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Nam, Seoul-Hee
- Antibacterial Activity of Essential Oils Against Streptococcus Mutans
Authors
1 College of Health Sciences, Kangwon National University, Samcheok-si,25949, KP
2 Dep., of Dental Hygiene, College of Health Sciences, Kangwon National University, Samcheok-si,25949, KP
Source
Medico-Legal Update, Vol 20, No 1 (2020), Pagination: 1804-1809Abstract
Background/Objectives: The purpose of this study was to investigate the antimicrobial activity against bacteria in oral cavity by selecting tea tree and pepper, which are natural essential oils with aroma suitable for oral use
Method/Statistical Analysis: Streptococcus mutans(S. mutans)was used for the antibacterial activity measurement after subculture in brain heart infusion (BHI; Sigma-Aldrich, St. Louis, MO, USA). Tea tree and peppermint essential oil concentrations of 0.5%, 1%, 5%, 10%, 20% and 30% were divided into 5×10 5 colony forming units; CFUs/ml diluted S. mutans10ul, BHI 900ul, 10% Tween 20 90ul was applied to prepare the final 1mL to check the CFUs according to 24h at 37°Ϲ to evaluate the antimicrobial effect.
Findings: The CFUs of the essential oils of tea tree and peppermint showed a reduction of 10 -6 in 30% tea tree compared to the control without natural essential oil, 30% Peppermint showed 10-8 deaths. Although the difference in natural essential oils is different, it is shown that the higher the concentration of the essential oil, the higher the bacterial death.
Improvements/Applications: Peppermint and tea tree essential oils were found to be antimicrobial. Especially, tea tree essential oil has excellent antimicrobial activity compared to peppermint essential oil.
Keywords
Essential Oils, Antibacterial Activity, Streptococcus Mutans, Oral Health, Dental Caries.
- Antimicrobial Activity of Coptischinensis Extract against Dental Caries
Authors
1 Dept. of Dental Hygiene, Hallym Polytechnic University, Chuncheon-si 24210, KP
2 Dept. of Dental Hygiene, College of Health Sciences, Youngsan University, Yangsan-si 51510, KP
3 Dept. of Dental Hygiene, College of Health Sciences, Kangwon National University, Samcheok-si 25949, KP
Source
Medico-Legal Update, Vol 20, No 1 (2020), Pagination: 1907-1912Abstract
Background/Objectives: Many microorganisms reside in the oral cavity, and they are referred to as oral-resident bacteria. Oral microorganisms are an important cause of oral diseases, and as such, many studies have been conducted to control them. This study aimed to investigate the antimicrobial activity against S. intermedius and S. gordoii among the oral-resident bacteria using Coptischinensis, a natural extract.
Method/Statistical Analysis: To investigate the antimicrobial activity of Coptischinensis, the clear-zone confirmation method using filter paper and colony-forming unit (CFU) was used to check the bacterial proliferation. As the concentration of Coptischinensis increased, the size of the clear zone also increased. As a result of the CFU, bacterial proliferation was not observed in the experimental group as compared to the control group.
Findings: The results of this study proved the antimicrobial activity of Coptischinensis against the oral-resident bacteria S. intermedius and S. gordoii. The clear zone results showed that S. intermedius and S. gordoii were larger in size than the control group.CFU showed a large amount of colony in the control group but not exist colony in the experimental group.The result showed its potential as an antimicrobial agent applicable to oral-resident bacteria.
Improvements/Applications: S. intermedius and S. gordoii bacteria found in the oral cavity showed antimicrobial effect on the extracts. Coptischinensis was confirmed to be a preventative and therapeutic agent of tooth decay. In conclusion, the possibility that it can be applied to various intraoral antibacterial products in the future.
Keywords
Antibacterial Activity, Coptischinensis, Dental Caries, Dental Pathogens, Microorganism.- Anticariogeniceffect of Sambucuswilliamsii var. Coreana NAKAI (S. Williamsii) Extract on Against Streptococcus Mutans
Authors
1 Dep, of Dental Hygiene, College of Health Sciences, Kangwon National University, Samcheok-si, 25949, KP
Source
Medico-Legal Update, Vol 20, No 1 (2020), Pagination: 2020-2024Abstract
Background/Objectives: The purpose of the present study was to assess the anticariogenic effects of Sambucuswilliamsii var. coreana NAKAI(S. williamsii) extract against Streptococcus mutans(S. mutans).
Method/Statistical Analysis: S. williamsii purchased from Foodsynergy Co., Ltd. (Seoul, South Korea). Dried S. williamsii were then crushed and percolated in 70% ethanol, and concentrated S. williamsii extract was applied to S. mutansdiluted to 5×10 5 CFU/ml at the concentrations of 0, 2.5, 5, 10, 20, and 30 mg/ml. Then the colony-forming units (CFUs) were evaluated at 6 and 24 h.
Findings: The S. williamsii extract against S. mutanshad an minimal inhibitory concentration (MIC) of 20 mg/ml and an minimum bactericidal concentration (MBC) of 30 mg/ml at 6 h, and an MIC of 10 mg/ml and an MBC of 20 mg/ml at 24 h. As the concentration of S. williamsii extract increased, the S. mutans was more clearly killed over time. The application of S. williamsiiextract to S. mutanshas an anti-dental-caries effect.
Improvements/Applications: S. williamsii extract promotes oral health promotion by inhibiting the growth of S. mtans. It could be used as an effective oral anticariogenic agent.