Journal of Natural Remedies

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Four successive extracts (petroleum ether, chloroform, methanol and water) of P. granatum fruit rind were tested for in-vitro trypsin inhibitory activity using commercially available enzyme (Porcine pancreatic type II trypsin) and substrate (Benzoyl-D,L-arginine-4-nitroanilide) and the activity was compared with standard trypsin inhibitor from hen egg white (ovomucoid) . The methanolic extract was found to be most active and the activity was comparable to that of the reference standard.

Keywords

Punica Granatum, Ovomucoid, Trypsin Inhibitor, Proteases
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