Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

The Effect of Nitrate on some Biochemical Parameters of Rabbits and Ameliorate its Effect by Using Vitamin E and Rosemary (Rosmarinus Officinalis)


Affiliations
1 Department of Veterinary Public Health, University of Al-Fallujah, Iraq
     

   Subscribe/Renew Journal


This study was conducted to investigate the effect of nitrate in the feed of the rabbits on the total protein, albumin, globulin, cholesterol, triglyceride and urea in serum and to study ameliorate nitrate toxicity by using vitamin E and rosemary. Twenty-eight adult New Zealand rabbits were divided randomly into four groups, Group 1(G2): fed basal ration as a control group. Group 2 (G2): fed ration supplemented with nitrate (168 mg/kg B.W./day), Group 3 (G3): fed ration supplemented with nitrate (168 mg/kg B.W./day) plus vitamin E (100 mg/kg B.W.).Group 4 (G4): fed ration supplemented with nitrate (168 mg/kg B.W./day) plus rosemary (500 mg/kg B.W.). The results showed a significant(P≤0.05) decrease in total protein and globulin in the group that fed nitrate (G2) compared with the control (G1)and groups fed a mixture of vitamin E and rosemary with nitrate(G3 and G4). The cholesterol, triglyceride and blood urea concentration showed a significant increase (P≤0.05)in the group that fed nitrate (G2) compared with the control group (G1), whereas the groups that fed vitamin E and rosemary with nitrate(G3 and G4) showed a significant (P≤0.05) decreased in the parameters measured compared with then itrate fed rabbits (G2). The results illustrated above revealed a remarkable impact of nitrate supplementation causing a significant decrement in protein concentrations along with considerable rise in the cholesterol, triglyceride and blood urea levels which was partially neutralized by vitamin E and rosemary.

Keywords

Nitrate, Rabbit, Rosemary, Vitamin E.
User
Subscription Login to verify subscription
Notifications
Font Size

  • Lijinsky W. N-Nitroso compounds in the diet. Mutat. Res. 1999; 443:129–38.
  • Knobeloch L,Salna B, Hogan A, AndersonH. Blue babies and nitrate-contaminated well water. Environ. Health Perspect. 2000; 108: 675–78.
  • Van Maanen JS, van Dijk A, Mulder K, de Baets MH,Menheere PA, Heide D,et al. Consumption of drinking water with high nitrate levels causes hypertrophy of the thyroid. Toxico. Lett. 1994; 72:365–74.
  • Parada B, Alves R, Piloto N, Sereno J,Figueiredo A, Pinto R, et al.,Teixeira F. Characterization of a rat model of moderate chronic renal failure focus on hematological, biochemical, and cardio-renal profiles. Ren. Fail. 2009; 31: 833-42.
  • Krishnamoorthy P, Sangeetha M. Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in Albino rats. Indian J. Bioch. Biophys.2008; 45: 206-08.
  • Al-Attar A M. Antioxidant effect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice. Saudi J. of Biol. Sci. 2011; 18: 63–72.
  • Warren S, Patel S, KapronCM. The effect of vitamin E exposure on cadmium toxicity in mouse embryo cells in vitro. Toxico. 2000; 142:119–26.
  • Hassan NS,AwadSM. Reverse effect of vitamin e on oxidative stress, derivatives and conductivity changes of hemoglobin induced by exposure to cadmium. J. Appl. Sci. Res.2007; 3: 437-43.
  • Rašković A, Milanović I, Pavlović N, Cebovic T, Vukmirović S, Mikov M. Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotectivepotential. BMC Complementary and Alternative Medicine. 2014; 14:225.
  • Lo A, Liang Y, Lin-Shiau S, Ho C,Lin J. Carnosol antioxidant in rosemary, suppresses inducible nitric oxide synthase through down-regulating nuclear factor-KB in mouse macrophages. J. Carcinogenesis. 2002; 23:983-91.
  • Al-rawi S Th. Environmental impact of nitrate on some physiological and productive traits in dairy cattle. Dissertation, University of Baghdad, Iraq,2014.
  • Alqayim M Aj,AsisSA. Protective role of vitamin E and/or methionine against lead-Induce changes on hematological parameters in rabbits. Iraqi J. Med. Sci.2013; 11: 187-94.
  • Andon AN, Aga MR, Martinez MA, Ares I, Garcia-Risco MR, Senorans FJ,et al. Acute oral safety study of rosemary extracts in rats. J. of Food Prot.2008; 71: 790–95.
  • Tietz NW. Clinical guide to laboratory test. 2nd ed. ; 1999. p. 1096-99.
  • Snedecor GW, Cochran. WG. Statistical Methods. 7th ed. The State University Press American, Iowa, 1989.
  • Atyabi N, Yasini SP, Jalali SM, Shaygan H. Antioxidant effect of different vitamins on methemoglobin production: An in vitro study. Vet. Res. Forum. 2012; 3: 97 – 101.
  • Hansen PR, Taxvig C, Christiansen C, AxelstadM, Boberg J, Kiersgaard MK, et al. Evaluation of endocrine disruptingeffects of nitrate after in utero exposure in rats andof nitrate and nitrite in the H295R and T-screenassay. Oxford J. Life Sci. and Med.Toxicol. Sci. 2009; 108 : 437-44.
  • Bassuny SM, Shehata SA,Bahgat LB,Mohmed SA. Nitrate toxicity in rabbits: Effect of nitrate in drinking water on digestion, some blood constituents and growth performance of growing rabbits. Egyptian J. of Rabbit Sci. 2004; 14: 14758.
  • EL-Tahan NR, Morsi RM, EL-HadadAA. Effect of selenium to high doses of nitrate and nitrite in immunoglubin production and detoxifying enzymes activities. J. of Applied Sci. Res. 2010; 6: 1988-95.
  • Shokrollahi B, Amini F, Fakour S, Andi MA. Effect of rosemary (Rosmarinus officinalis) extract on weight, hematology and cell-mediated immune response of newborn goat kids., J. Agr. Rural Develop. Trop. Subtrop. 2015; 116: 91–97.
  • Silva ICM da, Ribeiro AML, Canal CW, Vieira MM, Pinheiro CC, GonçalvesT, et al. Effect of Vitamin E Levels on the Cell-Mediated Immunity of Broilers Vaccinated Against Coccidiosis., Brazilian J. of Poultry Sci. 2011; 13: 53-6.
  • Al-Sheyab FM, Abuharfeil N, Salloum L, Hani RB,AwadDS. The effect of Rosemary (Rosmarinus officinalis L.) plant extracts on the immune response and lipid profile in mice. J. of Biol. and Life Sci.2012; 3: 37–58.
  • Konjufca VK, Bottje WG, Bersi K, Erf GF. Influence of dietary vitamin E on phagocytic functions of macrophages in broilers. J. ofPoul. Sci.2004; 83:1530-34.
  • Sachar A, RainaS. Effect of inorganic pollutant (Nitrate) on biochemical parameters of the fish, Aspidoparia Morar. I. J. of Inno. Res. in Sci., Eng. and Tech.2014; 3: 12568- 73.
  • Azeez OH, Mahmood MB, Hassan JS. Effect of nitrate poisoning on some biochemical parameters in rats. Iraqi J. of Vet. Sci.2011; 25: 47-50.
  • Labban L, Mustafa U. El-S. IbrahimYM. The effects of Rosemary (Rosmarinus officinalis) leaves powder on glucose level, lipid profile and lipid perodoxation. I. J. of Cl. Med. 2014; 5: 297-304.
  • Evstigneeva RP, Volkov IM, ChudinovaVV. Vitamin E as a universal antioxidant and stabilizer of biological membranes. Membr. Cell Biol. 1998; 12: 151–72.
  • ShourAA, Abd El-Aziz I, Al-Agha M,KerritA. Effect of oral administration of nitrate on serum glucose, some lipids and non-protein nitrogen constituents.,Islamic Un. J. 1999; 7:113.
  • Borras-Linares I, Stojanović Z, Quirantes-Piné R, ArraezRoman D, Svarc-Gajic J, Fernández-Gutiérrez A,et al. Rosmarinus officinalis leaves as a natural source of bioactive compounds. Int. J. Mol. Sci. 2014; 15: 20585-606.
  • Yesil-Celiktas O,Sevimli C, Bedir E, Vardar-Sukan F. Inhibitory effects of rosemary extracts, carnosic acid and rosmarinic acid on the growth of various human cancer cell lines, Plant Foods Hum. Nutr.2010; 65: 158-63.

