Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Comparative Evaluation of Tissue Dissolution Efficacy of Azadirachta indica Irrigant and Sodium Hypochlorite – An in vitro Study


Affiliations
1 Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Vishnupur, Bhimavaram, West Godavari District - 534202, Andhra Pradesh, India
2 Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Vishnupur, Bhimavaram, West Godavari District - 534202, Andhra Pradesh
     

   Subscribe/Renew Journal


Objectives: This study aimed to evaluate the tissue dissolution efficacy of Azadirachta indica (A. indica) irrigant and sodium hypochlorite. Materials and Methods: 40 bovine muscle tissue specimens of equal dimensions were used in the experiment. Based on tissue specimen treatment, they were divided into four test groups - 3%NaOCl (Group I), 7.5% A. indica irrigant (Group II), alternative use of 3%NaOCl and 7.5% A. indica irrigant (Group III) and control group as distilled water (Group IV). Tissue specimens were weighed before and after placing in test solutions at 2 different time intervals (30,60 minutes). Results: The results revealed that 3%NaOCl showed highest tissue dissolution followed by alternative use of 3%NaOCl. and A. indica irrigant, A. indica alone and distilled water (Group I >III>II>IV). The larger the duration of contact with irrigant better is the tissue dissolution. Conclusion: 7.5% A. indica irrigant alone does not have any tissue dissolving property but in combination it acts as a neutral solution neither increasing nor decreasing the capability of 3%NaOCl.

Keywords

Azadirachta indica, NaOCl, Smear Layer, Tissue Dissolution
User
Subscription Login to verify subscription
Notifications
Font Size

  • Stojicic S, Zivkovic S, Qian W, Zhang H, Haapasalo M. Tissue dissolution by sodium hypochlorite: effect of concentration, temperature, agitation, and surfactant. J Endod. 2010;36(9):1558-62. PMid:20728727
  • Ballal NV, Mala K, Bhat KS. Effect of maleic acid and ethylenediaminetetraacetic acid on the dissolution of human pulp tissue – An in vitro study. Int Endod J. 2011;44:353– 356. PMid:21219363
  • Cobankara FK, Ozkan HB, Terlemez A. Comparison of organic tissue dissolution capacities of sodium hypochlorite and chlorine dioxide. J Endod. 2010;36(2):272–274. PMid:20113788
  • Aarati N, Ranganath NN, Soumya BG, Kishore B, Mithun K. Evaluation of antibacterial and anticandidal efficacy of aqueous and alcoholic extract of neem (Azadirachta indica) an invitro study. IJRAP. 2011;2(1):230–235.
  • Chhibber S, Sharma N. Medicinal and therapeutical potential of neem (Azadirachta indica). A Review International Journal of Scientific and Research Publications. 2014;4(5):1–5.
  • Dutta A, Kundabala M. Comparative anti-microbial efficacy of Azadirachta indica irrigant with standard endodontic irrigants: A preliminary study. J Conserv Dent. 2014;17(2):133–137. PMid:24778508. PMCid:PMC4001268
  • Tandan S, Chandra S, Gupta S, Tripathi H, Lal J. Pharmacological effects of Azadirachta indica leaves. Fitoterapia. 1990;LXI:75–78.
  • Irala LE, Grazziotin-Soares R, Salles AA, Munari AZ, Pereira JS. Dissolution of bovine pulp tissue in solutions consisting of varying NaOCl concentrations and combined with EDTA. Braz Oral Res. 2010;24(3):271–276. PMid:20877962
  • Taneja S, Mishra N, Malik S. Comparative evaluation of human pulp tissue dissolution by different concentrations of chlorine dioxide, calcium hypochlorite and sodium hypochlorite: An in vitro study. J Conserv Dent. 2014;17(6):541–545. PMid:25506141. PMCid:PMC4252927
  • Christensen CE, McNeal SF, Eleazer P. Effect of Lowering the pH of sodium hypochlorite on dissolving tissue in Vitro. J Endod 2008;34:449–452. . PMid:18358894
  • Moorer WR, Wesselink PR. Factors promoting the tissue dissolving capability of sodium hypochlorite. Int Endod J. 1982;15:187–196.
  • Rossi-Fedele G, Steier L, Dogramaci EJ, Canullo L, Steier G, de Figueiredo JA. Bovine pulp tissue dissolution ability of HealOzone, Aquatine Alpha Electrolyte and sodium hypochlorite. Aust Endod J. 2013;39:57–61. . PMid:23890260
  • Baker, John R. Principles of Biological Microtechnique. A Study of Fixation and Dyeing. London: Methuen & Co. Ltd; 1958.

Abstract Views: 182

PDF Views: 0




  • Comparative Evaluation of Tissue Dissolution Efficacy of Azadirachta indica Irrigant and Sodium Hypochlorite – An in vitro Study

Abstract Views: 182  |  PDF Views: 0

Authors

Girija S. Sajjan
Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Vishnupur, Bhimavaram, West Godavari District - 534202, Andhra Pradesh, India
Podugu Uday Kumar
Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Vishnupur, Bhimavaram, West Godavari District - 534202, Andhra Pradesh, India
T. Rambabu
Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Vishnupur, Bhimavaram, West Godavari District - 534202, Andhra Pradesh, India
K. Madhu Varma
Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Vishnupur, Bhimavaram, West Godavari District - 534202, Andhra Pradesh, India
R. Kalyan Satish
Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Vishnupur, Bhimavaram, West Godavari District - 534202, Andhra Pradesh
G. Prasanthi
Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Vishnupur, Bhimavaram, West Godavari District - 534202, Andhra Pradesh, India

Abstract


Objectives: This study aimed to evaluate the tissue dissolution efficacy of Azadirachta indica (A. indica) irrigant and sodium hypochlorite. Materials and Methods: 40 bovine muscle tissue specimens of equal dimensions were used in the experiment. Based on tissue specimen treatment, they were divided into four test groups - 3%NaOCl (Group I), 7.5% A. indica irrigant (Group II), alternative use of 3%NaOCl and 7.5% A. indica irrigant (Group III) and control group as distilled water (Group IV). Tissue specimens were weighed before and after placing in test solutions at 2 different time intervals (30,60 minutes). Results: The results revealed that 3%NaOCl showed highest tissue dissolution followed by alternative use of 3%NaOCl. and A. indica irrigant, A. indica alone and distilled water (Group I >III>II>IV). The larger the duration of contact with irrigant better is the tissue dissolution. Conclusion: 7.5% A. indica irrigant alone does not have any tissue dissolving property but in combination it acts as a neutral solution neither increasing nor decreasing the capability of 3%NaOCl.

Keywords


Azadirachta indica, NaOCl, Smear Layer, Tissue Dissolution

References





DOI: https://doi.org/10.18311/jpfa%2F2018%2F22121