Research Journal of Pharmacy and Technology

Annapurna, Mukthinuthalapati Mathrusri

• Determination of Raltegravir by Derivative and Difference Spectrophotometric Techniques in Film Coated Tablets

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Authors

Mukthinuthalapati Mathrusri Annapurna ¹, Gunnam Ravi Teja ¹, Ponnekanti Krishna Phani ¹, Sri Boni Chaitanya ¹

Affiliations
1 Department of Pharmaceutical Analysis & Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 991-997

Abstract

Eight simple spectrophotometric techniques have been proposed for the determination of Raltegravir in pharmaceutical preparations. Three zero order (D₀), three first order derivative (D_!) and two difference spectrophotometric methods have been developed in for the determination of Raltegravir in borate buffer (pH 9), hydrochloric acid and sodium acetate buffer solutions. Linearity has been followed over the concentration range 1-150, 10-150 and 10-150 μg/mL in all the buffers in zero order, first order derivative and difference spectroscopy respectively. All the methods were validated and can be very successfully applied for the determination of Raltegravir in pharmaceutical formulations.

Keywords

Raltegravir, Spectrophotometry, Zero Order (D₀), First Order Derivative (D_!), Difference Spectroscopy, Validation.

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• New Validated RP-HPLC Method for the Determination of Vilazodone Hydrochloride–A Serotonergic Anti-Depressant

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Authors

Mukthinuthalapati Mathrusri Annapurna ¹, Kunala Anusha ¹, Afreen Shahena Sharief ¹, Varanasi Sindhuri ¹

Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1077-1080

Abstract

A new simple liquid chromatographic method has been established for the determination of Vilazodone hydrochloride in pharmaceutical formulations. Vilazodone hydrochloride is a strong dopamine antagonist. Vilazodone hydrochloride was approved by the FDA for use in the United States to treat major depressive disorder in January 21, 2011. A mobile phase containing0.1M Ammonium formate: Methanol (20:80, v/v) with flow rate 0.7 mL/min was used for the present chromatographic study(UV detection 241 nm). Linearity was followed over the concentration range 0.1-120 μg/mL. Forced degradation studies were performed by exposing the drug Vilazodone hydrochloride to alkaline, acidic, and oxidation stress degradations. The method was validated as per ICH guidelines (ICH Guidelines 2005).

Keywords

Vilazodone Hydrochloride, RP-HPLC, Stability-Indicating, Validation.

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• Determination of Cabazitaxel (An Anti-Prostate Cancer Agent) by Reverse Phase Liquid Chromatography

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Authors

Mukthinuthalapati Mathrusri Annapurna ¹, Bukkapatnam Venkatesh ¹, Sunkara Mrunal Chaitanya ¹

Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1138-1144

Abstract

Cabazitaxel is used in the treatment of hormone-refractory prostate cancer. It is a semisynthetic taxane that can be used as a precursor molecule obtained from yew tree needles. The authors have developed a stability indicating liquid chromatographic method for the determination of Cabazitaxel in pharmaceutical products. Shimadzu Model CBM-20A/20 Alite HPLC system (PDA detector) with Zorbax SB C₁₈ column (150 mm × 4.6 mm i.d., 3.5 μm particle size) was selected for the quantification of Cabazitaxel. A mixture of tetra butyl ammonium hydrogen sulphate and methanol was used as the mobile phase with a flow rate of 1.0 mL/min (UV detection at 210 nm). The proposed analytical method was statistically validated and forced degradation studies were conducted. Cabazitaxel has shown linearity over the concentration range 0.1-200 μg/mL with regression equation y = 26145x + 22943 (r²=0.9997) and considerable degradation was observed in acidic, alkaline and oxidation conditions. The method is specific as the resolution of Cabazitaxelwas good in presence of its degradation products. This validated stability indicating liquid chromatographic method can be used for the determination of Cabazitaxel in biological studies as well as for the pharmaceutical products.

Keywords

Cabazitaxel, RP-HPLC, Tetra Butyl Ammonium Hydrogen Sulphate, Validation, Stabilityindicating.

