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Analysis of Phytochemical Compounds Present in Some Traditional Anti-dermatophytic Plants by Thin Layer, Column Chromatographic Methods


Affiliations
1 PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
2 School of Biosciences, Faculty of Medicine, Bioscience and Nursing, MAHSA University, Selangor,, Malaysia
     

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Traditionally, various medicinal plants were known to possess anti-dermatophytic potential and have been used for many years to treat skin diseases. We are aimed to examine anti-dermatophytic plants like Calotropis gigantea (L.) R. Br., Cassia alata (L) Roxb., Kaempferia galanga L., Melia azedarach L. and Sesbania grandiflora (L) Pers. for the quantification and separation of phyto-chemical compounds through thin layer and column chromatography. Five plant samples comprising of leaves, stem fiber and rhizome were separately extracted using five solvents such as, ethanol, methanol, acetone, chloroform and petroleum ether. The solvent extracts were subsequently investigated for qualitative, quantitative phytochemical, thin layer and column chromatographic analysis using standard assays. The experimental findings revealed the varied presence of compounds such as sugar, amino acid, protein, phenol, alkaloid and flavonoid in the solvent extracts used. In TLC profiling, a number of phyto chemical compounds, at different ranges of visible spots, with different Rf values at varying solvent system, were identified. Also, column fractions at different solvent run ratio (hexane: acetone = 8:2, 6:4, 4:6, 2:8) were identified with a wide range of color variations at different retention time and were collected sequentially. The present analysis exhibit a significant role to identify and differentiate therapeutic phyto-compounds which helps for further study on isolation and characterization with anti-dermatophytic potential.

Keywords

Anti-dermatophytic, Phytochemical compounds, Thin layer, Column, Chromatography.
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  • Kavitha Sagar and Vidyasagar G.M. Anti-dermatophytic activity of some traditionally used medicinal plants of North Karnataka Region. Journal of Applied Pharmaceutical Science. 2013; 3(2): 077-083.

  • Pierangeli, G., Vital, G., and Rivera, W. Antimicrobial activity and cytotoxicity of Chromolaena odorata (L.f) King and Robinson and Uncaria perrottetii (A. Rich) Merr Extracts. Journal of Medicinal plants Research. 2009; 3(7): 511-518.

  • Savithramma, N., Linga Rao, M., Beenaprabha.. Phytochemical studies of Dysophylla myosuroides (Roth.) Benth. In. wall. And Talinum cuneifolium (Vahl.) Willd. Research Journal of Phytochemicals. 2011; 5(3): 163-169.

  • Obeidat, M., M. Shatnawi, M. Al-Alawi, E. Al-Zubi and H. Al-Dmoor.. Antimicrobial activity of crude extracts of some plant leaves. Research Journal of Microbiology. 2012; 7: 59-67.

  • De-fathima etal., 2006 de Fatima, A.; Modolo, L. V.; Conegero, L. S.; Pilli, R. A.; Ferreira, C. V.; Kohn, L. K.; de Carvalho, J. E. Styryl Lactones and Their Derivatives: Biological Activities, Mechanisms of Action and Potential Leads for Drug Design. Current Medicnal Chemistry 2006; 13(28): 3371-3384.

  • Geetha TS, Geetha N. Phytochemical screening, quantitative of primary and secondary metabolites of Cymbopogan citratus (DC) Stapf. Leaves from Kodaikanal hills. International Journal of Pharmaceutical Technology and Research. 2014; 6(2): 521-529.

  • Vyas, G.D. Soil Fertility Deterioration in cropland Due to Pesticide. Journal of Indian Botanical Society. 1999; 78: 177-178.

  • Kaushik, J.C., Arya Sanjay, Triathi, N.N., and Arya, S. Antifungal properties of some plant extracts against the damping off fungi of forest nurseries. Indian Journal of Forestry. 2002; 25: 359-361.

  • Chaman Lal and Verma, L.R.. Use of certain bio-products for insect-pest control. Indian Journal of Traditional Knowledge. 2006; 5 (1): 79-82.

  • Okwu, D.E. Improving the nutrition value of Cassava tapioca meal with local species. Nutraceutical, Functional and Medicinal food. 2001; 3: 43-51.

  • Okwu, D.E. Evaluation of the chemical composition of indigenous species and flavouring agents. Global Journal of Pure and applied Sciene 2001; 8: 455-459.

