Research Journal of Pharmacy and Technology

Manisha Mohapatra ¹, Uday C. Basak ¹

Affiliations
1 Seed Bank and Seed Biology Division, Regional Plant Resource Centre, R and D Institute of Forest and Environment Department, Govt. of Odisha,, IN

Abstract

Embelin, one of significant bio-active benzoquinone compound, acts as active principle in fruits of Embelia species and has significant role as a potent source for several formulations. Aim of current research study was to estimate the variation in embelin content different non fruit parts of Embelia tsjeriam-cottam (leaf, stem bark and roots) from both wild and cultivated sources. This was achieved by using spectroscopic method and high performance liquid chromatography (HPLC) analysis method, after purification through column chromatography (CC) and preparative thin layer chromatography technique (PTLC). In case of spectrophotometric analysis, wild Embelia tsjeriam-cottam plants showed embelin content in between 1.14-1.96% dry wt. and the cultivated plants 0.92-1.70% dry wt. Similarly in case of HPLC analysis, wild E. tsjeriam-cottam plants possessed embelin content in a range of 0.033-0.098% dry wt. and cultivated plants yielded embelin in between 0.021-0.071% dry wt. In both cases, amongst all the selected non fruit plant parts, the chloroform extracted root samples possessed highest and the methanol extracted leaf samples yielded lowest embelin content. When comparison was done between the wild and cultivated plant parts in terms of embelin content, wild plants were ascertained to be highly potent than the cultivated plants and root samples possessed highest embelin content followed by stem bark and leaf parts.

Keywords

Cultivated, Embelia tsjeriam-cottam, Embelin, HPLC, Spectrophotometer, Wild.

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