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• First Order Derivative Spectrophotometric Methods for the Determination of Fluorometholone in Ophthalmic Preparations

Abstract Views :169  |  PDF Views:1

Authors

Bulusu Ravi Teja ¹, Bhupathi Raju Kishan Varma ¹, Chandaka Prasanna Kumar ¹, Mukthinuthalapati Mathrusri Annapurna ¹

Affiliations
1 Department of Pharmaceutical Analysis & Quality Assurance, GITAM Institute of Pharmacy,GITAM University, Visakhapatnam-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1145-1148

Abstract

Three simple spectrophotometric techniques have been developed for the quantification of Fluorometholone in ophthalmic preparations using sodium acetate buffer (pH 4), phosphate buffer (pH 4) and phosphate buffer (pH 7) solutions. Linearity was observed over the concentration range 2-25, 2-30 and 1-50 μg/mL in sodium acetate buffer, phosphate buffer (pH 4) and phosphate buffer (pH 7) respectively. The three methods were validated and can be applied for the assay of Fluorometholone in pharmaceutical formulations.

Keywords

First Order Derivative Spectrophotometry, Fluorometholone, Validation.

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• A New Stability Indicating RP-HPLC Method for the Determination of Apremilast-An Antirheumatic Drug

Abstract Views :212  |  PDF Views:1

Authors

Mukthinuthalapati Mathrusri Annapurna ¹, Debi Prasad Pradhan ¹, Malineni Sushmitha ¹

Affiliations
1 Department of Pharmaceutical Analysis & Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1160-1164

Abstract

A novel and simple reverse phase liquid chromatographic method has been established for the determination of Apremilast in pharmaceutical dosage forms. Apremilast is used to treat psoriatic arthritis. The proposed work was performed on Shimadzu Model CBM-20A/20 Alite with Intersil ODS3 C₁₈ column (250 mm × 4.6 mm i.d., 5 μm particle size). A mixture of 0.1% acetic acid and acetonitrile was used as mobile phase in this method with flow rate 0.8 ml/min (UV detection at 203 nm) and the method was validated as per ICH guidelines. The linear regression equation was found to bey = 78597x - 14159 with correlation coefficient 0.9999. Forced degradation studies were performed by exposing the drug Apremilast to acidic, alkaline, oxidation and thermal stress degradations. The proposed RP-HPLC method was found to be robust and specific and this method is suitable for the assay of pharmaceutical dosage forms as well as kinetic studies.

Keywords

Apremilast, RP-HPLC, Validation, Stability-Indicating.

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• Simultaneous Determination of Ketorolac Tromethamine and Fluorometholone in Eye Drops by Spectrophotometry

Abstract Views :138  |  PDF Views:1

Authors

Mukthinuthalapati Mathrusri Annapurna ¹, Vellanki S. V. Sevyatha ¹, Malineni Sushmitha ¹

Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1179-1183

Abstract

Four simple spectrophotometric methods have been developed for the simultaneous determination of Fluorometholone and Ketorolac tromethamine in ophthalmic preparations. Simultaneous equation method, absorbance ratio method (Q - Analysis), first derivative method and multicomponent mode of analytical techniques were selected for the determination of Fluorometholone and Ketorolac tromethamine in phosphate buffer (pH 2.0) solution. Fluorometholone and Ketorolac tromethamine has followed linearity over the concentration range 0.1-20 μg/mL and 1-20 μg/mL respectively. The methods were validated and applied for the simultaneous determination of Fluorometholone and Ketorolac tromethamine in the available pharmaceutical formulations i.e. eye drops.

Keywords

Fluorometholone, Ketorolac Tromethamine, Spectrophotometry, Simultaneous Equation Method, Simultaneous Derivative, Multicomponent Analysis, Q-Analysis, Validation.

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• Forced Degradation Studies of Agomelatine:Development and Validation of Stability Indicating RP-HPLC Method using Internal Standard

Abstract Views :165  |  PDF Views:1

Authors

Mukthinuthalapati Mathrusri Annapurna ¹, Bukkapatnam Venkatesh ¹, Angirekula Narendra ¹

Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 703-707

Abstract

Agomelatine is an anti-depressent drug. A new simple stability indicating reverse phase liquid chromatographic method has been established for the determination of Agomelatine in presence of an internal standard. Agomelatine is used for the treatment of prostate cancer. The proposed work has been performed on Shimadzu Model CBM-20A/20 Alite with Phenomenex C18 column (250 mm × 4.6 mm i.d., 5 μm particle size) using 0.1% formic acid and acetonitrile mixture as the mobile phase with flow rate 0.6 ml/min (UV detection at 205 nm). The method was validated as per ICH guidelines and the regression equation was found to be y = 0.2084x - 0.0475. Agomelatine was subjected to acidic, alkaline, oxidation, UV and thermal stress degradations and the method was reported to be robust and specific and can be applied for the assay of pharmaceutical formulations.

