Journal of Natural Remedies

Muntasir M. Majumder , Ehsanul H. Mazumder , M. Ashraful Alam , Raushanara Akter , Mostafizur Rahman , Satyajit D. Sarker

Abstract

The antioxidant potential of the 70% methanolic extract of Ichnocarpus frutescens was assessed by the 1, 1-diphenyl-2-picrylhydrazyl (DPPH), the hydrogen peroxide, the nitric oxide, the reducing power and the lipid peroxidation inhibition (thiobarbituric acid-reactive substances production) and the red blood cell (RBC) membrane stabilization assays. The extract showed significant antioxidant activities in all assays in a dose dependent manner. The extracts displayed notable activities in reactive oxygen species (ROS) scavenging which could be attributed to the high phenolic content of this extract. Moreover, I. frutescens extract showed strong reducing power and an ability to suppress lipid peroxidation. Suppression of lipid peroxidation and nitric oxide scavenging would be the probable mechanism of the stabilization of the RBC membrane. In the DPPH radical scavenging assay the IC₅₀ value of the extract was found to be 23.75 μg/mL. The extract inhibited the nitric oxide radicals generated from sodium nitroprusside and the IC₅₀ value was found to be 69.69 μg/mL, comparable to that of 86.83 μg/mL for the positive control ascorbic acid.

Keywords

Ichnocarpus frutescens, Apocynaceae, Antioxidant, Lipid Peroxidation, Reactive Oxygen Species

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Abbas Delazar , Bohlool H. Asl , Omolbanin Mohammadi , Fariba H. Afshar , Lutfun Nahar , Masoud Modarresi , Hossein Nazemiyeh , Satyajit D. Sarker

Abstract

Eremostachys laciniata (L.) Bunge (family: Lamiaceae) is one of the fifteen endemic Iranian herbs of the genus Eremostachys, and also grows in other central and western Asian countries. In the Iranian traditional medicine, this plant has been used as a local analgesic and as an anti-inflammatory remedy. In the present study, the methanol (MeOH) extract of the rhizomes of E. laciniata as well as the solid-phase extraction (SPE) fractions have been evaluated for their analgesic property in mice using the hot-plate test. All doses of the SPE 20% aq. MeOH fraction (P < 0.01) and the MeOH extract at the doses of 0.5 mg/kg (P < 0.01) and 1 mg/kg (P<0.05) displayed considerable analgesic effects. The analgesic effect of the SPE 20% aq. MeOH fraction (2.5 mg/kg) was more potent than that of the positive control, morphine (9 mg/kg) at 45 min after injection (P<0.001). However, SPE 40% aq. MeOH fraction did not show any analgesic effects at test doses.

Keywords

Eremostachys Laciniata, Lamiaceae, Analgesic, Morphine, Mice, Hot-plate Test

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