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Bhat, N. S.
- Molecular Characterization of Bacillus isolates with Insecticidal Activity against Greater Wax Moth, Galleria mellonella L. (Pyralidae: Lepidoptera)
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Authors
Affiliations
1 Department of Entomology, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra (GKVK), Bangalore 560 065, Karnataka, IN
2 Department of Apiculture, University of Agricultural Sciences, GKVK, Bangalore 560 06, IN
1 Department of Entomology, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra (GKVK), Bangalore 560 065, Karnataka, IN
2 Department of Apiculture, University of Agricultural Sciences, GKVK, Bangalore 560 06, IN
Source
Journal of Biological Control, Vol 27, No 4 (2013), Pagination: 298–303Abstract
Six local Bacillus strains isolated from soils from different parts of Karnataka were characterized by their high toxicity against third instar larvae of greater wax moth (Galleria mellonella L.). The PCR amplification analysis using B. thuringiensis specific primers revealed that only four isolates are Bt isolates. The PCR amplification analysis for cry genes indicated the presence of amplified fragments characteristic of three different cry genes cry1Aa, cry2Aa and cry11Aa. Results showed the presence of cry11Aa and cry1Aa in isolate N12 with expected sizes of 816bp and 891-1089bp, respectively. Bt isolate; A-7 harbored cry1Aa, cry2Aa and cry11Aa with product size 891-1308, 235-696 and 636bp, respectively. While the isolates M-2 and A3 contained only one gene cry2Aa with a product size 696-735bp. The two isolates; N-12 and A-7 were harbored multiple cry genes. The cry2Aa genes were the most frequently found among the three local Bt isolates. The profiles of cry genes indicated that all isolates were active against lepidopteran insects, and therefore these strains could be potential candidates in the search for biocontrol agents in management of greater wax moth, G. mellonella with specific lepidopteran activity.Keywords
Galleria mellonella, Bacillus thuringiensis, PCR, cry genes, Lepidoptera.Full Text
References
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- A Biological Approach for Management of Greater Wax Moth, Galleria mellonella L. Using Bacillus thuringiensis
Abstract Views :245 |
PDF Views:132
Authors
Affiliations
1 Department of Entomology, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra (GKVK), Bangalore 560 065, Karnataka, IN
2 Department of Apiculture, University of Agricultural Sciences, GKVK, Bangalore 560 065, IN
3 Bureau of Indian Standards, Parwanoo, Solan 173 220, IN
1 Department of Entomology, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra (GKVK), Bangalore 560 065, Karnataka, IN
2 Department of Apiculture, University of Agricultural Sciences, GKVK, Bangalore 560 065, IN
3 Bureau of Indian Standards, Parwanoo, Solan 173 220, IN
Source
Journal of Biological Control, Vol 27, No 4 (2013), Pagination: 329–333Abstract
Bee keeping has taken a shape of promising enterprise and also becoming popular as one of the components in mixed farming systems. Galleria mellonella L. damage is the major biological constraint in the beekeeping industry. Microbial agent Bacillus thuringiensis and its products have been tried widely against G. mellonella. Our previous study has detected six potential bacterial isolates viz., C7, A3, A7, N12, F2 and M2 active against G. mellonella. The effectiveness of these isolates on the stored honey combs against the G. mellonella was evaluated and the safety of these potential bacterial isolates was tested against honey bees and silk worms. The protection range provided by the isolates varied from 89.5-44.3 per cent and the isolate M2 (89.52%) and standard HD-1 (88.89%) rendered very good protection to the combs from wax moth damage. All the six isolates tested were found safe to honey bee adults and also to larvae indicating that the isolates can be safely deployed under field conditions. Two of the six isolates tested viz., M2 and N12 were toxic to silk worms. The study demonstrated the potentiality of Bt isolates against G. mellonella and their safety to the honey bees and silkworms.Keywords
Galleria Mellonella, Bacillus thuringiensis, Silk Worms, Honey Bees.Full Text
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