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Paramita, Rafika Indah
- In Silico Study and In Vitro test of Extract Kaempferia pandurata Roxb. as Anti ER (+) Breast Cancer Cell Line MCF-7
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Authors
Fadilah
1,
Lowilius Wiyono
2,
Brenda Cristie Edina
2,
Risya Amelia Rahmawati
2,
Linda Erlina
1,
Aryo Tedjo
1,
Rafika Indah Paramita
3
Affiliations
1 Departement of Medical Chemistry, University of Indonesia, ID
2 University of Indonesia, ID
3 Indonesian Medical Education and Research Institute, University of Indonesia, ID
1 Departement of Medical Chemistry, University of Indonesia, ID
2 University of Indonesia, ID
3 Indonesian Medical Education and Research Institute, University of Indonesia, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2391-2395Abstract
Breast cancer most commonly occurs in women worldwide, in 2012 there were 1.670.000 cases with the second most prevalence in the world (25% of the total incidence of cancer). Drugs used so far have not been effective and many resistance occurs, it is necessary to a discovery of herbs that contain polyphenols as anticancer. Kaempferia pandurata Roxb plant has been identified to contain various essential oils and several flavonoid compounds that have demonstrated many biological activities. This research was done by simulation study with molecular docking approach which then extraction, the result of the extract was tested in vitro activity against Breast Cancer Cell Line MCF-7. The docking results show that the flavonoid compound against the target ERbreast cancer has an enthalpy energy ΔG -11.75 kcal/mol, the phytochemical results support that the flavonoids compound dominates the composition of the Kaempferia pandurata Roxb. The Result of in vivo test with MTT method IC50 value of standard cisplatin, hexane extract was 8.42; 26.70 μg/ml respectively to MCF-7 cell line showed that Kaempferia pandurata Roxb extract very potential as an antibreast cancer.Keywords
Molecular Docking, Phytochemical Test, Antibreast Cancer, Kaempferia pandurata Roxb., MCF-7 Cell Line.
References
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- Synthesis Nanoparticle extract of Clove (Syzygium aromaticum L.) and Characterization by Differential Scanning Colorimetry Profiling and its Activities as Inhibitor of HeLa Cell lines
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Authors
Fadilah
1,
Vallas Aditiar Widodo
2,
Rahardi Prasetia Priawan
2,
Ericko Ongko Joyo
2,
Hadin Abdurrohman
2,
Fatmawaty
1,
Rafika Indah Paramita
1,
Norma Nur Azizah
3
Affiliations
1 Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, ID
2 Medical Student, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, ID
3 Researcher of Cluster Drug Development Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, ID
1 Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, ID
2 Medical Student, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, ID
3 Researcher of Cluster Drug Development Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3355-3358Abstract
Herbs and spices have been used for thousands of years as medicines. But their have hydrophobic properties, poor stabilities and low bio availabilities, nanoparticles are possible since the interaction of the particle surface with the solvent is strong enough to overcome density differences. DSC melting profiles are essential for identifying the state of the drug, which can significantly influence bioavailability, stability, and water content. The aim of research analysed of nanoparticle extract of Clove (Syzygium aromaticum L.) by Differential Scanning Colorimetry and their activity toward HeLa cell line. The result of synthesis showed that nanoparticle extract with yield 75% yield. In-vitro test results showed that the IC50 of nanoparticle extract and ethanol extract 6.2±2.1 μg/ml, 22.53±1.1μg/ml respectively. The results of this test indicate the synthesis of nanoparticle with traditional herbal medicine may provide a very useful tool in designing future herbal medicine with improved bioavailability profile and less toxicity. That the combination a high potential as a anti cervical cancer compound.Keywords
Nanoparticle, Extract Syzygium aromaticum L, DSC, HeLa Cell Line, MTT Assay.References
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