Keywords

Agomelatine, RP-HPLC, Validation, Stability-Indicating, Bimatoprost.

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• New Analytical Techniques for the Determination of Epalrestat in Pharmaceutical Dosage forms by Spectrophotometry

Abstract Views :159  |  PDF Views:1

Authors

Mukthinuthalapati Mathrusri Annapurna ¹, Malineni Sushmitha ¹, Kunala Anusha ¹

Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 739-742

Abstract

Epalrestat is an aldose reductase inhibitor. Three simple, precise and accurate Spectrophotometric methods have been developed for the determination of Epalrestat in pharmaceutical formulations. Spectrophotometric analysis was performed and linearity was observed over the concentration range 0.1-25, 0.5-20 and 0.5-20 μg/ml in phosphate buffer (pH 5.0), phosphate buffer (pH 7.0) and borate buffer (pH 9.0) respectively. All the three methods were validated and can be used for the determination of Epalrestat in pharmaceutical formulations (Tablets).

Keywords

Epalrestat, Phosphate Buffer and Borate Buffer, Spectrophotometry, Validation.

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• Stability Indicating HPLC-DAD Method for the Determination of Oxcarbazepine-An Anticonvulsant

Abstract Views :168  |  PDF Views:0

Authors

Debi Prasad Pradhan ¹, Mukthinuthalapati Mathrusri Annapurna ¹

Affiliations
1 Department of Pharmaceutical Ananlysis & Quality Assurance, GITAM Institute of Pharmacy, GITAM(Deemed to be University), Vishakapatnam, Andhra Pradesh-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 12, No 2 (2019), Pagination: 723-728

Abstract

Oxcarbazepine is an anticonvulsant drug used for the treatment of epilepsy. It acts by decreasing the abnormal electrical activity in the brain. A new stability indicating HPLC method was developed for the quantification of Oxcarbazepine in tablets. Waters e2695 Separations module with Waters 2489 UV detector with Hypersil BDS C18 column (250 mm x 4.6 mm, 5μ) was operated with column temperature 50°C. The detector was monitored at 215 nm and the chromatographic study was run for 20 min. Linearity was observed over 5.0-150 μg/mL with linear regression equation y = 47,910x + 6493.8 with correlation coefficient R² = 0.9999. The LOQ and LOD were found to be 4.5129 μg/mL and 1.4898 μg/mL respectively. Forced degradation studies were performed for Oxcarbazepine capsules and the method was validated as per ICH guidelines.

Keywords

Oxcarbazepine, RP-HPLC, Stability Indicating, Validation, ICH Guidelines.

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• Enantiomeric Separation and Validation of D-Isomer in Pemetrexed Disodium–An Anti-Cancer Agent using Chiral HPLC

Abstract Views :153  |  PDF Views:0

Authors

S. Hemchand ¹, R. Ravi Chandra Babu ¹, Mukthinuthalapati Mathrusri Annapurna ²

Affiliations
1 GITAM Institute of Science, GITAM(Deemed to be University), Vishakapatnam, IN
2 Department of Pharmaceutical Ananlysis & Quality Assurance, GITAM Institute of Pharmacy, GITAM(Deemed to be University), Vishakapatnam, IN

Source

Research Journal of Pharmacy and Technology, Vol 12, No 2 (2019), Pagination: 773-786

Abstract

Pemetrexed disodium is an anti-cancer agent. It is an anti-folate drug used for the treatment of malignant pleural mesothelioma and non-small cell lung cancer. In the present study the authors have proposed a chiral HPLC method for the separation and determination of Pemetrexed disodium and its D-isomer using Chiralpak AD-H (250 x 4.6 mm, 5 μm) column within a run time of 30 mins. A mixture of n-Hexane: Ethanol: Isopropyl alcohol: TFA (250:650:100:1) was the final optimized mobile phase composition after many trials for the separation of D-isomer of Pemetrexed disodium (at 35°C) using Waters Alliance 2695 series HPLC system with 2998 photodiode array detector (UV detection at 240 nm). The method is very much useful for the pharmacokinetic studies as well as the metabolite study in vitro and in vivo for the determination of therapeutic activities of optical isomers.

Keywords

Pemetrexed Disodium, HPLC, D-Isomer, Validation.

