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Journal of Natural Remedies, Vol 1, No 1 (2001), Pagination: 55-59
Abstract
Objective: To develop an improved HPLC method for estimation of phyllanthin and hypophyllanthin in Phyllanthus amarus. Materials and methods: An Isocratic, reversed phase (RP) HPLC procedure has been adopted using a mixture of pH 2.8 Phosphate buffer and acetonitrile as mobile phase, CN column as stationary phase and UV detector. Results: The developed method shows high resolution (R = 1.9), accuracy and reproducibility. Conclusion: The method developed is relatively better in terms of separation than the previously reported methods.
Keywords
Phyllanthus Amarus, Phyllanthin, Hypophyllanthin, HPLC
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