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Hari, Prashanth P.
- Identification of a Virulent Isolate of the Entomopathogenic Fungus Beauveria bassiana (Balsamo) Vuillemin, its Mass Multiplication and Formulation for Development into a Mycoinsecticide for Management of Helicoverpa armigera (Hubner)
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PDF Views:164
Authors
Affiliations
1 Oilseeds Research, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, IN
1 Oilseeds Research, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, IN
Source
Journal of Biological Control, Vol 23, No 2 (2009), Pagination: 137-144Abstract
Two promising isolates of Beauveria bassiana - ITCC 4513 and HaBb DOR were multiplied on wheat bran supplemented with molasses and yeast extract in a polythene bag with sponge plugs for aeration. The yield of dry conidial powder per polythene bag was 18.25 ± 1.79 g and 10.03 ± 2.47 g for ITCC 4513 and HaBb DOR isolates, respectively. Laboratory bioassays with these conidial powders against 6 days old Helicoverpa armigera larvae resulted in LC50 values of 1.8 x 108 and 2.41 x 108 conidia ml-1 for the ITCC 4513 and HaBb DOR isolates, respectively, 5 days after treatment. Conidia of both the isolates were formulated as Suspension Concentrates (SC) in groundnut, sunflower and mineral oils. The formulations gave a clear suspension in water and could be sprayed with high volume knapsack sprayers. Performance of both isolates when formulated in oils was superior to unformulated conidia as reflected by the higher mortality of H. armigera larvae in laboratory bioassays and field trials on sunflower. The ITCC 4513 isolate was, however, superior to the HaBb DOR isolate in terms of conidial yield, efficacy as an SC formulation against H. armigera and safety to the egg parasitoid Trichogramma chilonis, thus proving to be an ideal candidate for development into a mycoinsecticide.Keywords
Beauveria bassiana, Helicoverpa armigera, Mass Multiplication, Oil Formulation, Suspension Concentrate and Trichogramma.Full Text
References
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- Beauveria bassiana Suspension Concentrate - A Mycoinsecticide for the Management of Helicoverpa armigera (Hubner)
Abstract Views :460 |
PDF Views:226
Authors
Affiliations
1 Directorate of Oilseeds Research, Rajendranagar, Hyderabad 500030, Andhra Pradesh, IN
1 Directorate of Oilseeds Research, Rajendranagar, Hyderabad 500030, Andhra Pradesh, IN
Source
Journal of Biological Control, Vol 23, No 4 (2009), Pagination: 403-408Abstract
A local isolate of the entomopathogenic fungus, Beauveria bassiana (ITCC-4513) was formulated as a Suspension Concentrate (SC) using mineral oil as carrier. In laboratory bioassays against 5-day-old Helicoverpa armigera larvae, the formulation had an LC50 value of 61.22 mg l-1 at 3 days after treatment. Data on shelf-life of the formulation stored in HDPE bottles at room temperature (29±1°C) was generated for 24 months. The formulation was found to be effective at 200 mg l-1 in field trials against H. armigera on sunflower crop. There was no phytotoxic effect of the formulation.Keywords
Beauveria bassiana, Helicoverpa armigera, Suspension Concentrate, Shelf-Life.Full Text
References
- Bateman, R. P., Carey, M., Moore, D. and Prior, C. 1993. The enhanced infectivity of Metarhizium flavoviride in oil formulations to desert locusts at low humidities. Annals of Applied Biology, 122: 145–152.
- Gedela, S. P. 2008. Factors responsible for agrarian crisis in Andhra Pradesh (A Logistic Regression analysis). World Applied Sciences Journal, 4: 707–713.
- Karuppuchamy, P., Balasubramanian, G. and Sundarababu, P. C. 1993. Economic injury level of gram pod borer (Helicoverpa armigera) in sunflower (Helianthus annuus). Indian Journal of Agricultural Sciences, 63: 679–680.
- Moore, D. and Prior, C. 1993. The potential of mycoinsecticides. Biocontrol News and Information, 14: 31N–40N.
- Panchabhavi, K. S. and Krishnamoorthy, P. N. 1978. Estimation of avoidable loss by insect pests on sunflower at Bangalore. Indian Journal of Agricultural Sciences, 48: 264–265.
- Prior, C., Jollands, P. and Le Patourel, G. 1988. Infectivity of water and oil formulations of Beauveria bassiana (Deuteromycotina: Hyphomycetes) to the cocoa weevil Pantorhytes plutus (Coleoptera: Curculionidae). Journal of Invertebrate Pathology, 52: 66–72.
- Vimala Devi, P. S. and Hari, P.P. 2009. Identification of a virulent isolate of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuill.,its mass multiplication and formulation for development into a mycoinsecticide for management of Helicoverpa armigera (Hubner). Journal of Biological Control, 23: 137–144.