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• Janaki Pathi P, Saifulla Khan P, Raveendra Reddy P and Appala Raju N. Visible spectrophotometric estimation of Pemetrexed disodium in pharmaceutical formulations. Journal of Pharmacy Research 4(2); 2011: 524-525.
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• ICH Q2 (R1) Validation of analytical procedures: Text and methodology (2005).

• New Stability Indicating RP-Ultra Fast Liquid Chromatographic Method for the Determination of Perampanel–An Antiepileptic Drug

Abstract Views :153  |  PDF Views:0

Authors

Mukthinuthalapati Mathrusri Annapurna ¹, Rajani Kaibada ¹, Mandula Srinivas ¹

Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2657-2663

Abstract

A new RP-UFLC method has been developed and validated for the determination of Perampanel in its capsules using Shimadzu Model CBM-20A/20 Alite UFLC system (Shimadzu Co., Kyoto, Japan) equipped with SPD M20A prominence photodiode array detector on C8 (2) 100A (Luna) column (250 mm×4.60 mm i.d. 5μm particle size)maintained at room temperature. Mobile phase consisting of a mixture of water, acetonitrile and 0.1% acetic acid (50:50:0.1 v/v) was chosen with a flow rate of 1 mL/min (UV detection at 215nm) on isocratic mode. Perampanel obeys Beer-Lambert’s law over the concentration range 0.05–120 μg/mL with linear regression equation y=73084x+13800 (r²=0.9999). Forced degradation studies were performed and the method was validated as per ICH guidelines.

Keywords

Perampanel, RP-UFLC, Stability Indicating, Validation, ICH Guidelines.

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References

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• New RP-UPLC method for The Simultaneous Determination of Haloperidol and Benzhexol in Tablets

Abstract Views :161  |  PDF Views:0

Authors

Rajesh Pudi ¹, Mukthinuthalapati Mathrusri Annapurna ¹

Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2847-2850

Abstract

Haloperidol is an anti-psychotic drug and Benzhexol (Trihexyphenidyl) is an anticholinergic drug. A newstability indicating UPLC method has been developed for the simultaneous determination of Haloperidol and Benzhexol and the method was validated. Waters UPLC with Empower software, with Acquity UPLC SB C18 x 1.7μ. column and PDA detector was used for the simultaneous determination of Haloperidol and Benzhexol. A mixture of phosphate buffer and Acetonitrile taken in the ratio 56:44 v/v was used as mobile phase with a flow rate of 0.2 ml/min (UV detection at 245 nm) (Injection volume 1μl). Haloperidol and Benzhexol have shown linearregression equations, y=13361x+476.2(R² = 0.9998) and y =10609 x + 899.5 (R² = 0.9998). The LOD and LOQ are found to be 0.03 μg/mL and 0.10 μg/mL for Haloperidol and 0.03μg/mL and 0.09 μg/mL for Benzhexolrespectively. The present liquid chromatographic method is precise, accurate and can be used for the simultaneous estimation of Haloperidol and Benzhexol tablets.

Keywords

Haloperidol, Benzhexol (Trihexyphenidyl), RP-UPLC, Isocratic Mode, Validation.

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References

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• Stability Indicating LC-TQ-ESI-MS Method for the Quantification of Racecadotril in Presence of its Degradants

Abstract Views :371  |  PDF Views:0

Authors

Debi Prasad Pradhan ¹, Mukthinuthalapati Mathrusri Annapurna ¹

Affiliations
1 Department of Pharmaceutical Analysis & Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra Pradesh-530045, IN

Source

Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3437-3443

Abstract

Racecadotril is an anti-diarrheal drug. Waters Alliance e2695 with PDA detector (2998) Zorbax SB C18 column (150 x 4.6 mm, 3.5 μm) (Isocratic mode) was used for the quantification of Racecadotril. A mixture of water and Acetonitrile (50:50) was used as diluent and the injection volume was 20 μl. The flow rate was 1.0mL/min (Detection wave length 230 nm). Linearity was evaluated in the concentration range 1-360 μg/ml with regression equation y = 14710 x+9958.9(Correlation coefficient R² = 0.9996). The LOD and LOQ were found to be 0.2942 μg/mL and 0.9694 μg/mL respectively. Forced degradation studies were conducted for Racecadotril capsules and method validation was performed as per ICH guidelines.

Keywords

Racecadotril, LC-TQ-MS, Forced Degradation Studies, Validation, ICH Guidelines.

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References

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