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Total 20 plants of North Maharashtra region were analysed for the presence of protease inhibitors in their seed. Protease inhibitors were detected from proteinaceous seed extracts by employing the rapid X-ray film method described in this investigation. Among studied plants, eleven plant seeds were reported to contain the protease inhibitor capable to inhibit various proteases like trypsin, pepsin, papain and metallo-protease. The testified results were authenticated by quantitative protease inhibition assay. The Albizia amara, Albizia lebbeck, Cassia australis, Mimosa hamata, Peltophorum pterocarpum, reported first time for the presence of trypsin inhibitor.


Protease Inhibitor, Trypsin, Pepsin, Papain, Metallo-Protease, X-Ray Film.
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