Journal of Natural Remedies

N. Deepa , N. N. Rajendran

Abstract

Objective: To examine the 50% aqueous-ethanol, benzene and chloroform extracts of whole plant of Acanthospermum hispidum DC for their anti-bacterial and anti-fungal properties against a wide range of microorganisms. Method: The anti-bacterial activity was studied by agar dilution method using Muller Hinton (MH) agar media. The results were compared with standard ciprofloxacin (5 μg / disc). The anti-fungal activity of the extracts was investigated by tube dilution method using Sabouraud Dextrose Agar (SDA) medium and the results compared with standard Clotrimazole (125 μg/ml). Result: All studied extracts showed potential anti-bacterial and anti-fungal properties. The MIC (Minimum Inhibitory Concentration) was found to be 66.66 μg / ml for benzene extract and of varying range (66.66-200 μg / ml) for chloroform and 50% aqueous-ethanol extracts, with different micro-organisms. The anti-fungal property of all the studied extracts were comparable with that of standard clotrimazole. Conclusion: The present study brought to light the scientific data on the anti-infective property of the plant.

Keywords

Acanthospermum hispidum DC, Anti-microbial Activity, Anti-bacterial Activity, Anti-fungal Property

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N. Deepa , N. N. Rajendran , T. Lata , N. S. Jagannathan

Abstract

Objective: To examine the anti-bacterial and anti-fungal activities of ethyl acetate extract and the isolated fraction of the plant Acanthospermum hispidum DC. Method: Anti-bacterial activity was studied by agar dilution method using Muller Hinton(MH) Agar media. And the MIC of the extract was compared with that of standard ciprofloxacin (5µg/ml). Anti-fungal activity of the extract was investigated by tube dilution method using Sabouraud Dextrose Agar (SDA) medium and the results compared with standard clotrimazole(125µg/ml). An attempt was made to isolate the fraction responsible for the anti microbial property of the extract. Result: The ethyl acetate extract showed potential anti-bacterial and anti-fungal properties comparable with standard ciprofloxacin and clotrimazole respectively against the organisms examined. The minimum inhibitory concentration (MIC) of the extract for anti-bacterial activity was 200µg/ml. The isolated fraction was also found to possess anti microbial properties similar to that of the crude extract. The MIC of the fraction was 133.33µg /ml. And the thin layer chromatographic study of the fraction showed it as diterpene. Conclusion: The plant appears to be promising for isolation of active constituent for development of phytomedicine for anti-microbial properties.

Keywords

Acanthospermum hispidum DC, Ethyl Acetate Extract, Anti-microbial Activity, Ciprofloxacin, Clotrimazole.

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