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Hilda, Rivanny
- Anti-Inflammatory Potential of Gandarusa (Gendarussa vulgaris Nees) and Soursoup (Annona muricata L) Extracts in LPS Stimulated-Macrophage Cell (RAW264.7)
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PDF Views:186
Authors
Dian Ratih Laksmitawati
1,
Ajeng Prima Prasanti
1,
Nadia Larasinta
1,
Gloria Agitha Syauta
1,
Rivanny Hilda
1,
Hesty Utami Ramadaniati
1,
Anisa Widyastuti
1,
Nadia Karami
1,
Merry Afni
2,
Dwi Davidson Rihibiha
2,
Hanna Sari W. Kusuma
2,
Wahyu Widowati
3
Affiliations
1 University of Pancasila, Jakarta, ID
2 Biomoleculer and Biomedical Research Center, Aretha Medika Utama, Bandung, ID
3 Medical Research Center, Maranatha Christian University, Bandung, ID
1 University of Pancasila, Jakarta, ID
2 Biomoleculer and Biomedical Research Center, Aretha Medika Utama, Bandung, ID
3 Medical Research Center, Maranatha Christian University, Bandung, ID
Source
Journal of Natural Remedies, Vol 16, No 2 (2016), Pagination: 73-81Abstract
Inflammation is one of the important biological responses to injury. Anti-inflammatory is therefore proposed to treat both acute and chronic inflammation. Chemical compounds of various plants are widely used in treatment of inflammation. Objective: This study aims to evaluate anti-inflammatory potential of G. vulgaris extract (GVE) and A. muricata extract (AME) on LPS-stimulated murine macrophage cell line (RAW264.7). Cell viability assay to evaluate nontoxic concentration in cell line was performed with MTS assay. Parameters to determine anti-inflammatory activity between treatment group and non treated cells, were IL-1β, TNF-α, and IL-6 which was measured with Elisa, and NO level which was measured with nitrate/nitrite colorimetric assay. Both GVE and AME of 50 and 10 μg/mL showed high viability (>90%) and it was not significantly different compared to control, makes it suitable for treatment. GVE and AME of 50 μg/mL resulted low TNF-α level in RAW264.7(313.16pg/mL and 264.69 pg/mL respectively), as well as IL-1β level (903.53 pg/mL and 905.00 pg/mL respectively) and IL-6 (175.88 pg/mL and 219.13 pg/mL respectively). Whereas, GVE and AME of 75 μg/mL showed lower NO level (9.76 μM and 9.79 μM respectively) compared to untreated cells. This research revealed that GVE and AME possess the anti-inflammatory potential indicated by inhibition of inflammatory mediators including TNF-α, IL-1β, IL-6 and NO.Keywords
Annona muricata L, Anti-Inflammtory, Gendarussa vulgaris Nees, RAW264.7 Cell Line.Full Text
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