Open Access
Subscription Access
Open Access
Subscription Access
Protective Effect of Curcumin Against Imidacloprid-Induced Genotoxicity in Rats
Subscribe/Renew Journal
Aim: To study the effect of curcumin in imidacloprid (IMI)-induced genotoxicity by comet and micronucleus assays.
Materials and Methods: Experiments were conducted on adult rats that were randomly divided into six groups of six rats as follows: Group 1: Sham; Group 2: Curcumin (50 mg/kg po); Group 3: IMI (20 mg/kg po); Group 4: IMI (40 mg/kg po); Group 5: IMI (20 mg/kg po) + curcumin (50 mg/kg po); and Group 6: IMI (40 mg/kg po) + curcumin (50 mg/kg po). Micronucleus and comet assays were used to study the DNA damage in all the groups.
Results: There was a significant (P < 0.05) increase in the comet tail length (μm) and the frequency of micronuclei formation in the Group 4 treated with 40 mg/kg IMI when compared to other groups. Curcumin treatment in Group 6 showed a significant (P < 0.05) decrease in the comet tail length, and micronuclei as compared to Group 4 though the decrease was significantly (P < 0.05) low as compared to other groups.
Conclusions: This study revealed that IMI has a tendency to induce the genotoxicity on exposure to 40 mg/kg b.wt. Curcumin, due to its anti-oxidant and anti-mutagenic property, alleviated the genotoxicity induced by IMI.
Keywords
- Tomizawa M, Casida JE. Neonicotinoid insecticide toxicology: Mechanisms of selective action. Annu Rev Pharmacol Toxicol. 2005; 45:247–68.
- Tomizawa M, Casida JE. Selective toxicity of neonicotinoids Devi, et al.: Curcumin against imidacloprid-induced genotoxicity attributable to specificity of insect and mammalian nicotinic receptors. Annu Rev Entomol 2003;48:339–64.
- Matsuda K, Buckingham SD, Kleier D, Rauh JJ, Grauso M, Sattelle DB. Neonicotinoids: Insecticides acting on insect nicotinic acetylcholine receptors. Trends Pharmacol Sci. 2001; 22:573– 80.
- Chauzat MP, Carpentier P, Martel AC, Bougeard S, Cougoule N, Porta P, et al. Influence of pesticide residues on honey bee (Hymenoptera: Apidae) colony health in France. Environ Entomol. 2009; 38:514–23.
- Abou-Donia MB, Goldstein LB, Bullman S, Tu T, Khan WA, Dechkovskaia AM, et al. Imidacloprid induces neurobehavioral deficits and increases expression of glial fibrillary acidic protein in the motor cortex and hippocampus in offspring rats following in utero exposure. J Toxicol Environ Health A .2008; 71:119–30.
- Karabay NU, Oguz MG. Cytogenetic and genotoxic effects of the insecticides, imidacloprid and methamidophos. Genet Mol Res. 2005; 4:653–62.
- Bal R, Turk G, Tuzcu M, Yilmaz O, Kuloglu T, Gundogdu R, et al. Assessment of imidacloprid toxicity on reproductive organ system of adult male rats. J Environ Sci Health B. 2012; 47:434–44.
- Kulkarni SK, Dhir A. An overview of curcumin in neurological disorders. Indian J Pharm Sci. 2010; 72:149–54.
- Kulkarni S, Dhir A, Akula KK. Potentials of curcumin as an antidepressant. Scientific World Journal. 2009; 9:1233– 41.
- Asare GA, Bugyei K, Sittie A, Yahaya ES, Gyan B, Adjei S, et al. Genotoxicity, cytotoxicity and toxicological evaluation of whole plant extracts of the medicinal plant Phyllanthus niruri (Phyllanthaceae). Genet Mol Res. 2012; 11:100–11.
- Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175:184–91.
- Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, et al. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35:206–21.
- Feng S, Kong Z, Wan X, Peng P, Zeng EY. Assessing the genotoxicity of imidacloprid and RH-5849 in human peripheral blood lymphocytes in vitro with comet assay and cytogenetic tests. Ecotoxicol Environ Saf. 2005; 61:239–46.
- Carmello JC, Pavarina AC, Oliveira R, Johansson B. Genotoxic effect of photodynamic therapy mediated by curcumin on Candida albicans. FEMS Yeast Res. 2015; 15:fov018.
- Biswas J, Roy S, Mukherjee S, Sinha D, Roy M. Indian spice curcumin may be an effective strategy to combat the genotoxicity of arsenic in Swiss albino mice. Asian Pac J Cancer Prev. 2010; 11:239–47.
- Majer BJ, Laky B, Knasmuller S, Kassie F. Use of the micronucleus assay with exfoliated epithelial cells as a biomarker for monitoring individuals at elevated risk of genetic damage and in chemoprevention trials. Mutat Res. 2001; 489:147–72.
Abstract Views: 482
PDF Views: 1