Toxicology International (Formerly Indian Journal of Toxicology)

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Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster


Affiliations
1 Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
2 Laboratorio de Genetica y Biologia Molecular, Departamento de Biologia, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela, Bolivarian Republic of
3 Instituto de Investigaciones Clínicas “Dr. Américo Negrette”, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela, Bolivarian Republic of
     

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We determined the effect of long-term administration (12 days) of minocycline (Mino) on the oxidative stress induced by paraquat (PQ) in D. melanogaster. After intoxication with PQ (40 mM) for 36 hours, the intoxicated group was subdivided in three groups: PQ (no further treatment after PQ intoxication), PQ-Mino (treated with Mino [0.05 mM] for 12 days after intoxication) and PQ-Control (exposed to PQ and maintained in standard medium for 12 days after intoxication). Two additional groups, not treated with PQ, were added: Control and Mino. After 36 hours intoxication the concentrations of H2O2, the activities of superoxide dismutase (SOD), Catalase (CAT), glutathione peroxidase (GSH-Px) and nitric oxide synthase (NOS) in the PQ group were increased, but the levels of nitrite and glutathione (GSH) were reduced with respect to the Control. In the group PQ also were observed an increase in the concentrations of MDA, protein carbonyl and the 8-hydroxydeoxyguanosine (8-OHdG) compared with Control. The post-treatment by 12 days with minocycline (PQ-Mino) mitigated oxidative damage induced by PQ as evidenced by the reduction in the concentration of the oxidative stress markers and by decrease damage to the lipid membrane (MDA), protein (protein carbonyl), DNA (8-OHdG) and in an increase of the extension of the life cycle of the flies with respect to PQ-Control.

Keywords

Drosophila melanogaster, Minocycline, Oxidative Stress, Paraquat.
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  • Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster

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Authors

E. Bonilla
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
S. Medina-Leendertz
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
M. Mora
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
J. R. Vielma
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
L. Atencio-Bracho
Laboratorio de Genetica y Biologia Molecular, Departamento de Biologia, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela, Bolivarian Republic of
Y. Bravo
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
M. Paz
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
A. Leal-Yepez
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
J. L. Arcaya
Instituto de Investigaciones Clínicas “Dr. Américo Negrette”, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela, Bolivarian Republic of

Abstract


We determined the effect of long-term administration (12 days) of minocycline (Mino) on the oxidative stress induced by paraquat (PQ) in D. melanogaster. After intoxication with PQ (40 mM) for 36 hours, the intoxicated group was subdivided in three groups: PQ (no further treatment after PQ intoxication), PQ-Mino (treated with Mino [0.05 mM] for 12 days after intoxication) and PQ-Control (exposed to PQ and maintained in standard medium for 12 days after intoxication). Two additional groups, not treated with PQ, were added: Control and Mino. After 36 hours intoxication the concentrations of H2O2, the activities of superoxide dismutase (SOD), Catalase (CAT), glutathione peroxidase (GSH-Px) and nitric oxide synthase (NOS) in the PQ group were increased, but the levels of nitrite and glutathione (GSH) were reduced with respect to the Control. In the group PQ also were observed an increase in the concentrations of MDA, protein carbonyl and the 8-hydroxydeoxyguanosine (8-OHdG) compared with Control. The post-treatment by 12 days with minocycline (PQ-Mino) mitigated oxidative damage induced by PQ as evidenced by the reduction in the concentration of the oxidative stress markers and by decrease damage to the lipid membrane (MDA), protein (protein carbonyl), DNA (8-OHdG) and in an increase of the extension of the life cycle of the flies with respect to PQ-Control.

Keywords


Drosophila melanogaster, Minocycline, Oxidative Stress, Paraquat.

References





DOI: https://doi.org/10.22506/ti%2F2017%2Fv24%2Fi1%2F149037