Medico-Legal Update

Saja Mohammed Mohsen ¹, Abbas Abood Farhan ², Mohammed Abdul-Daim Saleh ³

Affiliations
1 Middle Technical University, Baquba Technical Institute, Baquba, IQ
2 Department of Biology - College of education for pure science- Diyala University- Diyala, IQ
3 Department of Biotechnology - College of Science -Diyala University- Diyala, IQ

Abstract

Renal failure (RF) is common disease, it is decrease of kidney function, glomerular filtration rate (GFR) this use to estimate the kidney function reduction usually is less than 60 mL / min, depending on global Burden of Disease, and the disease is ninth cause of death in the world, According to the global Burden of Disease. Our study is investigated in the relationship between IL-17F (rs763780) genes polymorphic variants in renal failure patients in Iraq. This study was accompanied around Iraqi renal failure patients, the period from February to November 2018. This study included 70 patients from Abn-Sina Center for dialysis and kidney disease, and 30 healthy controls. Tetra-primer ARMS-PCR is rapid, effective and developed assay used our study for studying in genotyping polymorphism of IL-17F (rs763780) genes. Result of our report including the correlation between the genotypes of IL-17F (rs763780) and the incidence of renal failure, as the results show a non-marked difference between patients and controls when genotype T/T (OR = 0.75). Also, no significant difference between patients and control when genotype C/C (OR = 0.85), while the genotype T/C (OR = 2.23) show important difference between control and patients more than two times. In conclusion, IL-17F play an essential role as pro-inflammatory cytokine in renal failure patients and development disease.

Keywords

renal failure patients, rs763780 , ARMS-PCR and IL-17F gene

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Asaal Abdul-Ghani Shyeaa ¹, Mohammed Abdul-Daim Saleh ², Nihad Khalawe Tektook ³

Affiliations
1 Department of Biology, College of Science, Diyala University, Diyala, IQ
2 Department of Biotechnology, College of Science, Diyala University, Diyala, IQ
3 College of Health and Medical Technics, Middle Technical University, Baghdad, IQ

Abstract

Objectives: The present study designed to investigate the Immunological role of toll-like receptor-7, IL-17A and IL-10 in sera of 66 Chronic Renal Failure CRF patients at IBN-SINA center for Dialysis in Diyala governorate - Iraq. Methods: Sandwich Enzyme Linked Immuno-Sorbent Assay(ELISA) technique were used in this study to evaluate concentration of TLR-7 ,IL-17A and IL-10 between study groups. Results: The findings of the present study demonstrated that significantly increased level of TLR-7 in the CRF group (0.24± 0.05 ng/ml) compared to controls (0.02± 0.08 ng/ml), the findings revealed a significant association between CRF and IL-17A serum level in the studied groups,There was a significantly increased level of IL- 17A in the CRF group (6.50±1.37 pg/ml) compared to controls (2.19± 0.32 pg/ml) also decreased level of IL-10 in the CRF group (26.20± 5.16 pg/ml) compared to controls (79.27±23.44 pg/ml). Conclusion: These findings concluded that TLR-7, IL-17A and IL-10 might have a role in CRF pathogenesis.

Keywords

Chronic Renal Failure, TLR-7, IL-17A, IL-10, Pro-Inflammatory Cytokines.

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