Ixora coccinea down Regulate the Inflammation-Associated Prostaglandins (PGE-2), Nitric Oxide (NO) and Cytokines Production in LPS-Stimulated RAW 264.7 Macrophage

Ixora coccinea L. (Rubiaceae) is a traditional herbal medicine for various ailments like cutaneous wounds, infection, hypertension, menstrual irregularities, sprain, chronic ulcer and other skin diseases. The present study investigated the anti-inflammatory effects of I. coccinea leaves methanol extract (IxME) on RAW 246.7 macrophage and its effect on Prostagladins E2 (PGE2), Nitric Oxide (NO) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) production. The experimental findings revealed that IxME treatment inhibited the Cyclooxygenase 2 (COX-2) mediated PGE2 and Nitric Oxide Synthsae (NOS) catalyzed NO production in LPS-treated RAW 246.7 macrophages in a dose dependent manner. On the other hand, production of pro-inflammatory cytokines including TNF-α, IL-6 and IL-1β was significantly decreased as compared to negative control LPS-treated RAW 246.7 macrophages. The findings data coincides and correlated the in vitro anti-inflammatory activity of I. coccinea with previous reports of in vivo anti-inflammatory property.