Journal of Natural Remedies

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Phytochemical Analysis by HR-LCMS and In vitro Anti-diabetic Potential of Michelia champaca Bark


Affiliations
1 Department of Pharmacognosy, School of Pharmacy, GNITC, Hyderabad – 501506, Telangana Department of Pharmacognosy, VELS Institute of Science, Technology and Advanced studies, Chennai – 600117, Tamil Nadu, India
2 Department of Pharmacognosy, VELS Institute of Science, Technology and Advanced studies, Chennai – 600117, Tamil Nadu, India
 

The current analysis was aimed to study the phytochemical profile and in vitro antidiabetic capacity of HEMC bark. HR-LCMS1 was used to identify the phytochemicals present in the extract. The outcomes of HR-LCMS showed the presence of 15 phytochemical compounds. DL-Carnitine, Catechin, D-α-Tocopherol, Colchicine, Myricetin, Epicatechin, Quercetin, Epigallocatechin gallate, Quercetin-3β-D-glucoside, Kaempferol, Sorbic acid, Apocynin, Epigallocatechin gallate, myricetin 3-O-beta-D-galactopyranoside, Naringeninchalcone are the main compounds identified. The inhibition of enzymes like α-amylase and α-glucosidase delays the rate of glucose absorption thus reducing blood glucose levels in the experimental models. The IC50 values of α-amylase and α-glucosidase inhibitory activity of HEMC were acquired to be 88.65 μg/mL and 71.28 μg/mL correspondingly. Positive control acarbose displayed IC50 assessment of 52.94 μg/mL and 50.01 μg/mL correspondingly. Consequently, the current study confirms that HEMC had remarkable antidiabetic activity and hence holds future potential as nutraceuticals in the treatment of diabetes and related ailments.

 


Keywords

HR – LCMS, Antidiabetic, Michelia champaca, Bark, α-amylase, α-glucosidase.
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  • Phytochemical Analysis by HR-LCMS and In vitro Anti-diabetic Potential of Michelia champaca Bark

Abstract Views: 165  |  PDF Views: 78

Authors

Segu Prathyusha
Department of Pharmacognosy, School of Pharmacy, GNITC, Hyderabad – 501506, Telangana Department of Pharmacognosy, VELS Institute of Science, Technology and Advanced studies, Chennai – 600117, Tamil Nadu, India
Malarkodi Velraj
Department of Pharmacognosy, VELS Institute of Science, Technology and Advanced studies, Chennai – 600117, Tamil Nadu, India

Abstract


The current analysis was aimed to study the phytochemical profile and in vitro antidiabetic capacity of HEMC bark. HR-LCMS1 was used to identify the phytochemicals present in the extract. The outcomes of HR-LCMS showed the presence of 15 phytochemical compounds. DL-Carnitine, Catechin, D-α-Tocopherol, Colchicine, Myricetin, Epicatechin, Quercetin, Epigallocatechin gallate, Quercetin-3β-D-glucoside, Kaempferol, Sorbic acid, Apocynin, Epigallocatechin gallate, myricetin 3-O-beta-D-galactopyranoside, Naringeninchalcone are the main compounds identified. The inhibition of enzymes like α-amylase and α-glucosidase delays the rate of glucose absorption thus reducing blood glucose levels in the experimental models. The IC50 values of α-amylase and α-glucosidase inhibitory activity of HEMC were acquired to be 88.65 μg/mL and 71.28 μg/mL correspondingly. Positive control acarbose displayed IC50 assessment of 52.94 μg/mL and 50.01 μg/mL correspondingly. Consequently, the current study confirms that HEMC had remarkable antidiabetic activity and hence holds future potential as nutraceuticals in the treatment of diabetes and related ailments.

 


Keywords


HR – LCMS, Antidiabetic, Michelia champaca, Bark, α-amylase, α-glucosidase.

References





DOI: https://doi.org/10.18311/jnr%2F2022%2F29798