Abstract Views: 349

PDF Views: 2




  • The Effect of Nitrate on some Biochemical Parameters of Rabbits and Ameliorate its Effect by Using Vitamin E and Rosemary (Rosmarinus Officinalis)

Abstract Views: 349  |  PDF Views: 2

Authors

Saad Thabit Jassim Alrawi
Department of Veterinary Public Health, University of Al-Fallujah, Iraq

Abstract


This study was conducted to investigate the effect of nitrate in the feed of the rabbits on the total protein, albumin, globulin, cholesterol, triglyceride and urea in serum and to study ameliorate nitrate toxicity by using vitamin E and rosemary. Twenty-eight adult New Zealand rabbits were divided randomly into four groups, Group 1(G2): fed basal ration as a control group. Group 2 (G2): fed ration supplemented with nitrate (168 mg/kg B.W./day), Group 3 (G3): fed ration supplemented with nitrate (168 mg/kg B.W./day) plus vitamin E (100 mg/kg B.W.).Group 4 (G4): fed ration supplemented with nitrate (168 mg/kg B.W./day) plus rosemary (500 mg/kg B.W.). The results showed a significant(P≤0.05) decrease in total protein and globulin in the group that fed nitrate (G2) compared with the control (G1)and groups fed a mixture of vitamin E and rosemary with nitrate(G3 and G4). The cholesterol, triglyceride and blood urea concentration showed a significant increase (P≤0.05)in the group that fed nitrate (G2) compared with the control group (G1), whereas the groups that fed vitamin E and rosemary with nitrate(G3 and G4) showed a significant (P≤0.05) decreased in the parameters measured compared with then itrate fed rabbits (G2). The results illustrated above revealed a remarkable impact of nitrate supplementation causing a significant decrement in protein concentrations along with considerable rise in the cholesterol, triglyceride and blood urea levels which was partially neutralized by vitamin E and rosemary.

Keywords


Nitrate, Rabbit, Rosemary, Vitamin E.

References





DOI: https://doi.org/10.22506/ti%2F2017%2Fv24%2Fi1%2F149038