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Jeyanthi Rebecca, L.
- Evaluation of Genetic Diversity among Ocimum sanctum
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1 Department of Industrial Biotechnology, BIHER, Selaiyur, Chennai-600073, IN
1 Department of Industrial Biotechnology, BIHER, Selaiyur, Chennai-600073, IN
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Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2889-2894Abstract
In this study the evaluation of genetic diversity among Ocimum sanctum was undertaken. The method used to find variation among Ocimum sanctum was restriction fragment length polymorphism excluding blot hybridization. The Ocimum sanctum plants were taken from five different regions of Tamil Nadu - Chennai, Karaikudi, Kancheepuram, Vellore, Tanjavur and Puducherry. Along with whole plants the soil and water samples in the regions of the plant was also collected. DNA was isolated using CTAB protocol with ammonium acetate and ethanol precipitation. Then the DNA was quantified and restriction digested with EcoR1 and HindIII (double digested). Double digested Lambda DNA (EcoRI and Hind III) was used as marker and the samples along with marker were electrophoresed in 0.8% agarose gel at 80V and the bands were observed in UV trans illuminator. Based on observation of bands the plants from Karaikudi, Tanjavur, Kancheepuram and Puducherry may have been descended from Chennai plants and Vellore plant may have been descended from Karaikudi plant. There was sufficient variation to predict the migration pattern of the plant. And variation among water-hardness and pH and soil-N, P, K content and pH was also diverse. The measured soil and water characteristics may be few of the reasons for the variation among Ocimum sanctum around Tamil Nadu and Puducherry.Keywords
Ocimum sanctum, CTAB, DNA Extraction, Restriction Digestion, Nutrient of Soil And Hardness.
References
- Nybom H (1990). Application of DNA fingerprinting in plant breeding. Proc. Intern. DNA Fingerprinting Symposium. Bern. pp. 32-45. Lei Y, Gao H, Tsering T, Shi S, Zhong Y (2006). Determination of genetic variation in Rhodiolacrenulata from the Hengduan Mountain Region, China using inter-simple sequence repeats. Genet. Mol. Biol. Sao Paulo (29); 2
- Chen X, Yang J (2004). Species – diversified plant cover enhances orchard ecosystem resistance to climatic stress and soil erosion in subtropical hillside. J. Zhejiang. Uni. Sci. 5(10); 1191-1198.
- Nan P, Shi SH, Peng ST, Tian Cj, Zhong Y (2003). Genetic diversity in Primula obconica (Primulaceae) from central and south-west China as revealed by ISSR markers. Ann. Bot. 91; 329-333.
- Ipek M, Madison L (2001). Genetic Diversity in Garlic (Allium sativum L.) as assessed by AFLPs and Isozymes. American Society for Horticultural Science 98th Annual Conference and Exhibition-22-25, July.
- Muluvi GM, Sprent JI, Soranzo N, Provan J, Odee D, Folkard G, McNicol JW, Provan J, Powell W (1999). Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa. Mol.Ecol. 8; 463-470.
- Cardoso SRS, Eloy NB, Provan J, Cardoso MA, Ferreira PCG (2000). Genetic differentiation of Euterpe edulis Mart populations estimated by AFLP analysis. Mol. Ecol. 9; 1753-1760.
- Katterman FRH, Shattuck VI (1983). An effective method of DNA isolation from the mature leaves of Gossypium species that contain large amounts of phenolics, terpeniodes and tannins. Preparative Biochem, 13: 347-359.
- Edwards K, Johnstone C, Thompson C (1991). A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acid Res. 19: 1349.
- Wang Y, Taylor DE (1993). A rapid CTAB DNA isolation technique useful for RAPD for fingerprinting and other PC Rapplications. Biotechniques 14; 748-750.
- Ziegenhagen B, Scholz F (1998). Methods for difficult plant species. In: Karp A, Issac PG, Ingram DS (eds), Molecular Tools for screening Biodiversity 2.2. Plants and Animals, Chapman and Hall, London. pp.32-35.
- Khanuja SPS, Shasany AK, Darokar MP, Kumar S (1999). Rapid Isolation of DNA from Dry and Fresh Samples of plants producing Large Amounts of Secondary Metabolites and essential oils. Plant Mol. Biol. Rep., 17(1): 74.
- Sambrook J, Fritsch EF, Maniatis T (1989). Molecular cloning. Cold Spring Harbor Laboratory Press, New York.