  • Ravindra Sharma. Agrotechniques in medicinal plants. Daya Publishing house, New Delhi. 2004; 1(2): 197-199.

  • Han, X., Shen, T., Lou, H. Dietary polyphenols and their biological significance. International Journal of Molecular Sciences. 2007; 8: 950-988.

  • Balch, J.F., Balch, P.A. Prescription for Nutritional Healing. New York. Avary, penguin Putnam Inc., 2000; pp.267-270

  • Linuma, M., Tsuchiya, H., Salo, M., Yokoyama, J., Ohyama, M., Ohkawa,Y. Flavanones with potent antibacterial activity against methicillin-resistant Staphylococcus aureus. Journal of Pharmaceutics and Pharmacology 1994; 46(11): 892-895.

  • Gupta, V.K., Singh, G.D., Singh,S., Kaul, A. Medicinal Plants: Phytochemistry, Pharmacolgoy and Therapeutics. 2010 Daya Publishing House, Delhi.

  • Archana, P., Samatha, T., Mahitha, B., Chamundeswari and RamaSwamy, N. Preliminary phytochemical screening from leaf and seed extracts of Senna alata L. Roxb-an Ethnomedicinalplant. International Journal of Pharmaceutical and Biological Research 2012; 3(3): 82-89.

  • Acharya TK, Chatterjee IB. Isolation of chrysophanic acid-9- anthrone, the major antifungal principle of Cassia tora. Lloydia. 1975; 38: 218-20.

  • Kader HAA, Seddek SR, El-Shanawany AA. In vitro study of the effect of some medicinal plants on the growth of some dermatophytes. Assiut Veterinary Medical Journal. 1989; 34: 36-42.

  • Onawunmi GO. Evaluation of the antifungal activity of Lemon grass oil. International Journal of Crude Drug Research. 1989; 27: 121-26.

  • Singh S, Singh SK, Tripathi SC. Fungitoxic properties of essential oil of Eucalyptus rostrata. Indian Perfumer. 1988; 32: 190-93.

  • Gamble, J.S. Flora of the Presidency of Madras. BSI, Calcutta. 1967; 1: 227--286.

  • Shripad M. Bairagi, Prashant Ghuze, Ritu Gilhotra. Pharmacology of Natural products: A recent approach on Calotropis gigantea and Calotropis procera. Ars harm. 2018; 59(1): 37-44

  • Evans WC. Trease and Evans’. Pharmacognosy. 2005; 41-47.

  • Verma VN. The chemical study of Calotropis. International Letters of Chemistry, Physics and Astronomy. 2014; 1: 74-90.10.18052/www.scipress.com/ILCPA.20.74.

  • Palanichamy S and Nagarajan S. Antifungal activity of Cassia alata leaf extract. Journal of Ethnopharmacology 1990; 29(3): 337-340.

  • Iyengar MA, Pattabhi Rama Rao, Bairy M, Kamath MS. Antimicrobial activity of essential oil of Curcuma longa leaves. Indian Drugs 1995; 32(6): 249-250.

  • Hasan CM, Islam SN, Begum K, Ilias M, Husian A. Antibacterial activities of the leaves and stem bark of Cassia alata L. Bangladesh Journal of Botany.1989; 17(2): 135-139.

  • Ibrahim D, Osman H. Antimicrobial activity of Cassia alata from Malaysia. Journal of Ethnopharmacology 1995; 45(3): 151-6.

  • Ogunti EOA, Aladesanmi AJ, Adesanya SA. Antimicrobial activity of Cassia alata. Fitoterapia 1991; 62(2): 537-539.

  • Palanichamy SE, Amal Bhaskar and Nagarajan S. Antibacterial activity of Cassia alata. Fitoterapia 1991; 62(3): 249-252.

  • Sakharkar BM. Principles of Hospital Administration and Planning, 1999. Jaypee Brothers Medical Publishers (P) Ltd., New Delhi. Edn.

  • Abatan MOA. Note of the anti-inflammatory action of plants of some Cassia species. Fitoterapia 1990; 61(4): 336-338.

  • Palanichamy S, Nagarajan S, Devasagayam. Effect of Cassia alata leaf extract on hyperglycemic rats. Journal of. Ethnopharmacology. 1988; 22(1): 81-90. 35. Ogunti EO, Elujobi AA. Laxative activity of Cassia alata. Fitoterapia. 1993; 64: 437-439.

