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Subacute Oral Toxicity Study of New Formulation of Bacopa monnieri in Rats
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Brahmi (Bacopa Monnieri: BM) is widely used for its memory enhancing properties. The problem with commercially available BM formulations is that, its dose and duration of therapy is high, which indicates; that the permeability of its active components (bacosides) into the brain is meagre and its high doses cause gastric irritation. Hence we hypothesized and prepared a new formulation of BM extract (BME) which can have improved bioavailability so its dose can be low, frequency and duration of therapy is less, to have better tolerability and efficacy. We found that the new lipophylic formulation (20% phosphatidylcholine) of BM had better penetration into the brain due to the increased lipid solubility and had better pharmacokinetic profile than previously marketed formulations. We evaluated subacute toxicity of new formulation of BME and there was no morbidity&mortality, effects on body weight, food consumption, organ weight, urine analysis, hematology, histopathology&serum biochemical parameters. The biochemical parameters like Glucose, Blood Urea, Calcium, SGOT, and SGPT, Creatinine, Cholesterol, Total Bilirubin, Direct Bilirubin and Total Protein doses not shown any significant changes as compared to standard and control group of animals. We can conclude that new formulation of bacopa with 20% phosphotydylcholine is better and safe for oral administration.
Keywords
Bacopa, Phosphatidylcholine, Subacute Toxicity Study.
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- Mukherjee DG, Dey, CD. Clinical trial on Brahmi. I. J Exp Med Sci. 1996; 10:5–11.
- Holcomb LA, Dhanasekaran M, Hitt AR, Young KA, Riggs M, Manyam BV. Bacopa monniera extract reduces amyloid levels in PSAPP mice. J Alzheimers Dis. 2006; 9(3):243–51.
- Husain GM, Mishra D, Singh PN, Rao CV, Kumar V. Ethnopharmacological review of native traditional medicinal plants or brain disorders. Pharmacognocy Review. 2007; 1:20–8.
- Morgan A, Stevens J. Does Bacopa monnieri improve memory performance in older persons? Results of a randomized, placebo-controlled, double-blind trial. J Altern Complement Med. 2010; 16(7):753–9.
- OECD Guidelines for the Testing of Chemicals (No. 407, Section 4: Health Effects) Repeated Dose 28-Day Oral Toxicity in Rodents (Adopted on 12 May 1981 and Updated on 27 July 1995). [cited in 2015]. Available from: http://www.oecd.org/chemicalsafety/testing/37477972.pdf
- Sridevi K, Ravishankar K, Kiranmayi GVN. Evaluation of diuretic and antiurolithiatic activities of ethanolic leaf extract of basellaalba. Int J Pharm. 2014; 4(1):145–9.
- Dash DK, Yeligar VC, Nayak SS, Ghosh T, Rajalingam D, Sengupta P et al. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R.Br. on paracetamol-induced hepatotoxicity in rats. Trop J Pharm Res. 2007; 6(3):755–65.
- Chapter 1. [cited in 2015]. Available from: http://www.rajswasthya.nic.in/RHSDP%20Training%20Modules/ Lab.%20Tech/Histo/Introduction.pdf
- Chitra B, Ramaswamy RS, Suba V. Toxicity Evaluation of Purna Cantirotaya Centuram, a Siddha Medicine in Wistar Rats. Int sch res notices. 2015; 1–10.
- Complete Urinalysis. [cited in 2015]. Available from: http:// www.highlands.edu/academics/divisions/scipe/biology/ labs/cartersville/2122/urinalysis.htm
- Cho SH, Yoon Y, Yang Y. The Evaluation of the Body Weight Lowering Effects of Herbal Extract THI on Exercising Healthy Overweight Humans: A Randomized DoubleBlind, Placebo-Controlled Trial. Evid Based Complementary Altern Med. 2013; 1–8.
- Pattanik N, Singh AV, Panday RS, Singh BS, Kumar M, Dixit SK et al. Toxicological and free radical scavenging property of tamrabhasma. Indian J ClinBiochem. 2003; 18(2):181–9.
- Allan JJ, Damodaran A, Deshmukh NS, Goudar KS, Amit A. Safety evaluation of a standardized phytochemical composition extracted from Bacopa monnieri in Sprague-Dawley rats. Food Chem Toxicol. 2007; 45(10):1928–37.
- Teo S, Stirling D, Thomas S, Hoberman A, Kiorpes A, Khetani V. A 90 day oral gavage toxicity study of d-methylphenidate and d, l-methylphenidate in Sprague–Dawley rats. Toxicology. 2002; 179:183–96.
- Feldman DL, Mogelesky TC, Sharif R, Sawyer WK, Jeune M, Hu CW et al. The in vitro and ex vivo antioxidant properties and hypolipidemic activity of CGP 2881. Atherosclerosis. 1999; 144(2):343–55.
- Cholesterol Abnormalities and Diabetes. [cited in 2015]. Available from: http://www.heart.org/HEARTORG/Conditions/ Diabetes/WhyDiabetesMatters/Cholesterol-AbnormalitiesDiabetes_UCM_313868_Article.jsp
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