- Kotaiah, B and Swamy, K.N. 1994. Environmental Engineering Laboratory Manual, Charotar Publishing House, Gujarat, India, 25–26.
- A Study on The Influence of Vermicompost on The Growth Parameters of Arachis hypogaea
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Authors
Affiliations
1 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai-600073, IN
1 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai-600073, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2895-2901Abstract
In nutrition a new era is emerging that is characterized by search for dietary constituents that have benefits beyond those described to the macro and micro-nutrients. The use of chemical fertilizers, contributes largely depletion of fossil fuels, generation of carbon dioxide and contamination of water resource it leads to loss of soil fertility due to imbalance use of fertilizers that has adversely impacted agriculture productivity and causes of soil degradation. Compositing has been recognized as a low cost and environmentally sound process for treatment of many organic wastes. Vermicompost is the type of compositing that was studied on the growth parameters of Ground Nut plant (Arachis hypogaea. L). Vermicompost is a product delivered from the accelerated biological degradation of the organic waste by earth worm and microorganisms. Vermicompost chemical composition contains especially nitrates, exchangeable phosphorous, soluble potassium, calcium and magnesium than that of growth media. The study was mainly aimed on the plant growth and biochemical composition on the seedlings that was grown in difference composition of the vermicompost such as [10%-100%]. The biochemical analysis and enzyme assay shows variations in different concentration.Keywords
Vermicompost, Arachis hypogaea, Fertilizers, Composting.References
- Gupta A, Gopal M. Biological control of pests. 2001, Ed. A. A. Deshmukh, Pub. S. A. Deshmukh, Karad. Ms. India, 101-122.
- Thomas GV, Prabhu SR. Bioinoculants and organic amendments for ensuring crop health in sustainable agriculture.,2001, Ed. A. M. Deshmukh. Pub. S. S. Deshmukh Karad. Ms. India, 122-162.
- Rao MRK, Sathish Kumar M, Jha NK. Comparative yield analysis of Chilli (Capsicum annuum L.) by application of vermicompost and Panchagavya. Journal of Chemical and Pharmaceutical Research, 2015, 7(9):319-323
- Khan A, FouziaIshaq. Chemical nutrient analysis of different composts (Vermicompost and Pitcompost ) and their effect on the growth of a vegetative crop Pisumsativum , Asian Journal of Plant Science and Research, 2011, 1 (1):116-130
- Senthil Kumar D, Satheesh Kumar P, Uthaya Kumar. V, Anbuganapathi G. Influence of Biofertilizer Mixed Flower Waste Vermicompost on the Growth, Yield and Quality of Groundnut (Arachis hypogaea). World Applied Sciences Journal, 2014; 31 (10):1715-1721
- Bindumathi Mohan, Srinivasan TS, Evaluation of organic growth promoters on yield of drylands vegetable crops in India, Journal of Organic Systems, 2008;3(1):26-36
- Hointik HAJ, Bohem MJ. Biocontrol within the context of soil microbial communities: a substrate-dependent phenomenon. Annu Rev Phytopathol, 1999; 37: 427-46
- Bevacqua RF, Mellano VJ. Sewage sludge compost’s cumulative effects on crop growth and soil properties. Compost Sci. Util., 1(3):32
- Norman Q. Arancon, Clive A. Edwards. Effects of vermicompost on plant growth, Paper presented during the International Symposium Workshop on Vermi Technologies for Developing Countries (ISWVT 2005), Los Banos, Philippines, 2005; 6-18
- Arancon NQ, Edwards A, Bablenko A, Cannon J, Galvis P, Metzger D (2008) Influence of vermicomposts; produced by earthworm and microorganisms from castle manure, food waste and paper waste on germination, growth and flowering of petunias in the greenhouse. Appl Soil Ecol, 2008; 39:91–99
- Edwards CA, Arancon NQ. The use of earthworms in the breakdown and management of organic wastes to produce vermicomposts and fees protein. In: Edwards, C.A. (Ed.) Earthworm Ecology, Second Ed. CRC Press, Boca Raton, FL, 2004: 345-379
- Krishnamoorthy RV, Vajrabhiah SN. Biological activity of earthworm casts: an assessment of plant growth promoter levels in casts. Proc Indian Acad Sci, 1986; 95:341–351
