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Antioxidant and Antibacterial Potential of Lycium Shawii Roem. And Schult (Awsaj) and Tetraena Qatarensis (Hadidi) Beier and Thulin Leaves: A Herbal Approach to Combat Drug Resistance and Oxidative Stress


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1 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra, Saudi Arabia
     

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The present investigation deals with the antioxidant and antibacterial activity of chloroform and methanolic extracts of Lycium shawii and Tetraena qatarensis leaves. Antioxidant activity was evaluated using DPPH and FRAP assay. In the DPPH assay, the lowest IC50 values 45.63 and 46.17μg/ml were observed with methanolic and chloroform extract Lycium shawii leaves, respectively. In FRAP assay most of the extracts showed optical density >0.5 at 40μg/ml. In the antibacterial activity test, all tested extracts were found active towards all bacterial strains with MIC ranged between 0.03125 to 0.12mg/mL. Subsequently, in the combined effect assay, the highest fold increase (92.31%) was observed against Salmonella typhi with methanol extract of Lycium shawii leaves. The examined plants could be used as a treatment for microbial infection and oxidative stress after detailed follow-up investigations.

Keywords

Lycium shawii, Tetraena qatarensis, DPPH, FRAP, Antibacterial activity.
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  • Freeman CD, Klutman NE and Lamp KC. Metronidazole: A therapeutic review and update. Drugs 1997 Nov. 54: 679-708. doi: 10.2165/00003495-199754050-00003.

  • Weir M, Rajić A, Dutil L, Uhland C and Bruneau N. Zoonotic bacteria and antimicrobial resistance in aquaculture: opportunities for surveillance in Canada. Can. Vet. J., 53: 619-622. Weir M, Rajić A, Dutil L, Uhland C, Bruneau N. Zoonotic bacteria and antimicrobial resistance in aquaculture: opportunities for surveillance in Canada. Canadian Veternary Journal 2012 Jun;53(6):619-22.PMCID: PMC3354819.

  • Padmini E, Valarmathi A and Rani MU. Comparative analysis of chemical composition and antibacterial activities of Mentha spicata and Camellia sinensis. Asian Journal of Experimental Biology amd Science 2010; 1: 772-781. Corpus ID: 8809692.

  • Calzada F, Yépez-mulia L and Tapia-contreras A. Effect of Mexican medicinal plant used to treat trichomoniasis on Trichomonas vaginalis trophozoites. Jourbnal of Ethnopharmacol 2007 Jun7; 113: 248-251. Doı: 10.1016/j.jep.2007.06.001

  • Yang J, Liu RH and Halim L. Antioxidant and antiproliferative activities of common edible nut seeds. LWT - Food Sciences and Technology 2009; 42: 1-8. Doi: 10.1016/j.lwt.2008.07.007

  • Aderinboye RY, Oladeji OT, Abaire MA, Sobayo RA, Oso AO, Oni AO, Yusuf KO, Osho SO and Bamgbose AM. Performance of weaner rabbits fed a concentrate diet supplemented with pawpaw leaves. Trop. Anım. Health Pro 2015; 47: 323-329.

  • Halliwell B. Antioxidants and human disease: a general introduction. Nutr. Rev 1997Jan, 55: S44-S49. Doı: 10.1111/j.1753-4887.1997.tb06100.x

  • Parmar J, Sharma P, Verma P and Goyal PK. Chemopreventive action of Syzygium cumini on DMBA-induced skin papillomagenesis in mice. Asian Pacific Journal of Cancer Preview 2010;11: 261-266. PMID: 20593968.

  • Kohen R and Nyska A. Oxidation of biological systems: oxidativestress phenomena, antioxidants, redox reactions, and methods of their quantification. Toxicological Pathology 2002 Dec; 30: 620-650. Doı: 10.1080/01926230290166724

  • Kumar A and Kumar D. Development of antioxidant rich fruit supplemented probiotic yogurts using free and microencapsulated Lactobacillus rhamnosus culture. Journal of Food Science and Technology 2016 Jan; 53: 667-675. Doi: 10.1007/s13197-015-1997-7

