Medico-Legal Update

Baydaa Lateef Hameed ¹, Mutaz Sabah Ahmeid ², NihadKhalawe Tektook ³, Hashim Abdulsattar Jabar ⁴

Affiliations
1 Kirkuk health directorate, Kirkuk city, IQ
2 M.B.Ch.B, M.Sc, Ph.D in Clinical Biochemistry, College of Medicine, Tikrit University, IQ
3 Ph.D in immunology, Middle Technical University-College of Medical & Health Technology-Baghdad, IQ
4 B.Sc, M.Sc in Chemistry, Department of Biochemistry, College of Medicine, Tikrit University, IQ

Abstract

Objectives: A prospective study carried out in Kirkuk city from 15th of January 2019 to 10th of April 2019, included 45 women who were enrolled in assisted reproductive technology (ART) programs in infertility Centre/Kirkuk, Iraq. Patients and Method: All women were subjected to the basic fertility work-up at the infertility center which consists of history taking,physical examination, ovulation detection, evaluation of tubal patency and uterine cavity. The average age of the included women ranged between 20 and 42 years old. All women were enrolled in short protocol type of IVF/ICSI cycle and they had normal menstrual cycles. Nearly 5 ml of follicular fluid was obtained from all women undergoing ICSI for estimation of follistatin level by ELISA technique. Results: The present study showed that 31.1% (14 of 45) of women underwent ISCI technique became pregnant and 68.9% were non-pregnant (31 of 45). The highest mean of age were recorded among pregnant women compared with non-pregnant women (32.21 ± 6.68 v.s. 31.80 ± 5.38 year) although the result was non-significant (P: > 0.05). The highest mean of BMI were recorded among non-pregnant women compared with pregnant women (23.92 ± 1.55 v.s 25.36 ± 1.99 kg/m²), the result was significant. The highest mean level of follicular fluid follistatin evaluated in pregnant compared with non pregnant (10.20 ± 0.30 v.s 9.83 ± 0.58 ng/ml) although the result was high significant difference (p: < 0.05). The study showed that there was negative correlation of follicular fluid follistatin level with age in pregnant women (r= -0.3), with positive correlation of with age in non-pregnant women. Conclusions: There was a highly significant relation of follicular fluid follistatin with pregnancy outcomes after ICSI.

Keywords

Follistatin, Follicular Fluid, ICSI, Infertility, Pregnancy.

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Hibah Hasan Ibrahim ¹, Mutaz Sabah Ahmeid ², Nihad Khalawe Tektook ³, Hashim Abdulsattar Jabar ⁴

Affiliations
1 Kirkuk Health Directorate, Kirkuk City, IQ
2 M.B.Ch.B, M.Sc, Ph.D in Clinical Biochemistry, College of Medicine, Tikrit University, IQ
3 Ph.D in Immunology, Middle Technical University, College of Medical & Health Technology, Baghdad, IQ
4 B.Sc, M.Sc in Chemistry, Department of Biochemistry, College of Medicine, Tikrit University, IQ

Abstract

Background: Klotho, is a protein associated with life extension plays a important role in kidney disease progression, anti-aging, anti-oxidation, modulation of ion transport, and development of disturbed mineral metabolism. The aim of the study was to measure Soluble α-Klotho(SAKL) levels in chronic kidney disease(CKD) patients before and after dialysis in relation with Lipid profile. Methods: This shortprospective hospital-based study was done in the Department of Chemistry and Biochemistry, College of medicine, Tikrit University, Tikrit, Iraq. The study was carried out for 30 patients subjected to Hemodialysis recruited from Tikrit Teaching Hospital, hemodialysis unit between 1st December, 2018 and 1st April, 2019. The study also included 30 adult persons looks healthy with no prior medical or family history of CKD as a control participated in this study. The levels of SAKL and lipid profile were measured in the serum of 30 patients, before and after Hemodialysis and compared with controls. Results: The study revealed increased in the SAKL level in CKD patients before dialysis compared to healthy control group, and decreased in group of CKD after dialysis compared to control group. Furthermore, there was a significant positive correlation of SAKL level with serum Triglyceride and Very low density lipoprotein cholesterol(VLDL-C) in CKD patients before dialysis. Conclusions: There was a highly significant relation of SAKL with lipid abnormality in CKD patients under hemodialysis.

Keywords

Soluble α,-Klotho, Chronic Kidney Disease, Hemodialysis, Lipid Profile.

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Ahmed Abduljabbar Hameed ¹, Mutaz Sabah Ahmeid ², Mutaz Sabah Ahmeid ²

Affiliations
1 Obstetrics and Gynecology of Alzahra’a Hospital, Almosyab City, IQ
2 M.B.Ch.B., M.Sc., Ph.D. in Clinical Biochemistry, College of Medicine, Tikrit University, IQ

Abstract

Background: Polycystic Ovarian Syndrome(PCOS) is one of the most frequent gynecological endocrinopathy that occur in premenopausalfemales, with a predominance range from 6%to10% among women of reproductive age. Melatonin (N-acetyl-5-methoxytryptamine), an neurohormone synthesized from the aromatic amino acid tryptophan mainly by the pineal gland of humans.The important of melatonin in human reproduction is still unidentified. A numerous study suggests that melatonin and reproductive hormones are closely related. This concept is based on observation of influence melatonin on sex steroid production at different stages of ovarian follicular maturation as melatonin receptors have been demonstrated at multiple sites in ovary and in intrafollicular fluid. Aim: The study aim to evaluate the role of melatonin in women with polycystic ovarian syndrome. Materials and Methods: A prospective cross-sectional study was designed taking 60 PCOS patients and 30 controls women whose ages were between 15-35 years old, from beginning of January 2019 to ending of April 2019. These patients admitted to obstetrics and gynecology unit at Babylon teaching hospital. And Five ml of blood sample was taken on day 2 of menstrual cycle by vein puncture from each subject enrolled in this study. Blood samples were collected between 07:00 am–8:00 am, and placed into sterile test tubes to serum melatonin estimaResult:tion.  The study showed that there is the significant difference between PCOS women and the control group concerning melatonin level,and the highest mean of melatonin was occurred in PCOS women (61.21 ± 13.78 vs. 28.61 ± 6.73). And also This study showed that there were the higher means of melatonin in PCOS women with positive family history than PCOS women with negative family history (66.56 ± 11.25 vs. 59.26 ± 14.21) with significant relation. Conclusions: Women with PCOS have significant increase of serum melatonin in PCOS women. And also thisstudy could open the way for therapeutic role of melatonin in treatment of PCOS women.

Keywords

Melatonin, Iraqi Patients, Polycystic Ovarian Syndrome.

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