Journal of Natural Remedies

M. S. Ganachari , Shiv Kumar , K. G. Bhat

Abstract

Objective: To study the effect of hydroalcoholic extract of Ziziphus jujuba leaves on neutrophil phagocytic function. Methods: The different concentrations (5, 10, 25, 50 and100μg/ml) of Ziziphus jujuba leaves extract was subjected, to study its effect on different in vitro methods of phagocytosis such as neutrophil locomotion and chemotaxis test, in vitro immunostimulant activity by slide method and qualitative nitro blue tetrazolium test using human neutrophils. Results: The Ziziphus jujuba leaves extract has stimulated chemotactic, phagocytic and intracellular killing potency of human neutrophils at the concentration range of 5-50μg/ml. Conclusion: The hydroalcoholic extract of Ziziphus jujuba leaves stimulates cell-mediated immune system by increasing neutrophil phagocytic function.

Keywords

Immunostimulant Activity, Neutrophils, Phagocytosis, Ziziphus jujuba

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M. S. Ganachari , Shiv Kumar

Abstract

Objective: To evaluate the antiulcer activity of Ziziphus jujuba leaves extract (ZJE) at various doses using different experimentally induced gastric ulcer models in rats. Methods: Gastric ulcers were induced in rats by Pylorus ligation, 80% ethanol (1ml/rat) and aspirin (200mg/kg). In pylorus ligation induced ulcer model the parameters studied were gastric volume, free acidity, total acidity and ulcer index. Lesion index and gastric mucus content were determined in ethanol induced ulcer model and in aspirin induced ulcer model the ulcer index was determined. Results: In pylorus ligation model, ZJE pretreatment caused significant reduction in gastric volume, free acidity, total acidity and ulcer index as compared to control group. In ethanol-induced ulcers, ZJE was effective in reducing lesion index and increasing the gastric mucus content. It was also effective in decreasing ulcer index in aspirin-induced ulcers. All the results obtained with ZJE were dose dependent. Conclusions: The results suggest that ZJE possesses significant and dose dependent antiulcer activity. The antiulcer activity of ZJE can be attributed to its cytoprotective and antisecretory action.

Keywords

Ziziphus jujuba, Antisecretory, Cytoprotective, Gastric Ulcer

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Shiv Kumar , M. S. Ganachari , Banappa , V. S. Nagoor

Abstract

Objective: To evaluate the anti-inflammatory activity of the alcoholic extract of Ziziphus jujuba Lam leaves in albino rats. Materials and methods: Ziziphus jujuba leaves were extracted with 90 % ethanol and the extract was screened for anti-inflammatory activity at the doses of 200, 400 and 600 mg/kg using acute carrageenan induced paw oedema in albino rats. Diclofenac sodium (100 mg/kg, p.o.) was used as standard reference drug. Results: The % inhibition of paw edema at 3 h after carrageenan administration produced by Ziziphus jujuba leaves extract at the dose of 200, 400 and 600 mg/kg was 44.5%, 62.2% and 81.8% respectively when compared to control. The paw oedema attenuating effect of Ziziphus jujuba leaves extract at the dose of 600 mg/kg was comparable with that produced by diclofenac sodium (88.6%). Conclusion: The present study indicates that Ziziphus jujuba leaves extract possess significant anti-inflammatory activity against carrageenan-induced rat paw edema.

Keywords

Anti-inflammatory, Carrageenan, Inflammation, Ziziphus jujuba

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M. S. Ganachari , Shiv Kumar , K. R. Alagawadi

Abstract

Objective: To evaluate the effect of Ziziphus jujuba leaves extract in cafeteria diet and atherogenic diet induced obesity. Methods: Obesity was induced in albino rats by feeding cafeteria diet/atherogenic diet daily for 40 days in addition to normal diet ad libitum. Body weight was measured on day 1 and then on alternate days for 40 days. Daily food intake for group of 6 rats was assessed. Serum glucose and lipid levels and internal organs and fat pad weight analysis was carried out on day 41. Results: The Z. jujuba leaves extract treatment caused significant reduction in body weight, daily food intake, serum glucose and lipid levels, internal organs and fat pad weights in cafeteria and atherogenic diet fed rats when compared with control group of rats. Conclusion: The results of the present study conclude that alcoholic extract of Z. jujuba leaves showed anti-obese property by decreasing the body weight, food intake, serum glucose and lipid levels and internal organs and fat pad weights in dietary obese rats. The effect produced was comparable with that produced by standard anti-obese drug, Sibutramine.

Keywords

Anti-obese, Atherogenic Diet, Cafeteria Diet, Obesity, Sibutramine, Ziziphus jujuba

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