Journal of Natural Remedies

M. B. Shankar , J. R. Parikh , M. Geetha , R. S. Mehta , A. K. Saluja

Abstract

Objective: To study hepatoprotective activity of a new benzopyrone derivative (TP) isolated from the alcoholic extract of aerial parts of Tephrosia purpurea Pers. Materials and methods: Compound TP was isolated from alcoholic extract of T. purpurea by normal phase column chromatography using toluene: ethyl acetate (70:30) as mobile phase and structure was elucidated by spectroscopic methods. Hepatoprotective activity of TP and the alcoholic extract was evaluated using carbon tetrachloride, paracetamol and rifampicin as toxicants. The potency of TP (100 mg/kg) and the alcoholic extract of aerial parts (200 mg/kg), was compared with silymarin (100 mg/kg). The SGOT, SGPT, total and direct bilirubin levels of blood were measured spectrophotometrically. Results: Compound TP was found to be 3-hydroxy, 6-methoxy, 2-oxy (3-butanone), 7 (dioxolane-4-one), 2, 3,-dihydrobenzopyrone. The alcoholic extract and TP caused significant fall in the enzyme levels in serum in rats. Conclusion: Alcoholic extract of T. purpurea and TP have shown significant hepatoprotective activity in rats against all the toxicants that were studied.

Keywords

Tephrosia Purpurea, Hepatoprotective Effect, Carbon Tetrachloride, Paracetamol Rifampicin, Silymarin

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M. B. Shankar , J. R. Parikh , M. Geetha , R. S. Mehta , A. K. Saluja

Abstract

Objective: To study antidiabetic activity of two novel androstane derivatives (JB1 and JB2) isolated from the ethanolic extract of seeds of Syzygium cuminii Linn. Materials and methods: Compounds JB1 and JB2 were isolated from ethanolic extract of seeds of S. cuminii by normal phase column chromatography using toluene: ethyl acetate (80:20) as mobile phase and their structure was elucidated by spectroscopic methods. Antidiabetic activity of JB1 and JB2 and the ethanolic extract was evaluated using alloxan as diabetogenic agent. The potency of JB1 and JB2 (100 mg/kg) and the ethanolic extract (200 mg/kg), was compared with glibenclamide (5 mg/kg). The elevated blood glucose levels were monitored colorimetrically using glucose oxidase enzyme system. Results: Compound JB1 was found to be 3, 15-dihydroxy Δ³ androstene [16, 17-C] (6' methyl, 2'- 1, 3-dihydroxy-1-propene) 4H pyran whereas JB2 to be 3-hydroxy androstane [16, 17-C] (6' methyl, 2'-1-hydroxy-isopropene-1-yl) 4, 5, 6 H pyran. Ethanolic extract of seeds of S. cuminii reduced elevated blood glucose levels in alloxan-induced diabetes in rats on single dose as well as prolonged treatment. Compound JB1 was found to be more effective than JB2 as it showed peak activity at the end of day three, which was comparable to ethanolic extract.

Keywords

Syzygium cuminii Linn., Anti-diabetic Activity, Glibenclamide, Alloxan

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