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Kothari, Vijay
- Anti-oxidant Activity of M. zapota and C. limon Seeds
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Journal of Natural Remedies, Vol 10, No 2 (2010), Pagination: 175-180Abstract
Seed extracts of Citrus limon and Manilkara zapota were analyzed for their antioxidant activity, free radical scavenging activity, and lipid peroxidation inhibition capacity. Total phenolic and total flavonoid contents were also estimated, antioxidant activity was found to be correlated to the former. Chloroform-methanol extract of M. zapota exhibited the highest total antioxidant capacity among all samples tested. The chloroform-methanol solvent mixture proved most efficient in extracting phenolic constituents.Keywords
Antioxidant, Free Radical, Manilkara zapota, Citrus limon, Total Phenolics, Total FlavonoidsFull Text
- Comparative Study of Various Methods for Extraction of Antioxidant and Antibacterial Compounds from Plant Seeds
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Journal of Natural Remedies, Vol 12, No 2 (2012), Pagination: 162-173Abstract
Extracts from seeds of five different plants were prepared in water, methanol, and ethanol by employing five different methods of extraction viz. Soxhlet method, ultrasonication, extraction by continuous shaking at room temperature, and microwave assisted extraction- with and without intermittent cooling. All these extracts were compared with respect to extraction efficiency, total phenol content, total flavonoid content, antioxidant capacity, and antibacterial activity. Soxhlet method proved best in terms of high extraction efficiency, and extraction of phenolic compounds. Microwave assisted extraction with intermittent cooling (MAE), room temperature extraction by shaking (ERT), and ulrasonication assisted extraction (UAE) proved good at extracting antibacterial compounds from plant seeds. Latter also proved effective at extracting antioxidant compounds. Extraction efficiency was found to have no notable correlation with any of the parameters assayed. Methanol proved most suitable solvent for extraction of flavonoids.Keywords
Extraction, Microwave Assisted Extraction (MAE), Ultrasonication Assisted Extraction (UAE), Seeds, FlavonoidsFull Text
- Tamarindus indica (Cesalpiniaceae), and Syzygium cumini (Myrtaceae) Seed Extracts can Kill Multidrug Resistant Streptococcus mutans in Biofilm
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1 Institute of Science, Nirma University, S-G Highway, Ahmedabad, 382481, IN
1 Institute of Science, Nirma University, S-G Highway, Ahmedabad, 382481, IN
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Journal of Natural Remedies, Vol 13, No 2 (2013), Pagination: 81-94Abstract
Extracts of Emblica officinalis seeds prepared by Microwave Assisted Extraction (MAE) method were evaluated for their antimicrobial property against planktonic form of certain human/plant pathogenic microbes. Additionally, seed extracts of E. officinalis, Tamarindus indica, Manilkara zapota, Phoenix sylvestris, Syzygium cumini, and selected phytocompounds were tested against multi-drug resistant Streptococcus mutans (a major pathogen associated with human dental caries) in its planktonic as well as biofilm form. Ability of these extracts to eradicate and kill S. mutans biofilm was investigated. E. officinalis extracts exerted bactericidal action against S. mutans, Pseudomonas aeruginosa, and Vibrio cholerae. Acetone extract of S. cumini, and curcumin were able to inhibit S. mutans at appreciably low concentrations of 50 μg/mL and 20 μg/mL respectively. T. indica and S. cumini seed extracts were able to kill ≥ 80% cells of S. mutans in biofilm, in the concentration range of 500-1000 μg/mL. These extracts were able to achieve ≥ 95% killing of S. mutans biofilm at concentrations ranging from 600-2000 μg/mL. Ability of the potent extracts to kill S. mutans biofilm did not seem to be much dependent on eradication of the biofilm. Extraction efficiency was found to have a good correlation with antibacterial activity.Keywords
Antibacterial, Biofilm, Drug-resistance, Microwave Assisted Extraction (MAE), Minimum Inhibitory Concentration (MIC), Emblica officinalisFull Text
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- In vitro Antibacterial Activity of Manilkara hexandra (Sapotaceae) Seed Extracts and Violacein against Multidrug Resistant Streptococcus mutans
Abstract Views :304 |
PDF Views:169
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Journal of Natural Remedies, Vol 15, No 1 (2015), Pagination: 1-11Abstract
Extracts of the Pongamia pinnata, Manilkara hexandra, and Pyrus pyrifolia seeds prepared by microwave assisted extraction method, and the violet pigment- violacein extracted from Chromobacterium violaceoum were screened for their antibacterial activity against Streptococcus mutans.M. hexandra extracts were able to inhibit both the test strains of S. mutans used in this study with minimum inhibitory concentration (MIC) ranging from 600-800 µg/mL. These extracts exerted bactericidal action against S. mutans with minimum bactericidal concentration (MBC) of 600-900 µg/mL. Acetone extract of M. hexandra seeds registered highest average total activity of 231.20 mL/g. Extraction efficiency was found to have a moderately good correlation with antibacterial activity. Violacein exerted bactericidal action with MIC and MBC of less than 2 µg/mL against both the strains.Keywords
Bactericidal; Biofilm; Microwave assisted extraction; Total activityFull Text