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Sharmila, D.
- Evaluation of Genetic Diversity among Ocimum sanctum
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Authors
Affiliations
1 Department of Industrial Biotechnology, BIHER, Selaiyur, Chennai-600073, IN
1 Department of Industrial Biotechnology, BIHER, Selaiyur, Chennai-600073, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2889-2894Abstract
In this study the evaluation of genetic diversity among Ocimum sanctum was undertaken. The method used to find variation among Ocimum sanctum was restriction fragment length polymorphism excluding blot hybridization. The Ocimum sanctum plants were taken from five different regions of Tamil Nadu - Chennai, Karaikudi, Kancheepuram, Vellore, Tanjavur and Puducherry. Along with whole plants the soil and water samples in the regions of the plant was also collected. DNA was isolated using CTAB protocol with ammonium acetate and ethanol precipitation. Then the DNA was quantified and restriction digested with EcoR1 and HindIII (double digested). Double digested Lambda DNA (EcoRI and Hind III) was used as marker and the samples along with marker were electrophoresed in 0.8% agarose gel at 80V and the bands were observed in UV trans illuminator. Based on observation of bands the plants from Karaikudi, Tanjavur, Kancheepuram and Puducherry may have been descended from Chennai plants and Vellore plant may have been descended from Karaikudi plant. There was sufficient variation to predict the migration pattern of the plant. And variation among water-hardness and pH and soil-N, P, K content and pH was also diverse. The measured soil and water characteristics may be few of the reasons for the variation among Ocimum sanctum around Tamil Nadu and Puducherry.Keywords
Ocimum sanctum, CTAB, DNA Extraction, Restriction Digestion, Nutrient of Soil And Hardness.
References
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- A Study on The Influence of Vermicompost on The Growth Parameters of Arachis hypogaea
Abstract Views :167 |
PDF Views:0
Authors
Affiliations
1 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai-600073, IN
1 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai-600073, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2895-2901Abstract
In nutrition a new era is emerging that is characterized by search for dietary constituents that have benefits beyond those described to the macro and micro-nutrients. The use of chemical fertilizers, contributes largely depletion of fossil fuels, generation of carbon dioxide and contamination of water resource it leads to loss of soil fertility due to imbalance use of fertilizers that has adversely impacted agriculture productivity and causes of soil degradation. Compositing has been recognized as a low cost and environmentally sound process for treatment of many organic wastes. Vermicompost is the type of compositing that was studied on the growth parameters of Ground Nut plant (Arachis hypogaea. L). Vermicompost is a product delivered from the accelerated biological degradation of the organic waste by earth worm and microorganisms. Vermicompost chemical composition contains especially nitrates, exchangeable phosphorous, soluble potassium, calcium and magnesium than that of growth media. The study was mainly aimed on the plant growth and biochemical composition on the seedlings that was grown in difference composition of the vermicompost such as [10%-100%]. The biochemical analysis and enzyme assay shows variations in different concentration.Keywords
Vermicompost, Arachis hypogaea, Fertilizers, Composting.References
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