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Wadkar, Pallavi N.
- Evaluation and Selection of Efficient Isolates of Trichoderma Species from Diverse Locations in India for Biological Control of Anthracnose Disease of Grapes
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Authors
Affiliations
1 National Research Centre for Grapes, Manjri Farm Post, Pune-Solapur Road, Pune 412 307, Maharashtra, IN
1 National Research Centre for Grapes, Manjri Farm Post, Pune-Solapur Road, Pune 412 307, Maharashtra, IN
Source
Journal of Biological Control, Vol 26, No 2 (2012), Pagination: 50-60Abstract
Thirty four isolates belonging to seven Trichoderma species from different geographical locations of India were screened in vitro for their antagonism to Colletotri gloeosporioides and the efficient isolates were further evaluated for their bioefficacy in vivo and in field for control of anthracnose of grapes. In in vitro screening, all Trichoderma isolates overgrew C. gloeosporioides colony. Microscopic examination of hyphal interaction showed plasmolysis of hyphae of C .gloeosporioides by all Trichoderma isolates. The percent inhibition of radial growth of C. gloeosporioides by toxic volatile and non-volatile metabolites produced by different isolates of Trichoderma ranged from 40.8 to 63.6 and from nil to 65.9 respectively. In in vivo trial with 20 most antagonistic isolates, the lowest disease incidence was recorded in leaves treated with T. harzianum 5R obtained from Kodagu, followed by T. hamatum (NAIMCC- 1717) from Andaman and Nicobar islands and T. asperellum (NAIMCC 1769) from Pitchavaram. Survival studies on grape phylloplane with selected 11 isolates indicated that when applied at 5×106 spores per ml the population ranged from 4087.05 to 1185.42 cfu per cm2 on day 5 which further declined by day 15. On foliar application of these eleven isolates in field, lowest PDI was recorded in T. viride (NAIMCC-1817) initially obtained from soil of western Ghats of Kerala. The other isolates which recorded low PDI values were T. harzianum (NAIMCC-1965) obtained from Darjeeling, T. koningii (NAIMCC-1938), T. pseudokoningii (NAIMCC-1775) obtained from Andaman&Nicobar islands and T. harzianum 5R obtained from Kodagu (Karnataka).Keywords
Colletotrichum gloeosporioides, Trichoderma Spp. Volatile Metabolites, Non-Volatile Metabolites, Lysis and Phylloplane.Full Text
References
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- Bioefficacy of Trichoderma in Reducing Dead Wood Formation and Enhancing Bud Sprouting in Grapevines
Abstract Views :252 |
PDF Views:135
Authors
Affiliations
1 National Research Centre for Grapes, Manjri Farm Post, Pune-Solapur Road, Pune 412 307, Maharashtra, IN
1 National Research Centre for Grapes, Manjri Farm Post, Pune-Solapur Road, Pune 412 307, Maharashtra, IN
Source
Journal of Biological Control, Vol 26, No 3 (2012), Pagination: 260–265Abstract
Pasting of the pruning wounds of grapevines with Tricho-XP, a commercial formulation containing Trichoderma viride and Paecilomyces lecanii, immediately after foundation pruning along with soil application @ 5 g/vine mixed with 245g cattle manure, and spray on the vines @ 1.0 % has shown the potential to reduce dead wood and enhance bud sprouting in Tas-A-Ganesh grapes. Further, five isolates of Trichoderma exhibited differences in their antagonism as well as ability to minimize dead wood formation and enhance bud sprouting. T. harzianum 5R exhibited maximum in vitro antagonism of Lasiodiplodia theobromae and was the most effective in minimizing dead wood formation in grapevines. This isolate was able to grow and inhibit radial growth of L. theobromae even at higher temperature of 36°C.Keywords
Pruning Wound, Lasiodiplodia theobromae, Botryodiplodia theobromae, Alternaria alternata, Trichoderma harzianum 5R.Full Text
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