Journal of Natural Remedies

B. C. Hatapakki , H. M. Suresh , Vijayashree Bhoomannavar , S. I. Shivkumar

Abstract

Objective : To evaluate the antidiabetic activity of flowers of Cassia auriculata Linn against alloxaninduced diabetes in rats and to isolate the bioactive constituents from the activity guided fraction. Materials and methods : The ethanol & aqueous extracts of flowers of Cassia auriculata were obtained by continuous soxhlet extraction & cold maceration respectively. Each extract was assessed for antidiabetic activity by estimating serum glucose level in diabetic rats. The active ethanol extract was subjected to qualitative chemical analysis to identify the active phytoconstituents. The sterol; β sitosterol, was isolated from ethanol extract by column chromatography. Results : In alloxan-induced diabetic rats the ethanol extract (250 mg / kg, p.o.) showed significant (P<0.001) antidiabetic activity as observed from serum glucose level in diabetic rats. However, the aqueous extract (250 mg / kg, p.o.) did not significantly reduce the serum glucose level in diabetic rats. The ethanol extract showed the presence of sterols, triterpenoids, flavonoids and tannins. Conclusion : From the results, it is revealed that, the active ethanol extract of Cassia auriculata flowers is worthwhile to develop the bioactive principle for diabetes mellitus and it is also concluded that the isolated sterol; β sitosterol, could be attributed for antidiabetic activity.

Keywords

Cassia auriculata, Flowers, Antidiabetic, Sterols, Ethanol Extract

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B. C. Hatapakki , V. I. Hukkeri

Abstract

Objective : To evaluate ethanol & aqueous extracts of ischolar_main of Marsdenia tenacissima Wight & Arn. at different doses (100 mg/kg, and 200 mg/kg p.o.) for antipyretic activity in yeast induced pyrexia in rats. Materials and Methods : The ethanol extract of ischolar_main of Marsdenia tenacissima was obtained by continuous soxhlet extraction and aqueous extract by cold maceration process. Both the extracts were subjected to phytochemical investigation to identify the phytoconstituents and further assessed for antipyretic activity by yeast induced pyrexia in experimental rats and compared with standard drug, Paracetamol. Results : Upon evaluation of antipyretic activity on experimental animals, the ethanol and aqueous extracts at the dose (100 mg/kg, and 200 mg/kg p.o.) showed significant ( P < 0.01 ) antipyretic activity as observed from evaluation parameter. The ethanol and aqueous extracts revealed the presence of steroid glycosides, alkaloids, saponins on qualitative chemical tests. Conclusion : From the results, it can be concluded that the active ethanol and aqueous extracts (100 mg/kg and 200 mg/kg p.o.) of ischolar_main of Marsdenia tenacissima is worthwhile to develop the bioactive principles for antipyretic activity.

Keywords

Antipyretic, Marsdenia tenacissima, Root, Ethanol Extract, Aqueous Extract

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H. M. Suresh , B. C. Hatapakki , S. I. Shivakumar , C. S. Hallikeri , B. M. Swamy , V. K. Chandur

Abstract

Objective: To evaluate ethanol extract of pods of Cassia auriculata Linn at different doses (100, 200 mg / kg, p.o.) for laxative activity in rats. Materials and Methods: The ethanol extract of pods of Cassia auriculata was obtained by continuous soxhlet extraction and further assessed for laxative activity by charcoal meal test and feacal output in experimental rats and compared with standard senna. The extract was subjected to qualitative chemical analysis to identify the phytoconstituents. Results: Upon evaluation of Laxative activity on experimental animals, the ethanol extract at the dose 200 mg / kg, p.o. showed significant (P<0.001) laxative activity as observed from different evaluation parameters. However, the same at the dose 100 mg / kg, p.o. was less significant. The ethanol extract showed the presence of flavonoids, triterenoids, tannins, sterols and anthracene derivatives. Conclusion: From the results, it is revealed that, the active ethanol extract (200 mg / kg, p.o.) of Cassia auriculata pods is worthwhile to develop the bioactive principle for laxative activity and it is also concluded that, the anthracene derivatives present, could be attributed for the laxative activity.

Keywords

Cassia auriculata, Pods, Laxative, Anthracene Derivatives, Ethanol Extract

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H. M. Suresh , B. C. Hatapakki , S. I. Shivakumar , C. S. Hallikeri , B. M. Swamy , V. K. Chandur

Abstract

Objective: To evaluate ethanol extract of pods of Cassia auriculata Linn at different doses (100, 200 mg / kg, p.o.) for laxative activity in rats. Materials and Methods: The ethanol extract of pods of Cassia auriculata was obtained by continuous soxhlet extraction and further assessed for laxative activity by charcoal meal test and feacal output in experimental rats and compared with standard senna. The extract was subjected to qualitative chemical analysis to identify the phytoconstituents. Results: Upon evaluation of Laxative activity on experimental animals, the ethanol extract at the dose 200 mg / kg, p.o. showed significant (P<0.001) laxative activity as observed from different evaluation parameters. However, the same at the dose 100 mg / kg, p.o. was less significant. The ethanol extract showed the presence of flavonoids, triterenoids, tannins, sterols and anthracene derivatives. Conclusion: From the results, it is revealed that, the active ethanol extract (200 mg / kg, p.o.) of Cassia auriculata pods is worthwhile to develop the bioactive principle for laxative activity and it is also concluded that, the anthracene derivatives present, could be attributed for the laxative activity.

Keywords

Cassia auriculata, Pods, Laxative, Anthracene Derivatives, Ethanol Extract

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S. I. Shivakumar , H. M. Suresh , C. S. Hallikeri , B. C. Hatapakki , J. S. Handiganur , Kuber Sankh , B. Shivakumar

Abstract

Objective: To evaluate the anticonvulsant effect of Cyperus rotundus Linn rhizomes against maximal electroshock (MES) and pentylenetetrazole (PTZ) induced tonic seizers in albino rats. Materials and methods: Ethanol extract of Cyperus rotundus Linn rhizomes were obtained by continuous hot soxhlet extraction. The extract was assessed for anticonvulsant activity by measuring (sec) the duration of tonic flexion, tonic extensor, clonus, stupor and recovery phase in rats. Results: Phytochemical investigation of ethanol extract revealed the presence of flavonoids, vitamins and carbohydrates. The ethanol extract (100 mg / kg, p.o.) reduced hind limb extension and duration of convulsion significantly, (P < 0.001) which was comparable to standard drug phenytoin (25 mg / kg, i.p.) and diazepam (4 mg /kg, i.p.) respectively. Conclusion: The ethanol extract of Cyperus rotundus rhizomes is worthwhile to develop the potent phytoconstituent for treatment of epilepsy and the flavonoids present in ethanol extract could be attributed for anticonvulsant activity.

Keywords

Anticonvulsant, Cyperus Rotundus, Rhizomes, Maximal Electroshock, Pentylenetetrazole

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