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John Kirubaharan, J.
- Influence of Storage Methods of Whole Blood Samples on DNA Integrity in Epidemiological Studies
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Method and materials: Whole blood samples were collected from 90 patients reported to the Government Tertiary-care Dental College and Hospital, Tamil Nadu, India. The blood samples were stored at three different temperatures -80 °C, -20 °C, 4 °C which were analyzed for DNA quality immediately within 4 h and after a storage period of 60 days. Quantification of DNA in the sample was recorded using a spectrophotometer..
Results: The average concentration of isolated DNA ranged from 0.5 ng/μl to 154.2 ng/μl with purity A260/A280ratio ranging from 0.7 to 2.0. The mean DNA concentration H = 97.2 (p = 0.001) and purity H = 80.1 (p = 0.001) obtained from whole blood stored at 4 °C were statistically significant. Thus samples stored at 4 °C did not affect on DNA yield and quality..
Conclusion: We conclude that storage at 4 8C temperature for whole blood does not substantially affect the molecular assay measurements. This study enlightens the influence of storage temperatures and duration in genetic epidemiology.
Authors
C. Rahila
1,
M. B. Aswath Narayanan
2,
J. John Kirubaharan
3,
S .G. Ramesh Kumar
2,
A. Leena Selvamary
2,
A. Sujatha
2
Affiliations
1 Department of Public Health Dentistry, Vivekanandha Dental College and Hospital for Women, Tiruchengode, 637205, IN
2 Department of Public Health Dentistry, Tamilnadu Government Dental College and Hospital, Chennai 600003, IN
3 Department of Veterinary Microbiology, Madras Veterinary College, Chennai 600007, IN
1 Department of Public Health Dentistry, Vivekanandha Dental College and Hospital for Women, Tiruchengode, 637205, IN
2 Department of Public Health Dentistry, Tamilnadu Government Dental College and Hospital, Chennai 600003, IN
3 Department of Veterinary Microbiology, Madras Veterinary College, Chennai 600007, IN
Source
Journal of Pierre Fauchard Academy (India Section), Vol 31, No 2 (2017), Pagination: 89-92Abstract
Objectives: To investigate the influence of storage methods of whole blood samples on Deoxyribonucleic acid (DNA) quality.Method and materials: Whole blood samples were collected from 90 patients reported to the Government Tertiary-care Dental College and Hospital, Tamil Nadu, India. The blood samples were stored at three different temperatures -80 °C, -20 °C, 4 °C which were analyzed for DNA quality immediately within 4 h and after a storage period of 60 days. Quantification of DNA in the sample was recorded using a spectrophotometer..
Results: The average concentration of isolated DNA ranged from 0.5 ng/μl to 154.2 ng/μl with purity A260/A280ratio ranging from 0.7 to 2.0. The mean DNA concentration H = 97.2 (p = 0.001) and purity H = 80.1 (p = 0.001) obtained from whole blood stored at 4 °C were statistically significant. Thus samples stored at 4 °C did not affect on DNA yield and quality..
Conclusion: We conclude that storage at 4 8C temperature for whole blood does not substantially affect the molecular assay measurements. This study enlightens the influence of storage temperatures and duration in genetic epidemiology.
Keywords
DNA Isolation, Genetic Epidemiology, Whole Blood.
References
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