  • Mariyappan, M., Bharathidasan, R., Mahalingam, R., Madhanraj, P., Panneerselvam, A., and Ambikapathy, V. Antibacterial Activity of Cardiospermum halicacabum and Melothria heterophylla. Asian Journal of Pharmaceutical Research 2011; 1(4), 111-113. DOI: 10.5958/2231–5691

  • Paul, S., and Saha, D. Comparative Study of the Efficacy of Barleria prionitis Leaf extracts against Bacteria. Asian Journal of Pharmaceutical Research 2012; 2(3), 107-110. DOI: 10.5958/2231–5691

  • Valli, G., and Jeyalakshmi, M. Preliminary phytochemical and antioxidant study of Odina woodier leaf extract. Asian Journal of Pharmaceutical Research 2012; 2(4), 153-155. DOI: 10.5958/2231–5691 .

  • Madhu, C., Swapna, J., Neelima, K., and Shah, M. V. A comparative evaluation of the antioxidant activity of some medicinal plants popularly used in India. Asian Journal of Research in Pharmaceutical Science 2012; 2(3), 98-100. DOI: 10.3923/ajps.2020.127.132.

  • Malathi, R., John, S. A., and Cholarajan, A. Antioxidant activity of extract from the leaves of Tylophora asthmatica. Journal of Microbiology and Antimicrobials 2012; 4(4), 70-73. DOI: 10.5897/JMA11.104.

  • Kamleshiya, P., Meshram, V. G., and Ansari, A. H. The in-vitro Bacteriostatic Potential of Some Traditionally used Indian Spices. Asian Journal of Research in Chemistry 2012; 5(4), 492-496. DOI: 10.5958/0974-4150

  • Jaiganesh, K. P., Sreedharren, B., Arunachalam, G., Nirmala, R., and Nepolean, R. Pharmacognostical and Antimicrobial investigation of Jatropha curcas, Linn., Leaf. Asian Journal of Research in Pharmaceutical Science 2013; 3(4), 195-199. DOI: 10.52711/2231-5659

  • Jadhav, G. B., and Saudagar, R. B. (2014). Free radical scavenging and antioxidant activity of Punica granatum Linn. Asian Journal of Research in Pharmaceutical Science 2014, 4(2), 51-54. DOI: 10.52711/0974-360X.2021.00363

  • Rej, S., Dutta, M., Jamal, S., Das, S., and Chatterjee, S. Study of phytochemical constituents and antibacterial activity of Clerodendrum infortunatum. Asian Journal of Research in Pharmaceutical Science 2014; 4(4), 187-195. DOI: 10.52711/2231-5659

  • Rammo, R. N. Bactericidal and anti-biofilm formation of aqueous plant extracts against pathogenic bacteria. Asian Journal of Pharmaceutical Research 2017; 7(1), 25-29. DOI: 10.5958/2231–5691

  • Dahech I, Farah W, Trigui M, et al. Antioxidant and antimicrobial activities of Lycium shawii fruits extract. International Journal of Biological Macromolecule 2013. Jun1;60:328-333. Doi: 10.1016/j.ijbiomac.2013.05.020.

  • Phondani PC, Bhatt A, Elsarrag E, Horr YA. Ethnobotanical magnitude towards sustainable utilization of wild foliage in Arabian Desert. Journal of Traditional Complement Medicine 2016. July3;6:209-218. doi: https://doi.org/10.1016/j.jtcme.2015.03.003

  • Ksouri, R., Ksouri, W. M., Jallali, I., Debez, A., Magné, C., Hiroko, I., and Abdelly, C. Medicinal halophytes: Potent source of health promoting biomolecules with medical, nutraceutical and food applications. Critical Reviews Biotechnology 2012 Dec; 32, 289–326. doi: 10.3109/07388551.2011.630647.

  • Lopes, A., Rodrigues, M. J., Pereira, C., Oliveira, M., Barreira, L., Varela, J., Custódio, L. Natural products from extreme marine environments: Searching for potential industrial uses within extremophile plants. Industrial Crops Products 2016; 94, 299–307.

  • Oyaizu M. Studies on products of browning reaction: antioxidative activity of products of browning reaction. Japanese Journal of Nutrition and Diet 1986; 44: 307-315. Doi: https://doi.org/10.5264/eiyogakuzashi.44.307.

