Research Journal of Pharmacy and Technology

Amaq Fadholly ¹, Arif N. M. Ansori ¹, Shara Jayanti ¹, Annise Proboningrat ¹, Muhammad K. J. Kusala ¹, Naimah Putri ¹, Fedik A. Rantam ¹, Sri A. Sudjarwo ¹

Affiliations
1 Universitas Airlangga, Surabaya, 60115, ID

Abstract

The extract of Allium cepa L. is commonly used as adjuvant therapy for cancer that includes a potential source of anticancer properties. This study aimed to evaluate the cytotoxicity from a crude extract of Allium cepa L. against human colon cancer (WiDr) cells. The cytotoxic effect of Allium cepa L. extract was determined using MTT assay. The Allium cepa L. extract inhibited the proliferation of WiDr cells, but revealed no potent cytotoxicity with IC_<50 value of 1363.29 μg/mL on WiDr cells. The results showed that Allium cepa L. extract was no longer active as an anticancer drug, but it would be more active using the direct-bioactive compound of Allium cepa L. Some intens observations are still required to maximize its potential as one of chemotherapeutic agents in the future. On the other hand, further studies are needed to obtain the right dose of Allium cepa L. extract that can inhibit the cell growth well and determine the accuracy of its mechanism on WiDr cells as one of therapeutic strategies to treat human colon cancer.

Keywords

Allium cepa L, WiDr Cells, Cytotoxic.

Full Text

     

Annise Proboningrat ¹, Viol Dhea Kharisma ², Arif Nur Muhammad Ansori ¹, Rinza Rahmawati ³, Amaq Fadholly ¹, Gabrielle Ann Villar Posa ⁴, Sri Agus Sudjarwo ⁵, Fedik Abdul Rantam ⁶, Agung Budianto Achmad ⁷

Affiliations
1 Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, 60115, Surabaya,, ID
2 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, 65145, Malang,, ID
3 Department of Chemistry, Faculty of Health Sciences, Muhammadiyah University of Surabaya, 60113, Surabaya,, ID
4 School of Environmental Science and Management, University of the Philippines Los Baños, Los Baños,, PH
5 Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga, 60115, Surabaya,, ID
6 Research Center for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, 60115, Surabaya,, ID
7 Department of Health, Faculty of Vocational Studies, Universitas Airlangga, 60115, Surabaya,, ID

Abstract

Globally, the leading cause of death from cancer in women is infection with the human papillomavirus (HPV). This calls for imperative actions to explore anticancer drugs against this threatening viral infection, in which case, natural ingredients are presumed to be a promising source. Several studies show that plant-origin compounds such as allicin, apigenin, capsaicin, cyanidin, fisetin, genistein, laricitrin, naringenin, piperine, and syringetin have demonstrated therapeutic effects against several cancer types. In this study, the interaction mechanism of these compounds with HPV-18 E6 oncoprotein, that is known to downregulate tumor suppressor p53, was predicted using an in silico approach. Molecular docking simulations of natural ligands and E6 protein were performe, followed by chemical interaction analysis and 3D molecular visualization. Results indicated that fisetin is the best natural inhibitor as it has the lowest binding energy. It is highly recommended that the results of this study be used as a reference in designing anticancer drugs in vitro and in vivo.

Keywords

HPV, E6, cervical cancer, inhibitors, virtual screening.

Full Text