Research Journal of Pharmacy and Technology

S. Geetha ¹, S. Rajeswari ¹

Affiliations
1 Department of Biochemistry, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Chennai, Tamil Nadu, IN

Abstract

A preliminary investigation was done to screen the phytochemical contents and to find out the proximate composition and antibacterial activity of Andrographis paniculata seeds. Standard analytical procedures were followed and four different solvent extractions were done. Qualitative phytochemical screening of Andrographis paniculata seeds showed the presence of important phytochemical compounds in methanol seed extract viz., Flavonoids, Saponins, Tannins, Phenolic compounds, Terpenoids and Glycosides. Chloroform, ethyl acetate and methanol extracts reported the presence of tannins, Phenols and terpenoids. Flavonoids were present in chloroform and methanol extracts. The hexane extract did not show positive for any of the tests. Alkaloids was found to be absent in all the solvent extracts. Further, tannin concentration was found to be in high in methanol extract in comparison to other solvent extracts. Proximate composition recorded methanol extractive value of about 57.75% and was higher than water extractive value (46.23%). The moisture content of the crude drug was found to be 21.40% and foaming index was < 100%. Antibacterial activity was studied by assessing the minimum inhibitory concentration of methanol extract for bacterial strains. The MIC values of Salmonella typhi 59, Vibrio cholera and Shigellaboydii 8 was found to be 100 μg/ml and for Bacillus licheniformis and Staphylococcus aureus, MIC was found to be 10μg/ml. The preliminary investigation suggests that Andrographis paniculata seed extract have a rich profile of phytochemicals with significant proximate composition and good antibacterial activity. The findings can be taken as an initiative step for finding out the promising agents responsible for its phytochemical and antibacterial activities. This could further pave way to the research with respect to antioxidant studies of Andrographispaniculata seeds.

Keywords

Andrographis paniculata Seed Extract, Phytochemicals, Antibacterial, Antioxidant, MIC (Minimum Inhibitory Concentration).

Full Text

     

T. Karpagam ¹, Jannathul Firdous ², Revathy ¹, Shanmuga Priya ¹, B. Varalakshmi ¹, S. Gomathi ¹, S. Geetha ³, Noorzaid Muhamad ²

Affiliations
1 Department of Biochemistry, Shrimati Indira Gandhi College, Tiruchirappalli, IN
2 Cluster for Integrative Physiology and Molecular Medicine (CIPMM), Universiti Kuala Lumpur Royal College of Medicine Perak, No.3, Jalan Greentown, 30450, Ipoh, Perak, MY
3 Biochemistry Department, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, IN

Abstract

In the present study, anticancer and cytotoxicity of Aloe vera ethanolic leaves extract was evaluated as the leaves of A.vera are reported to have great medicinal value with potential therapeutic applications. The anticancer activities of the ethanolic leaves extract of A.vera were investigated using 3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide assay on three human cancer cell lines HepG2 (liver cancer), HeLa (human cervical carcinoma cell line) and A549 (human lung adenocarcinoma epithelial cell line).In anticancer studies, A.vera ethanolic leaves extract showed potent proliferation inhibitory activity against HepG2, HeLa and A549 cell lines. The results have shown that the A.vera ethanolic leaves extract contain some active ingredients with the potential of being anticancer agents. A.vera ethanolic leaves extract has the potential to fight against cancer cells. Further work should be carried out on the characterization of specific anticancer components of A.vera.

Keywords

Aloe vera, Cancer cell lines, Lipid peroxidation, MTT assay, Phytochemicals.

Full Text