moss D W, Henderson A K. Clinical enzymology, In: Tietz Textbook of clinical chemistry, 3rd ed., C. A. Burtis and E. R. Ashwood, Eds. W.B. Saunders, Philadelphia. 1994, pp 617-721.

  • Young D S. Effects of drugs on clinical Laboratory tests. Third Edition, AACC Press, 1997, 937 pp.

  • Bower L D, Wong E T. Kinetic Serum Creatinine Assays. II. A Critical Evaluation and review, Clin. Chem.; 1980, 26(5):555-561.

  • Lowry O H, Rosebrough N J, Farr A L, Randall R J. 1951. Total protein estimation by lowery’s Methods, J. Biol. Chem; 1951, 193: 265.

  • Snedecor G W, Cochran W G. Statistical methods, 9th edition. Iowa State University Press, 1994, Ame.

  • Malek D E. Subchronic oral toxicity: 90-day study with DPX-JW062 (50% DPX-KN128, 50% DPX-KN127) feeding study in rats. Unpublished report No. HLR 751-93, RV2 from DuPont Haskell Laboratory, Newark, Delaware, USA; 1997, Submitted to WHO by E.I. du Pont de Nemours and Company, Wilmington, Delaware, USA.

  • Robinson M W, Harmon C, O’Farrelly C. Liver immunology and its role in inflammation and homeostasis. Cellular and Mol Immunol.; 2016, 13: 267-276.

  • Joshi U, Mishra S H. Preliminary evaluation of immunomodulatory and antistress activity of methanol extract of Hedychium spicatum. Pharmacologyonline, 2009, 1: 1057-1071.

  • Westwood A. The analysis of bilirubin in serum. Annals of clinical Biochemistry. 1991, 28 (2): 119.

  • Benzamin M M. Outline of Veterinary clinical pathology, ed 3rd Kalyani Publishers, New Delhi. 2010, pp 241-245.

  • Guyton A C, Hall J E. Text book of medical physiology, 11th edn. Elsevier saunder, Philadelphia, 2006, pp. 343-344.

  • Rosalki S B, Mcintyre N. Biochemical investigations in the management of liver disease. Oxford textbook of clinical hepatology, 2nd ed. New York, Oxford University Press, 1999, pp. 503-521.

  • Dufour D R, Lott J A, Nolte F S, Gretch D R, Koff R S. Diagnosis and monitoring of hepatic injury. II. Recommendations for use of laboratory tests in screening, diagnosis and monitoring. Clin. Chem.; 2001, 47: 1133-1135.

  • Amacher D E. A toxicologist’s guide to biomarkers of hepatic response. Hum. Exp. Toxicol.; 2002. 21: 253-262.

  • Goyal S, Sandhu H S. Toxic effects of sub-chronic oral exposure of indoxacarb on biochemical parameters in buffalo calves, Toxicol. Int.; 2009, 16 (2): 141-146.

  • Goyal S, Sandhu H S. Clinicobiochemical evaluation of Sub-acute toxicity of indoxacarb in buffalo calves. J. Vety. Pharmacol. & Toxicol.; 2014, 13 (1): 63-66.

  • Kumar A, Garg U K, Gupta S K, Jatav G P, Rajput Neetu. Effect of indoxacarb induced toxicity on biochemical parameters in broilers (Gallus domesticua). J Vety. Pharmacol. & Toxicol.; 2014, 12 (1-2) : 21-23.

  • Rosen H R, Keefe E B. Evaluation of abnormal liver enzymes, use of liver tests and the serology of viral hepatitis: Liver disease, diagnosis and management. 1st edn. New York, Churchill Livingstone Publishers, 2000, pp. 24-35.

  • Friedman, S. F., Martin, P. and Munoz, J. S. Laboratory evaluation of the patient with liver disease.Hepatology, a text-book of liver disease.Philedelphia, Saunders Publication, 1 : 661-709.

  • Filip R, Lopez P, Giberti G, Coussio J, Ferraro G. Phenolic compounds in seven south American Ilex species. ‘Fitoterapia’ 2001, 72 (7): 774-778.

  • Thapa B R, Walia A. Liver function test and their interpretation. Indian J. Pediatr.; 2007, 74: 663-671.

  • Gordon T. Factors associated with serum alkaline phosphatase level. Arch. Pathol. Lab. Med., 1993; 117 : 187-193.

  • Sibal A, Gopalan S. 2015. Text book of pediatric gastroenterology, hepatology and nutritions. 1st edn.,Jaypee Brothers Medical Publications (P) Ltd. New Delhi, 2015; pp. 301-302.


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  • Chronic Toxicity of Indoxacarb and its Amelioration by Rhizome Powder of Hedychium spicatumin WLH Cockerels

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Authors

Govind Kumar Choudhary
ICAR-KVK, IIVR, Bejwa, Bhadohi, U.P., India
Satya Pal Singh
Department of Veterinary Pharmacology & Toxicology, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, India

Abstract


The objective of the present study was to evaluate the chronic toxicity by determining biochemical parameters after prolong exposure with indoxacarb @ 250 ppm and its amelioration by concurrent administration of rhizome powder of Hydechiumspicatum (HSRP) @ 2000ppm and 4000ppm in feed for 16 weeks in WLH cockerels. The total protein, globulins, albumins and glucose levels in blood serum were significantly (P<0.05) decreased whereas direct bilirubin, indirect bilirubin, BUN, creatinine, ALT, AST, ALP, GGT were significantly (P<0.05) increased in indoxacarb intoxicated cockerels groups indicating hepatotoxic and nephrotoxic effects. Simultaneous administration of HSRP in both 2000ppm and 4000pp min diet produced amelioration in biochemical profile of indoxacarb intoxicated cockerels. Thus, it is concluded that HSRP was effective in restoration of biochemical parameters and revealed hepatoprotective and nephroprotective effects in 16 weeks feeding trial in cockerels.

Keywords


Biochemical, Indoxacarb, Hedychium spicatum, WLH Cockerels.

References





DOI: https://doi.org/10.22506/ti%2F2017%2Fv24%2Fi2%2F162426