- Tomati U, Grappelli A, Galli E. The hormone like effect of earthworm casts on plant growth. Biol Fertil Soils, 1988; 5:288–294
- Mathiavanan S, Kalaikandhan R, Chidambaram ALA, Sundramoorthy P. Effect of Vermicompost on the growth and nutrient status in groundnut (Arachis hypogaea. L). Asian Journal of Plant Sciences and Research. 2013; 3(2):15-22
- Atiyeh RM, Subler S, Edwards CA., Bachman G, Metzger JD, Shuster W. Effect of vermicompost and compost on plant growth of the horticultural container media and soil, Pedobiologia, 2000; 44:579–590
- Rakesh Joshi, Adarsh Pal Vig, Effect of Vermicompost on Growth, Yield and Quality of Tomato (Lycopersicumesculentum L), African Journal of Basic & Applied Sciences, 2010; 2 (3-4):117-123
- C., Arnold. J, Tato. A,. Zaller JG, Domínguez. J, Compost and vermicompost as nursery pot components: effects on tomato plant growth and morphology, Spanish Journal of Agricultural Research 2009,7(4), 944-951
- Ravi MT, Lenin. M, Selvakumar G, Thangadurai R, Regular Article Growth and Nutrient Content Variation of Groundnut (Arachis hypogaea L.) under Vermicompost Application, Journal of Experimental Sciences, 1010; l (1): 12-16
- Abdul-Baki, AA, Anderson J. Vigour detrmination of Soybean seed by multiple criteria. Crop Sci., 1973, 3:630-633.
- O.H.N.J. Rosenbrough. A. L. Far and R. J Randall, 1951. Protein measurement with the folin phenol reagent, J.Biol.chem.,193:265-275
- Bioremediation of Nitrate Reduction Present in Leather Industries Effluent by using Marine Algae
Abstract Views :285 |
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Authors
Affiliations
1 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, 173, Agaram Main Road, Selaiyur, Chennai-600 0073, Tamil Nadu, IN
1 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, 173, Agaram Main Road, Selaiyur, Chennai-600 0073, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3522-3526Abstract
Nitrogen occurs naturally under oxidation of nitric anhydride, N2O5 and nitrates, NO3- and also from ammonia, and ammonium ions, NH4+. Because of increase in human activities environmental nitrate concentrations also increased which leads to introduction of large quantities of nitrates into ground and surface waters. Agriculture is the major source of nitrate pollution because of using of nitrogen-containing fertilizers, concentrated livestock and poultry farming. Nitrates are also released from the industrial production of glass, explosives, as well as from various chemical production and separation processes. Highest reduction in nitrate content was found in benzene extract of U.lactuca 91% and very minimal reduction of 12 % was found in aqueous extract of E.intestinalis. Among all, the extracts of Ulva lactuca, Enteromorpha flexuosa fell under Category A (Good reduction), and benzene was found to be the best solvent system. Maximum reduction by powdered algae was found in C.antenina collected from Covelong and Puduchery (375 mg/l).Keywords
Nitrate, Reduction, Bioremediation, Leather Effluent, Seaweed.References
- EPA (Environmental Protection Agency), Public health goals for nitrate and nitrite in drinking water, Pesticide and Environmental Toxicology Section, Office of Environmental Health Hazard Assessment, California. 1997.
- Forman D. Nitrate exposure and human cancer. In: Bogardi, I., Kuzelka, R. (Eds.), Nitrate Contamination, RD NATO ASI Series. 1991; vol. G 30. Springer-Verlag.
- Kapoora A, Viraraghavana T and Cullimoreb DR. Removal of heavy metals using the fungus Aspergillus niger, Bioresource Technology. 1999; 70: 95-103.
- Englehardt D, De JD and Kalu EE. Electroreduction of nitrate and nitrite ion on a platinum-group-metal catalyst-modified carbon fiber electrodechrono amperometry and mechanism studies. J. Electrochem. Soc. 2000; 147: 4573–4579.
- Murphy AP. Chemical removal of nitrate from water. Nature. 1991; 350: 223-225.
- Salem K, Sandeaux J, Molenat J, Sandeaux R and Gavach C. Elimination of nitrate from drinking water by using electrochemical processes. Desalination. 1995; 101: 123-131.
