Toxicology International (Formerly Indian Journal of Toxicology)

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2, 5-Hexanedione-Induced Oxidative Damage and DNA Fragmentation:Ameliorative Role of Rutin Ex Vivo


Affiliations
1 Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
2 Department of Food Technology, Federal Institute of Industrial Research, Oshodi, Lagos State, Nigeria
3 Department of Pharmacognosy and Ethnopharmacy, Usmanu Danfodiyo University, Sokoto, Nigeria
     

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The aim of this research was to evaluate the ameliorative effects of rutin on 2, 5-hexanedione-induced oxidative damage and DNA fragmentation in the viscera of Wistar rat ex vivo. Blood and tissues homogenates from normal Wistar rats weighing 150-200 g were used for the present investigation. The treatment with 2,5-hexanedione and/or rutin was for four (4), eight (8) and sixteen (16) hours incubation period at room temperature, and used non-specifically at the final concentrations of 0.0, 10.0 μM, 100.0 μM, 1.0 mM and 10.0 mM respectively. The experiments were done in four sequential stages involving the determination of lipid peroxidation and DNA fragmentation by standard procedures. 2,5-hexanedione dose/time-dependently caused a significant (P < 0.05) oxidative damage in the blood and tissues via lipid peroxidation and DNA fragmentation relative to control. Rutin administration was able to significantly (P < 0.05) suppress the 2,5-hexanedione-induced oxidative and DNA damage. However, the effective toxicity of 2,5-hexanedione and ameliorative effects of rutin were most versus least pronounced in liver (at 10 μM) and pancreas (at 1 mM) homogenates at 8hrs incubation time, respectively. 2,5-hexanedione in the viscera of Wistar rat induces oxidative and DNA damage characterised by higher level of malondialdehyde and DNA fragmentation. However, rutin administration was able to ameliorate these effects ex vivo.

Keywords

2, 5-Hexanedione, Ameliorative, DNA Fragmentation, Lipid Peroxidation, Rutin.
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  • 2, 5-Hexanedione-Induced Oxidative Damage and DNA Fragmentation:Ameliorative Role of Rutin Ex Vivo

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Authors

Aliyu Muhammad
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Ochuko L. Erukainure
Department of Food Technology, Federal Institute of Industrial Research, Oshodi, Lagos State, Nigeria
Ibrahim Malami
Department of Pharmacognosy and Ethnopharmacy, Usmanu Danfodiyo University, Sokoto, Nigeria
Umoru Murtala
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Fadila I. Mora
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Aminu Suleiman
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Rahfat S. Abubakar
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
James A. Kehinde
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Zaynab M. K. Muhammed
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Ephraim E. Alahirah
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria

Abstract


The aim of this research was to evaluate the ameliorative effects of rutin on 2, 5-hexanedione-induced oxidative damage and DNA fragmentation in the viscera of Wistar rat ex vivo. Blood and tissues homogenates from normal Wistar rats weighing 150-200 g were used for the present investigation. The treatment with 2,5-hexanedione and/or rutin was for four (4), eight (8) and sixteen (16) hours incubation period at room temperature, and used non-specifically at the final concentrations of 0.0, 10.0 μM, 100.0 μM, 1.0 mM and 10.0 mM respectively. The experiments were done in four sequential stages involving the determination of lipid peroxidation and DNA fragmentation by standard procedures. 2,5-hexanedione dose/time-dependently caused a significant (P < 0.05) oxidative damage in the blood and tissues via lipid peroxidation and DNA fragmentation relative to control. Rutin administration was able to significantly (P < 0.05) suppress the 2,5-hexanedione-induced oxidative and DNA damage. However, the effective toxicity of 2,5-hexanedione and ameliorative effects of rutin were most versus least pronounced in liver (at 10 μM) and pancreas (at 1 mM) homogenates at 8hrs incubation time, respectively. 2,5-hexanedione in the viscera of Wistar rat induces oxidative and DNA damage characterised by higher level of malondialdehyde and DNA fragmentation. However, rutin administration was able to ameliorate these effects ex vivo.

Keywords


2, 5-Hexanedione, Ameliorative, DNA Fragmentation, Lipid Peroxidation, Rutin.

References





DOI: https://doi.org/10.22506/ti%2F2015%2Fv22%2Fi3%2F137638