  • Naznin A and Hasan N. In vitro antioxidant activity of methanolic leaves and flowers extracts of Lippia alba. Int. J. Res.Med. Sci 2009; 4: 107-110.

  • National Committee for Clinical Laboratory Standards. Performance standard for antimicrobial disc susceptibility test, Approved Standard. NCCL Publication, Villanova, USA.1993.

  • Chemical Laboratory Standards Institute (2006). Methods for dilution antimicrobial susceptibility tests for Bacteria that grow aerobically; Approved Standard, Seventh ed., CLSI Document M7- A7, Wayne, Pennsylvania, USA.

  • Thangapandiyan, S., and Prema, P. Chemically fabricated silver nanoparticles enhances the activity of antibiotics against selected human bacterial pathogens. International Journal of Pharmaceutical Sciences and Research 2012; 3(5), 1415-1422. DOI: http://dx.doi.org/10.13040/IJPSR.0975-8232.

  • Ali, S. S., El-Zawawy, N. A., Al-Tohamy, R., El-Sapagh, S., Mustafa, A. M., and Sun, J. Lycium shawii Roem. and Schult.: A new bioactive antimicrobial and antioxidant agent to combat multi-drug/pan-drug resistant pathogens of wound burn infections. Journal of traditional and complementary medicine 2020 Jan12; 10(1), 13-25. doi:10.1016/j.jtcme.2019.01.004

  • Jitan, S. A., AlKhoori, S., Ochsenkühn, M., Amin, S. A., and Yousef, L. F. Ethanol/water extracts from halophyte species Arthrocnemum macrostachyum and Tetraena qatarensis. Cogent Chemistry 2018 Oct 22; 4(1), 1-6. https://doi.org/10.1080/23312009.2018.1536311

  • Balkrishna A, Rohela, A, Kumar A, Kumar A, Arya V, Thakur P, Oleksak P, Krejcar O, Verma R, Kumar D, Kuca K. Mechanistic insight into antimicrobial and antioxidant potential of Jasminum species: A herbal approach for disease management. Plants 2021 May 28;10:1-25. https://doi.org/10.3390/plants10061089

  • Olajuyigbe, O. O., and Afolayan, A. J. Evaluation of combination effects of ethanolic extract of Ziziphus mucronata Willd. subsp. mucronata Willd. and antibiotics against clinically important bacteria. The Scientific World Journal 2013; 1-9. Doi: https://doi.org/10.1155/2013/769594.

  • Saquib, S. A., AlQahtani, N. A., Ahmad, I., Kader, M. A., Al Shahrani, S. S., and Asiri, E. A. Evaluation and comparison of antibacterial efficacy of herbal extracts in combination with antibiotics on periodontal pathobionts: An in vitro microbiological study. Antibiotics 2019 July1; 8(3), 1-12. Doi: 10.3390/antibiotics8030089.


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  • Antioxidant and Antibacterial Potential of Lycium Shawii Roem. And Schult (Awsaj) and Tetraena Qatarensis (Hadidi) Beier and Thulin Leaves: A Herbal Approach to Combat Drug Resistance and Oxidative Stress

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Authors

Mashael W. Alruways
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra, Saudi Arabia
Nazam Khan
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra, Saudi Arabia

Abstract


The present investigation deals with the antioxidant and antibacterial activity of chloroform and methanolic extracts of Lycium shawii and Tetraena qatarensis leaves. Antioxidant activity was evaluated using DPPH and FRAP assay. In the DPPH assay, the lowest IC50 values 45.63 and 46.17μg/ml were observed with methanolic and chloroform extract Lycium shawii leaves, respectively. In FRAP assay most of the extracts showed optical density >0.5 at 40μg/ml. In the antibacterial activity test, all tested extracts were found active towards all bacterial strains with MIC ranged between 0.03125 to 0.12mg/mL. Subsequently, in the combined effect assay, the highest fold increase (92.31%) was observed against Salmonella typhi with methanol extract of Lycium shawii leaves. The examined plants could be used as a treatment for microbial infection and oxidative stress after detailed follow-up investigations.

Keywords


Lycium shawii, Tetraena qatarensis, DPPH, FRAP, Antibacterial activity.

References