Journal of Natural Remedies

M. S. Rajesh , M. S. Harish , R. J. Sathyaprakash , A. Raghuram Shetty , T. N. Shivananda

Abstract

Costus speciosus is known to possess antidiabetic properties and used in local health traditions in India but the validation of drug is not done. The activity of various fractions of drug is also not reported. In view of its immense potential in andiabetic properties, systematic study was conducted with an objective to evaluate the antihyperglycemic activity of petroleum ether, chloroform, methanolic, and aqueous extracts of C. speciosus rhizomes on over night fasted, Streptozotocin (STZ) induced diabetic rats. Blood glucose level (BGL) monitored at 0, 30, 60, 120 and 240 minutes suggested that all extracts of C. speciosus resulted in reduction of BGL significantly except pet ether extract. Aqueous extract and methanolic extracts reduced initial BGL of 387 to 120 mg/dl and 303 to 161 mg/ dl respectively at the end of 240 minutes. Similar studies conducted with oral glucose tolerance test (OGTT) confirmed the above findings suggesting that aqueous extract and methanolic extracts of C. speciosus were highly effective in bringing down the BGL from 590 to 96 mg/dl and 570 to 128 mg/dl respectively at the end of 240 minutes, which was on par with the glibenclamide. Results from multiple dose studies wherein the drug was administered for 14 days also confirmed the above findings and the serum lipid profiles high density lipoproteins (HDL), low density lipoproteins (LDL) and very low density lipoproteins (VLDL) were found to be optimum in aqueous or methanolic extracts on par with normal healthy rats or standard drug glibenclamide treated rats.

Keywords

Costus Speciosus, Aqueous Extract, Methanolic Extract, Chloroform Extract, Petroleum Ether Extract, Sterptozotocin, Anti-hyperglycemic, Diabetic, Wister Rats

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M. S. Rajesh , J. Rajshekar

Abstract

The objective of the study was to screen the extracts of Mangifera indica seed kernel for its antidiabetic activity so that tons of mango seed kernels going waste during the mango season can be utilized as house hold remedy in treating diabetes so that the dose and cost of the actual treatment can be brought down. Extracts were evaluated for their glucose reducing effect in both normal and diabetic rats at the dose of 200 mg/kg body weight. Diabetes was induced by administering streptozotocin at 65 mg/kg body weight in citrate buffer intraperitonially. Blood samples were collected through tip of tail vein and the fasting blood glucose levels were estimated by using reactive strips of glucose oxidase and peroxidase. Serum lipid profiles were carried out by using ready kits by colorimetric method. The results indicate aqueous and methanolic extract of Mangifera indica seed kernel possesses significant antidiabetic activity.

Keywords

Mangifera indica, Seed Kernel, Streptozotocin, Anti-Hyperglycaemic Activity.

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