Research Journal of Pharmacy and Technology

M. Sasikala ¹, R. Sundaraganapathy ², S. Mohan ¹

Affiliations
1 Karpagam College of Pharmacy, Coimbatore – 641032, Tamil Nadu, IN
2 Karpagam Academy of Higher Education, Coimbatore – 641021, Tamil Nadu, IN

Abstract

Introduction: Since to meet the worlds challenging diseases, there is a huge requirement for finding remedies. The remedy might be derived from any source like synthetic, mineral, biological, microbiological, marine and herbal. Among which, herbal, the natural remedy stands first to treat an illness without side effects. The active secondary metabolites have been spread widely across the world. The herb that acts both as nutraceutical and as pharmaceutical majorly is various species of spinach. Hence, the water spinach is selected for getting an excellent moiety by means of different analytical procedures. Aim and Objectives: The aim is to characterize isolated herbal compound from Ipomoea aquatica Forsskal with the use of spectroscopic and chromatographic techniques. Method: The authenticated herb was extracted for its active metabolites. The screened phytochemicals by preliminary identification tests and TLC were applied for isolation. The high yield isolate from water fraction was evaluated for characterizing its chemical and biological properties. The structural elucidation was done with FTIR, ¹H NMR and LCMS. The biological property was analyzed by colorimetric method using MCF-7 cell lines. Results and Discussion: The yield of isolate was 13.9 %w/w. The compound was characterized as halo aromatic alcoholamide with molecular weight of 1677.60 g/mol. IC50 value of active molecule by MTT assay was detected as 173.1 μg/ml. Conclusion: The invented anticancer natural drug might be isolated in bulk to prepare herbal formulations. The medicine could be utilized for treating various life threatening diseases because of its functional groups and pharmacological actions.

Keywords

Isolation, MCF-7, Water Spinach, FTIR, LCMS, NMR.

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