Medico-Legal Update

SMA AL-Kubaisi ¹, Omar-Althani Shareef Saed ², Jassim Mohamed Suleiman ², Mustafa Salah Hasan ¹, Maher Saber Owain ², Ahmed Sami Jarad ¹

Affiliations
1 Department of Internal and Preventive Medicine, College of Vet. Med.,University of Fallujah, IQ
2 Department of Internal and Preventive Medicine, College of Vet. Med., University of Tikrit, IQ

Abstract

A fifty fecal swabs (25 from Anbar province and 25 from Salahuddin province) were collected from both sexes dogs with different global breeds, different ages. These swabs were cultured onto blood , nutrient and MacConkey agars, then the colonies were purified on nutrient and MacConkey agars. A gram stain and biochemical tests were done including catalase, oxidase and TSI. The results of isolation and identification showed that Salmonella spp. were isolated from 5 out of 14 puppies (35.7%), while E. coli from 9 (64.3%) and Serratia spp. from 1(7.1%), whereas, from adults , salmonella spp. isolated from 9 (25%) dogs and E. coli from 24 (66.7%) out of 36 adult dogs. It is concluded that Salmonella spp. and E. coli are the most prevalent in asymptomatic dogs and puppies in Anbar province and Salahuddin which means that the dogs are a reservoir for these bacteria and it is considered as a bad indication for transmission of this pathogen to human being and other animals.

Keywords

Facultative anaerobic, dog, E. coli, Salmonella, puppies

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Jassim Mohamed Suleiman ¹, Omar-Althani Shareef Saed ¹, SMA AL-Kubaisi ², Mustafa Salah Hasan ², Maher Saber Owain ¹, Mohammed Ali Hussein ², Ahmed Sami Jarad ²

Affiliations
1 Department of Internal and Preventive Medicine, College of Vet. Med., University of Tikrit, IQ
2 Department of Internal and Preventive Medicine, College of Vet. Med., University of Fallujah, IQ

Abstract

This study aimed to detect eae and Stx genes of thirty two E. coli O157:H7 isolates recovered from calves. These isolates were isolated and identify by traditional methods of culturing and latex agglutination test from fecal swabs of four diarrheic calves and twenty eight apparently healthy calves. The eae and Stx genes were detected by a realtime PCR applied on the extracted bacterial DNA. The results showed that 19 isolates had stx1 gene, 10 isolates had stx2 gene and 17 isolates had eae gene. It is concluded that molecular characterization by using real time PCR is a good test for confirming infection with E. coli O157:H7 and detection of their eae and Stx genes

Keywords

Real time, PCR, stx, eae, Calves, E. coli O157:H7

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