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Vidya, A. K.
- A Characterization and Evaluation of Antibacterial Efficacy of Bacteriocin Produced by Bacillus subtilis Isolated from Raw Milk
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Authors
Affiliations
1 Department of Biochemistry, Kongu Arts and Science College, Erode - 638107, Tamil Nadu, IN
1 Department of Biochemistry, Kongu Arts and Science College, Erode - 638107, Tamil Nadu, IN
Source
ScieXplore: International Journal of Research in Science, Vol 2, No 2 (2015), Pagination: 18-23Abstract
This study presents Bacteriocin (antimicrobial compound) produced from Bacillus subtilis isolated from unpasteurized milk. The antibiotic susceptibility tests for B. subtilis isolated from raw milk against various antibiotics demonstrated high sensitivity to Tetracycline, Moderate and less sensitivity towards Streptomycin and Chloramphenicol respectively while being resistant to Penicillin. Culture filtrate containing Bacteriocin showed thermostability (up to 100 °C), expressed a pH tolerance (2.0-10.0) and emerged resistance towards trypsin and pepsin. Partial purification of bacteriocin was performed using ammonium sulphate precipitation and then purified by passing into a column of Silica gel. In SDS-PAGE analysis, purified bacteriocin isolated from Bacillus subtilis showed the molecular weight to be approximately 3.4 KDa. The isolate Bacteriocin was tested finally for antibacterial activity against E. coli, associated with food borne illnesses. The zone of inhibition clearly proved that Bacteriocin has inhibitory effect on the food pathogen E. coli, thus confirming its bactericidal action in food.Keywords
Antibacterial Agent, Bacteriocin, Bacteriocidal, Biopreservative, Bacillus subtilis, E. coli, Micrococcus luteus.Full Text
References
- Cleveland J., Montville T. J., Nes I. F., Chikindas M. L. “Bacteriocins: Safe, natural antimicrobials for food preservation”. Int J Food Microbiol. Vol. 71, p. 1–20, 2001.
- Dhanapathi T. G., Prabhakar P., Prabhakar., “Antibacterial activity of Bacillus subtilis extraction pathogenic organism”. Tamil Nadu J Veterinary and Animal Sciences. Vol. 4(4), p. 150–153, 2008.
- Geis A., Singh J., Teuber M., “Potential of lactic Streptococcus to produce bacteriocin”. Appl Enviro Microbiol. Vol. 51, p. 105–109, 1983.
- Karthikeyan V., Santosh S. W., “Isolation and partial characterization of bacteriocin produced from Lactobacillus plantarum”. Afr J Microbiol Res. Vol. 3, p. 233–239, 2009.
- Klaenhammer T. R., Bacteriocins of lactic acid bacteria. Biochimie. Vol. 70, pp. 337–349, 1998,
- Rane S., Street vended Food in development word: Hazard Analysis. Indian J Microbiol, Vol. 51(1), p. 100–106, 2011.
- Sharp R. J., Scawen M. D., Atkinson T. Fermentation and downstream processing of Bacillus. Biotechnology Handbook: Bacillus (Harwood CR, ed), Plenum Press, New York, p. 255–292, 1989.
- Stein T., Bacillus subtilis antibiotics: Structures, syntheses and specific functions. Mol Microbiol. Vol. 56, p. 845–857, 2005.