Research Journal of Pharmacy and Technology

Henny Rochaeny ¹, Candra Irawan ¹, Lintannisa Rahmatia ¹, Nilna Izzatul Mawaddah ¹, Ismail Dwi Saputr ²

Affiliations
1 Department of Analytical Chemistry Politeknik AKA Bogor, Bogor 16158, ID
2 PT. Equilab International, Jakarta 12430,, ID

Abstract

We aimed to investigate the performance of the development method for sitagliptin quantification analysis in human plasma by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS) using liquid-liquid extraction (LLE) and Nebivolol as internal standard (IS). The parameters of this presented validation method are selectivity, the lower limit of quantification (LLOQ), linearity, accuracy, precision with five different concentration (LLOQ, Low QC, Medium QC, High QC, Upper Limit Of Quantification (ULOQ)), Integrity of dilution, matrix effect, and test for stability. Based on this study, the multiple reaction monitoring (MRM) transitions were m/z 408,23 → 127,02 for Sitagliptin and m/z 406,25 → 151,06 for IS Nebivolol. The selectivity test obtained % interference of sitagliptin and IS nebivolol by (0.00 - 0.38) % and (0.04 - 0.24) %, respectively. LLOQ test obtained a concentration value of 10.32 ng/mL and % RSD (n = 5) of 5.27 %. When the Sitagliptin concentration ranged from 1 to 1000 ng/mL, the method showed strong linearity with a coefficient of correlation of 0.9991. Accuracy test obtained % differentiation of (-12.58 - 7.77) % and precision test obtained % RSD of (1.62 - 5.32) %. The dilution integrity test obtained % differentiation at 4 and 2 times dilution was -9.20 % and -9.16 %. Matrix effect and stability data were in line with the stipulated European Medicines Agency (EMA) guidelines for validating the bioanalytical method.

Keywords

Sitagliptin, Human Plasma, Nebivolol, Liquid-liquid Extraction, UPLC-MS, Validation.

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Imalia Dwi Putri ¹, Candra Irawan ¹, Maman Sukiman ², Lintannisa Rahmatia ³, Erna Styani ², Aulia Novianti ¹, Avisani Dewanta ³

Affiliations
1 Department of Food Nanotechnology Politeknik AKA Bogor, Bogor 16158,, ID
2 Department of Industrial Waste Treatment Politeknik AKA Bogor, Bogor 16158, ID
3 Department of Analytical Chemistry Politeknik AKA Bogor, Bogor 16158,, ID

Abstract

The utilization of stone bananas is still very minimal, especially banana peels which will only end up as waste. Despite the fact that stone bananas are a fruit high in metabolites, they have numerous health benefits.The banana peels (Musa balbisiana Colla) was extracted with ultrasound-assisted extraction to determine the content of secondary metabolites, total fernol, antioxidant capacity using the DPPH and FRAP methods, as well as the potential for antigout activity in the extract. Ultrasound-assisted extraction is a less expensive, simpler, and more reliable alternative to traditional extraction methods. Musa balbisiana Colla banana peel extract contains alkaloids, saponins, tannins, steroid glycosides and phenols as secondary metabolites. The results obtained from determining the total phenol content was 397.50±0.41 GAE/g sample. Then the results of testing for free radical scavenging activity with the DPPH and FRAP methods showed IC₅₀ values of 143.97±0.17mg/L and 151.08± 0.32mg/L, respectively. Meanwhile the antigout activity was obtained 27.95±0.08% which was greater than Alupurinol, so that Musa balbisiana Colla banana peel has the potential as an alternative compound to reduce uric acid levels.

Keywords

Ultrasonic-Assisted Extraction, Banana Peels, Total Phenol, Antioxidant, Anti-gout.

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