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George, M. J.
- An Open Label Randomized Control Trial to Assess the Impact of Ayurveda Lifestyle Guidelines and Polyherbal Compounds in Bacterial Flora W.S.R to E. coli and Shigella in Children with Autism Spectrum Disorder
Authors
1 Department of Kaumarabhritya, Vaidyaratnam P S Varier Ayurveda College, Kottakkal – 676501, Malappuram, Kerala, IN
2 Department of Kaumarabhritya, Santhigiri Ayurveda Medical College, Olassery, Kodumba, Palakkad – 678551, Kerala, IN
3 Surya, Kadampazhipuram, Palakkad – 678633, Kerala, IN
4 Department of Shalyatantra, Vaidyaratnam P S Varier Ayurveda College, Kottakkal – 676501, Malappuram, Kerala, IN
5 S.C.S.V.M.V University, Enathur – 631561, Tamil Nadu, IN
Source
Journal of Natural Remedies, Vol 22, No 1 (2022), Pagination: 65 - 74Abstract
Skyrocketed numbers of autism reports with an incremental surge in its prevalence getting multiplied by four in the past decade is wary of an epidemic afoot. A randomised control trial was conducted to evaluate the effectiveness of Ayurveda polyherbal compounds and lifestyle guidelines in addition to the conventional therapies of speech therapy, occupational therapy and behavioral therapy in test group compared to their controls in the bacterial flora for the species of E. coli and Shigella done by 16 S ribosomal RNA sequencing. A clinical trial was conducted at the Out-patient and In-patient units of Vaidyaratnam P. S. Varrier Ayurveda College wherein a total of 60 ASD diagnosed children were included with a random allocation of 1:1 as test group and control group. Test group received Ayurveda poly-herbal formulations and dietary and lifestyle guidelines for 30 days along with multidisciplinary interventions for 2 months however control group received multidisciplinary therapies along with dietary and lifestyle guidelines alone for 2 months. Significant changes were obtained as results with the species of both E. coli and Shigella which was of primordial dominance before treatment has been reduced after treatment in the test group. After one month of medication in the test group much influential positive changes has occurred whereas such significance is not noted in the control group meaning that the conventional therapies of speech therapy and occupational therapy alone could not make an alteration. Polyherbal compounds of Rajanyadi choornam and Vilwadi gulika along with the dietary lifestyle guidelines and multidisciplinary interventions helps the autistic children to reduce their relative abundance for the species of E. coli and Shigella.
Keywords
Autism, Ayurveda Lifestyle Guidelines, E. coli, Gut Micobiota, Rajanyadi choornam, Vilwadi Gulika, Shigella, 16 S r RNA SequencingReferences
- Available from: https://www.ninds.nih.gov/Disorders/ Patient-Caregiver-Education/Fact-Sheets/Autism-Spectrum- Disorder-Fact-Sheet
- Miranda A, Mira A, Berenguer C, Rosello B, Baixauli I. Parenting stress in mothers of children with autism with- out intellectual disability. Mediation of behavioral problems and coping strategies. Front Psychol. 2019; 10:464. https://doi.org/10.3389/fpsyg.2019.00464. PMid:30906274.
- PMCid:PMC6418028
- Xu M, Xu X, Li J, Li F. Association between gut micro-
- biota and autism spectrum disorder: A systematic review and meta-analysis. Front Psychol. 2019; 10:473. https:// doi.org/10.3389/fpsyt.2019.00473. PMid:31404299. PMCid:PMC6673757
- Chaidez V, Hansen RL, Hertz-Picciotto I. Gastrointestinal problems in children with autism, developmental delays or typical development. J. Autism Dev. Disord. 2014; 44(5):1117–27. https://doi.org/10.1007/s10803-013-1973-x. PMid:24193577. PMCid:PMC3981895
- Dinesh et al. Concept of human gut microbiome in health and disease - An ayurveda perspective. World J. Pharm. Pharm. Sci. 2020; 9(5):771–8.
