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Affas, Shaza
- Direct Spectrophotometric Determination of Sildenafil Citrate in Pharmaceutical Preparations via Complex formation with Two Sulphonphthalein Acid Dyes
Authors
1 Analytical and Food Chemistry Department, University of Aleppo, SY
Source
Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1191-1196Abstract
Objective: Simple, sensitive and free- extraction spectrophotometric methods have been developed for the determination of Sildenafil citrate (SIL) both in pure and in pharmaceutical forms. Methods: Two spectrophotometric methods are based on the formation of yellow colored ion-pair complexes between the studied drug and two sulphonphthalein acid dyes, bromocresol green (BCG)(method A) and bromothymol blue (BTB) (method B) with absorption maxima at 414 and 409 nm, respectively.
Results: The effects of analytical parameters on the reported systems were investigated. The complexation reactions were extremely rapid at room temperature and the absorption values remain unchanged up to 24h. Various analytical parameters have been evaluated and the results have been validated by statistical. Beer's law was obeyed in the concentration ranges of (1.33 - 50) μg/ml for method A and (0.8 -27) μg/ml for method B, the limits of detection were 0.093 and 0.059 μg/ml and the molar absorptivity coefficients were 2.28×104 and 3.05 ×104 L .moL-1.cm-1 for method A and B respectively.
Conclusion: The proposed methods were successfully applied to the analysis of commercial tablets containing the drugs. Two methods were free-extractive, rapid, and have high sensitivity comparing with other previous direct spectrophotometric methods. Interferences of the other ingredients and excipients were not observed.
Keywords
Spectrophotometry, Sildenafil Citrate, Bromocresol Green, Bromothymol Blue, Ion-Pair Complex.
- New Simple Spectrophotometric Method for the Simultaneous Estimation of the Mixtures of Sildenafil and some Serotonin Reuptake Inhibitors
Authors
1 Analytical and Food Chemistry Department, University of Aleppo, SY
Source
Research Journal of Pharmacy and Technology, Vol 12, No 2 (2019), Pagination: 711-716Abstract
This work presents a simple, reproducible, sensitive, generic and efficient method for simultaneous estimation. Vierordt’s method or simultaneous equation method was developed and validated for simultaneous estimation of two mixtures: Sildenafil and Duloxetine, Sildenafil and Fluoxetine, in the pure and pharmaceutical dosage form. The method was based on the measurement of absorbance at two wavelengths for each mixture in methanol. Calibration curves of all drugs were found to be linear. With good correlation coefficient values more than 0.99. The RSD values were found within limits and the recoveries ranging between 98 to 102% that confirms that the proposed method is accurate. This method for simultaneous estimation of these four mixtures is simple and economical and can be applied successfully for the simultaneous estimation of PDEs inhibitors and their combinations with some serotonin reuptake inhibitors in the pure and pharmaceuticals dosage forms.Keywords
Simultaneous Estimation, Spectrophotometric, Vierordt’s Method, Sildenafil, Serotonin Reuptake Inhibitors.References
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