  • Palanichamy S, Amala Bhaskar E, Bakthavasalam R, Nagarajan S. Wound Healing activity of Cassia alata. Fitoterapia. 1991; 62(2): 153-156.

  • Kirtikar KR, Basu BD. Indian Medicinal Plants, Volume IV. In: Blatter E, Caius JF, Mahaskar KS (eds.), Lalit Mohan Basu, Allahabad, India, 1996: 2422-2423.

  • Vedha pal JS, Ramya N, Senthilkumaran K, Senthilnathan B, Murugan N. Phytochemical and qualitative analysis of leaves of Melia azaderach and seeds of Piper longum. Journal of Chemistry and Pharmaceutical Research. 2018; 10(2): 25-31.

  • Venkateshwarlu G, Shantha TR, Siddamallaya N, Kishore KR. Traditional and ayurvedic medicinal importance of Agasthya leaves [Sesbania grandiflora (L) Pers.]W.R.T its pharmagonostic and physiochemical evaluation. International Journal of Research in Ayurveda and Pharmacy 2012; 3(2): 192-198.

  • Kokate, C.K. Practical Pharmacognosy. 1999. 4th edn. Vallabh Prakashan Publication, New Delhi. India. pp.107-121

  • Evans, W. C. Trease and Evans Pharmacognosy, 2000. 15th edition. W.B Sauders Company Ltd, London. Pp.137-139, 230-240.

  • Mace, M.E. Histochemical localization of phenols in healthy and diseased banana roots. Physiol. Plantarum. 1963; 16: 915-925.

  • Harbone,J.B.. Phytochemical methods: A guide to modern techniques of plant analysis. 1973. 3rd Edition. Chapman and Hall Ltd, London. pp. 49-188.

  • Trease GE, Evans WC. A Text book of Pharmcognosy, 1983. 6th Edition. Bailliere Tindall, London: 795.

  • Dubios, M., Gilles, K.A., Hamilton, J.K., Roberts, P. A. and Smith, F. A colorimetric method for the determination of sugars. Nature. 1956; 168-167.

  • Moore, S. and Stein, W.H. Photometric ninhydrin methods for use in the chromatography of amino acids. Journal of Biological. Chemistry 1948; 176: 367 -388.

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.j. Protein measurement with the Folin-Phenol reagent. Journal of Biochemistry. 1951; 193(1) : 265-75

  • Mahadevan, A. and Sridhar, R. Method in physiological plant pathology. 1982. Sivakami publications, Chennai.


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  • Analysis of Phytochemical Compounds Present in Some Traditional Anti-dermatophytic Plants by Thin Layer, Column Chromatographic Methods

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Authors

Sownthariya C
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
Shanthi P
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
Balavinayagamani Ganapathy
School of Biosciences, Faculty of Medicine, Bioscience and Nursing, MAHSA University, Selangor,, Malaysia
Priyadharshni K
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
Thiripura Sundari U
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
Nagaraja Suryadevara
School of Biosciences, Faculty of Medicine, Bioscience and Nursing, MAHSA University, Selangor,, Malaysia

Abstract


Traditionally, various medicinal plants were known to possess anti-dermatophytic potential and have been used for many years to treat skin diseases. We are aimed to examine anti-dermatophytic plants like Calotropis gigantea (L.) R. Br., Cassia alata (L) Roxb., Kaempferia galanga L., Melia azedarach L. and Sesbania grandiflora (L) Pers. for the quantification and separation of phyto-chemical compounds through thin layer and column chromatography. Five plant samples comprising of leaves, stem fiber and rhizome were separately extracted using five solvents such as, ethanol, methanol, acetone, chloroform and petroleum ether. The solvent extracts were subsequently investigated for qualitative, quantitative phytochemical, thin layer and column chromatographic analysis using standard assays. The experimental findings revealed the varied presence of compounds such as sugar, amino acid, protein, phenol, alkaloid and flavonoid in the solvent extracts used. In TLC profiling, a number of phyto chemical compounds, at different ranges of visible spots, with different Rf values at varying solvent system, were identified. Also, column fractions at different solvent run ratio (hexane: acetone = 8:2, 6:4, 4:6, 2:8) were identified with a wide range of color variations at different retention time and were collected sequentially. The present analysis exhibit a significant role to identify and differentiate therapeutic phyto-compounds which helps for further study on isolation and characterization with anti-dermatophytic potential.

Keywords


Anti-dermatophytic, Phytochemical compounds, Thin layer, Column, Chromatography.

References