- Ragupathi NKD, Sethuvel DPM, Inbanathan FY, Veeraraghavan B. Accurate differentiation of Escherichia coli and Shigella serogroups: Challenges and strate- gies. New Microbes New Infect. 2017; 21:58–62. https:// doi.org/10.1016/j.nmni.2017.09.003. PMid:29204286. PMCid:PMC5711669
- Pettengill EA, Pettengill JB, Binet R. Phylogenetic analy- ses of shigella and enteroinvasive escherichia coli for the identification of molecular epidemiological markers: whole-genome comparative analysis does not support dis- tinct genera designation. Front Microbiol. 2016; 6:1573. Erratum in: Front Microbiol. https://doi.org/10.3389/ fmicb.2015.01573. PMid:26834722. PMCid:PMC4718091
- Xu M, Xu X, Li J, Li F. Association between gut micro- biota and autism spectrum disorder: A systematic review and meta-analysis. Front Psychol. 2019; 10:473. https:// doi.org/10.3389/fpsyt.2019.00473. PMid:31404299. PMCid:PMC6673757
- Adams JB, Johansen LJ, Powell LD, Quig D, Rubin RA. Gastrointestinal flora and gastrointestinal status in children with autism--comparisons to typical children and correla- tion with autism severity. BMC Gastroenterol. 2011; 11:22. Published 2011 Mar 16. https://doi.org/10.1186/1471- 230X-11-22. PMid:21410934. PMCid:PMC3072352
- Gagliardi A, Totino V, Cacciotti F, et al. Rebuilding the gut microbiota ecosystem. Int. J. Environ. Res. Public. Health. 2018; 15(8):1679. https://doi.org/10.3390/ijerph15081679. PMid:30087270. PMCid:PMC6121872
- The Ayurvedic Formulary of India, Part I; p. 523–4.
- The Ayurvedic Formulary of India, Part I; p. 343–4.
- Subramanian DK, Patel A, George MJ, Sugunanandagopan
- SC, Krishna S, Kelu SV, et al. Effect of Ayurveda gut therapy protocol in managing dysbiosis of children with autism: Study protocol for a randomized controlled trial. Asia Pac. J. Clin. Trials. Nerv. Syst. Dis. 2020; 5:58–64. https://doi. org/10.4103/2542-3932.304945
- Chan AW, Tetzlaff JM, Altman DG, Laupacis A, Gøtzsche PC, et al. SPIRIT 2013 statement: defining standard pro- tocol items for clinical trials. Ann. Intern. Med. 2013; 158(3):200–7. https://doi.org/10.7326/0003-4819-158-3- 201302050-00583. PMid:23295957. PMCid:PMC5114123
- Martinson JNV, Walk ST. Escherichia coli residency in the gut of healthy human adults. Eco. Sal. Plus. 2020; 9(1):10. https://doi.org/10.1128/ecosalplus.ESP-0003-2020. PMid:32978935. PMCid:PMC7523338
- Schroeder GN, Hilbi H. Molecular pathogenesis of Shigella spp.: Controlling host cell signaling, invasion, and death by type III secretion. Clin. Microbiol. Rev. 2008; 21(1):134–56. https://doi.org/10.1128/CMR.00032-07. PMid:18202440. PMCid:PMC2223840
- Gwimbi P, George M, Ramphalile M. Bacterial contamina- tion of drinking water sources in rural villages of Mohale Basin, Lesotho: exposures through neighbourhood sani- tation and hygiene practices. Environ. Health. Prev. Med. 2019; 24:33. https://doi.org/10.1186/s12199-019-0790-z. PMid:31092211. PMCid:PMC6521341
- Ananth M, Rajesh R, Amjith R, et al. Contamination of household open wells in an urban area of Trivandrum, Kerala State, India: A spatial analysis of health risk using geographic information system. Environ. Health. Insights. 2018; 12. https://doi.org/10.1177/1178630218806892. PMid:30369786 PMCid:PMC6201171