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Patil, Vaishali
- Extraction, Isolation and Structure Elucidation of a Bioactive Constituent from Chloroform Extract of Moringa concanensis Leaves
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Authors
Affiliations
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara, Gujarat, IN
2 Department of Pharmacognosy, Faculty of Pharmacy, Parul Institute of Pharmacy & Research, Parul University, Vadodara, Gujarat, IN
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara, Gujarat, IN
2 Department of Pharmacognosy, Faculty of Pharmacy, Parul Institute of Pharmacy & Research, Parul University, Vadodara, Gujarat, IN
Source
Journal of Natural Remedies, Vol 22, No 4 (2022), Pagination: 673-682Abstract
Moringa concanensis Nimmo. (Moringaceae) is a wild plant found in India. Traditionally, tribes used this plant as an antioxidant, anticancer, anticonvulsant, antimicrobial, antifertility, anti-inflammatory, antipyretic, and analgesic. In the present study, the leaves of Moringa concanensis were subjected to successive solvent extraction with five different solvents of increasing polarity, i.e., petroleum ether, chloroform, ethyl acetate, methanol, and water, followed by a phytochemical screening of each extract. The phytochemical study indicated the presence of terpenoids, steroids, cardiac glycosides, flavonoids, alkaloids, carbohydrates, proteins, tannins, and phenolics. The chloroform extract showed the presence of cardiac glycosides (cardenolides); therefore, a RP-HPLC method was developed to identify the number of constituents in the extract. The extract was recrystallized to isolate the single component and to gain the desired purity for further spectral analysis. Different spectral data from UV-Visible, Infrared, Mass, and Nuclear Magnetic Resonance spectroscopy were analyzed and the isolated chemical constituent was identified.Keywords
Cardiac Glycosides, Infrared Spectroscopy, Mass Spectroscopy, RP-HPLC, Structure ElucidationFull Text
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- Estimation of Digoxin in Chloroform Extract of Moringa concanensis Leaves Using Newly Developed and Validated RP-HPLC Method
Abstract Views :104 |
PDF Views:75
Authors
Affiliations
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
2 Parul Institute of Pharmacy and Research, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
2 Parul Institute of Pharmacy and Research, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
Source
Journal of Natural Remedies, Vol 23, No 1 (2023), Pagination: 149-155Abstract
Cardiac glycosides are the secondary metabolites that are used in atrial fibrillation and atrial flutter as it binds with the sodium-potassium ATPase pump and increases the force of contraction. There are two types of cardiac glycosides i.e. cardenolides and bufadienolides. Digoxin is a cardenolide type of cardiac glycoside. Digoxin is found in chloroform extract of Moringa concanensis leaves. In the present study, the reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated to determine the content of digoxin in chloroform extract of M. concanensis leaves from three different geographical sources. The chromatographic method was developed at 220 nm wavelength with an Acetonitrile: Water: 1% Orthophosphoric acid (50:50:0.1 %V/V/V) mobile phase. In this method, the digoxin shows linearity in the range of 10 – 50 μg/ml with a regression coefficient of 0.995. The Limit of detection (LOD) and Limit of quantitation (LOQ) was found to be 0.232 μg/ml and 0.703 μg/ml respectively.Keywords
Chloroform Extract, Moringa concanensis, RP-HPLC Method, Soxhlet Extraction.Full Text
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