- Debi Prasad Pattanaik, Sanjeev Mishra, Anshuman Mishra, Sharmila S, Dhanalakshmi V, Anbuselvi S, and Jeyanthi Rebecca L. Phytoremediation of Mercury, Aluminium and Chromium using Raphanus sativus and Zea mays. International Journal of Biotechnology and Bioengineering Research. 2011; 2 (2): 277–286.
- Kowsalya E, Sharmila S and Jeyanthi Rebbecca L. Removal of congo red dye from effluent sample using casurina leaves as a adsorbent. Journal of Chemical and Pharmaceutical Research. 2015; 7(9): 659-665.
- SAP (Standard Analytical procedure), Hydrology Project, Govt. of India & Govt. of Netherlands.1999.
- Ghafari S, Hasan M and Aroua MK. Bio-electrochemical removal of nitrate from water and wastewater – a review. Bioresour. Technol. 2008; 99: 3965–3974.
- Gupta SK, Gupta AB, Gupta RC, Seth AK, Bassain JK and Gupta A. Recurrent acute respiratory tract infections in areas with high nitrate concentrations in drinking water. Environ. Health Perspect. 2000; 108 (4): 363–365.
- Fernandez-Nava Y, Maranon E, Soons J and Castrillón L. Denitrification of wastewater containing high nitrate and calcium concentrations. Bioresour. Technol. 2008; 99: 7976–7981.
- Virkutyte J and Jegatheesan V. Electro-Fenton, hydrogenotrophic and Fe2+ ions mediated TOC and nitrate removal from aquaculture system: different experimental strategies. Bioresour. Technol. 2009, 100: 2189–2197.
- Wang Q, Feng C, Zhao Y and Hao C, Denitrification of nitrate contaminated groundwater with a fiber-based biofilm reactor. Bioresour. Technol. 2009, 100: 2222–2227.
- Samatya S, Kabay N, Yüksel ü, Rda M and Yüksel M, Removal of nitrate from aqueous solution by nitrate selective ion exchange resins. React. Funct. Polym. 2006; 6 (11): 1206–1214.
- Petri OA and Safonova TY. Electroreduction of nitrate and nitrite anions on platinum metals: a model process for elucidating the nature of the passivation by hydrogen adsorption. J. Electroanal. Chem. 1992; 331: 897–912.
- Ureta-Zanartu S and Yanez C. Electroreduction of nitrate ion on Pt, Ir and on 70:30 Pt: Ir alloy. Electrochim. Acta. 1997; 42: 1725–1731.
- Duarte HA, Jha K and Weidner JW. Electrochemical reduction of nitrates and nitrites in alkaline media in the presence of hexavalent chromium. J. Appl. Electrochem. 1998; 28: 811–817.
- Huang CP, Wang HW and Chiu PC. Nitrate reduction by metallic iron. Water Res. 1998; 32: 2257–2264.
- Vooys ACA, Santen RA and Veen JAR. Electrocatalytic reduction of NO_ 3 on palladium/copper electrodes. J. Mol. Catal. A Chem. 2000; 154: 203–215.
- Wang Y, Qu J, Wu R and Lei P. The electrocatalytic reduction of nitrate in water on Pd/Sn-modified activated carbon fiber electrode. Water Res. 2006; 40: 1224–1232.
- Li M, Feng CP, Zhang ZY and Sugiura N. Efficient electrochemical reduction of nitrate to nitrogen using Ti/IrO2–Pt anode and different cathodes. Electrochim. Acta. 2009; 54: 4600–4606.
- Kesseru P, Kiss I, Bihari Z and Polya´k B. Biological denitrification in a continuous-flow pilot bioreactor containing immobilized Pseudomonas butanovora cells. Bioresour. Technol. 2003; 87: 75–80.
- Dhamole PB, Nair RR, D’Souza SF and Lele SS. Simultaneous removal of carbon and nitrate in an airlift bioreactor. Bioresour. Technol. 2009; 100: 1082–1086.
- Lei X and Maekawa T. Electrochemical treatment of anaerobic digestion effluent using a Ti/Pt–IrO2 electrode. Bioresour. Technol. 2007; 98: 3521–3525.
- Amaraselvam K, Sharmila S and Jeyanthi Rebecca L. Removal of colour from textile industry effluent using seaweed. International Journal of Pharmacy & Technology. 2016; 8(3): 15740-15747.
- Metcalf and Eddy. Waste water engineering, treatment, disposal, reuse, New York: McGraw-Hill. 1979.