Research Journal of Pharmacy and Technology

Ashirbad Nanda ¹, Sai Aparna ², Rudra Narayan Sahoo ³, Arunima Pramanik ¹, Bhargab Satpathy ⁴, Subrata Mallick ¹, Debajyoti Das ¹, Manorama Patri ²

Affiliations
1 School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha 751003,, IN
2 Neurobiology Laboratory, Department of Zoology, School of Life Sciences, Ravenshaw University, College Square, Cuttack, Odisha 753003,, IN
3 Centurion University of Technology and Management, Odisha, IN
4 Research Associate II Hetero Biopharm Unit III, Hyderabad, Telangana., IN

Abstract

Proliferative retinopathy is described as a progressive neovascularization in the inner surface of the retina or vitreous, which subsequently threatens vision by causing retinal detachment or vitreous hemorrhage. Reactive oxygen species generation and its related vasoproliferative factor up regulation play a major role in progression of the disease. The present study focuses on Carr induced reactive species generation with its related vasoproliferative factor up regulation and ameliorative activity of AML. Biochemical estimation of catalase, lipid peroxidation, hypoxia inducing factor (HIF-1α) and guanyl-s-transferase (GST) were done. AML treated group showed significant increase in catalase enzyme, guanyl-s-transferase and significantly reduced the lipid peroxidation. Western blotting assay showed a significant decrease in HIF-1α in AML treated group. The progression of PR alters the endogenous cell signalling thus causing havoc in retinal cells. Therefore, it could be hypothesized that use of AML can be a new detection method for therapeutic strategies to prevent blindness in PR.

Keywords

Amlodipine, Proliferative retinopathy, Biochemical estimation, Hypoxia.

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