Refine your search
Collections
Co-Authors
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Sivasubramanian, C.
- Isolation, Identification and Computational Studies of Pseudomonas Putida Evhs2 in Tannery Effluent
Abstract Views :433 |
PDF Views:0
Authors
Affiliations
1 Department of Environment and Herbal Science, Tamil University, Thanjavur – 613 403, Tamil Nadu, IN
2 Department of Biotechnology, Marudupandiar College, Thanjavur – 613 403, Tamil Nadu, IN
1 Department of Environment and Herbal Science, Tamil University, Thanjavur – 613 403, Tamil Nadu, IN
2 Department of Biotechnology, Marudupandiar College, Thanjavur – 613 403, Tamil Nadu, IN
Source
Journal of Computational Intelligence in Bioinformatics, Vol 6, No 1 (2013), Pagination: 69-74Abstract
A study about isolation, identification and analysis of bacteria in tannery effluent. Here the tannery effluent is used as a sample for the entire analysis. A bacterial strain, designated EVHS2 was isolated from the Tannery effluent sample collected from Sembattu, Trichirappalli district, Tamil Nadu, India. It is identified by a series of biochemical tests and the identification is confirmed by using molecular approach. On the basis of the bacterial 16S rRNA sequence, phylogeny and comparison of this gene sequence with the sequence in GENBANK database, it is considered that the isolate is closely related to the members of Pseudomonas putida. Phylogeny and molecular evolutionary analysis were conducted using BLAST (Basic Local Alignment Search Tool) tool. The sequence of 16S rRNA (Pseudomonas putida EVHS2) is submitted to GENBANK in NCBI database (accession number is JF719292.1. It showed 99% of sequence similarity. Separate chromatogram was constructed for both the primers (forward and backward) amplified, using FINCH TV software.Keywords
Pseudomonas Putida EVHS2, Phylogeny, Effluent, FINCH TV, 16S rRNAReferences
- Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman., (1990). “Basic local alignment search tool”. J. Mol. Biol., 215:403–410.
- Amann,R.I., Ludwig, W., and Schleife, K.H.(1995). “Phylogenetic identification and insitu detection of individual microbial cells without cultivation”. Microbiol., 59:143-169.
- Bergey’s Manual., (1984). “Bergey’s Manual of Systematic Bacteriology”. Williams & Wilkins, Baltimore: USA.
- Hugenholtz,P., and Pace, N.R.(1996). “A molecular view of microbial diversity and the biosphere”. Trends Biotechnol., 14,:190.
- Johnson, J.L. and Palleroni, NJ. (1989). “Deoxyribonucleic acid similarities among Pseudomonas species”. International Journal of Systematic Bacteriology, 39: 230235.
- Kersters, K; Ludwig, W; VanCanneyt, M; De Vos, P; Gillis, M and Schleifer, K-H.(1996). “Recent changes in the classification of the Pseudomonas sp: an overview”. Systematic Applied Microbiology, 19: 465−477.
- Palleroni, N.J. (1993). “Pseudomonas classification: A new case history in the taxonomy of Gram-negative bacteria”. Antonie van Leeuwenhoek, 6231251.
- Samanta, S. K., Bhushan, B. and Jain, R. K., (2001). “Efficiency of naphthalene and salicylate degradation by a recombinant Pseudomonas putida mutant strain defective in glucose metabolism”. Appl.Microbiol. Biotechnol.,55, 627–631.
- Woese, C.R, ; Kandler, O. and Wheelis, M.L. (1990). “Towards a natural system of organisms: Proposal for the domains Archea, bacteri and Eucarya”. Proceeding Natural Academy of Science, 7, 4578−4579.
- Woese, C.R. (1987). “Bacterial evolution”. Microbial Review, 6, 60–69.
- Woese, C.R; Blanz, P. and Hahn, C.M., (1984). “What isn't Pseudomonas sp: the importance of nomenclature in bacterial classification”. Systematic Applied Microbiology.5, 179−195.