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Begum, Mahima
- Eco-Friendly Management of Soil Borne Diseases in Brinjal Through Application of Antagonistic Microbial Population
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Authors
Affiliations
1 Krishi Vigyan Kendra, Sonitpur, Assam, IN
2 Department of Plant Pathology, Assam Agricultural University, Jorhat 785 013, IN
1 Krishi Vigyan Kendra, Sonitpur, Assam, IN
2 Department of Plant Pathology, Assam Agricultural University, Jorhat 785 013, IN
Source
Journal of Biological Control, Vol 27, No 1 (2013), Pagination: 29-34Abstract
In Assam, bacterial wilt, a soil borne disease caused by Ralstonia solanacearum Yabuchi et al. is the major constraint for production of solanaceous vegetables. Present study was made to evaluate the efficacy of substrate based bioformulation of a PGPR, Pseudomonas fluorescens against the bacterial pathogen in brinjal (Solananum melengona) under field condition. Inhibitory activity of P. fluorescens was tested against R. solanacearum following dual culture method. Three substrates, viz., vermicompost (VC), mustard oil cake (MOC) and farm yard manure (FYM) were compared for mass multiplication of the antagonist. The highest population was recorded (105.56 x 108 cfu/g) when mass cultured in VC along with a standard sticker CMC and an osmoticant mannitol. Quantitative assay of population of P. fluorescens revealed that it could maintain high population count up to 180 days of storage at room temperature. Different method of application of the substrate based bioformulations viz., seed treatment (ST), ischolar_main application (RA), soil application at transplanting (SA) and soil application at 30 days after transplanting (30 DAP) and combined application of all the methods showed minimum wilt incidence and maximum disease reduction in brinjal. Minimum wilt incidence (0.25%) was recorded in the treatment comprising combination of ST, RA, SA and 30 DAP with maximum disease reduction. Following the trend of reduction in disease incidence, yield was maximum (34.40 t/ha) in this treatment and also showed the highest recovery of P. fluorescens strain-Pf-D1 in the soil rhizosphere after harvest (77.40 x 108 cfu/g). Vermicompost appeared to be the best nutrient source to support the antagonist for maximum multiplication and disease reduction and combined application of ST, RA, SA, 30 DAP was most effective bacterial wilt disease management in brinjal.Keywords
Antagonist, Bacterial Wilt, Brinjal, Microorganism, Organic Substrate, Pseudomonas fluorescens.References
- Alexander M. 1997. Introduction to soil microbiology. John Wiley and Sons, New York.
- Anonymous. 1999. Indian Horticulture Database. National Horticulture Board, pp. 115–116.
- Anuratha CS, Gnanamanickam SS. 1990. Biological control of bacterial wilt caused by Pseudomonas solanacearum in India with antagonistic bacteria. Pl Soil 124: 109–116.
- Bora LC, Das Minku, Das BC. 2000. Influence of microbial antagonists and soil amendments on bacterial wilt severity and yield of tomato. (Lycopersicon esculentum) Indian J Agric Sci. 70(6): 390–392.
- Bull CT. 1987. Wheat ischolar_main colonization by disease suppressive bacteria and the effect of population size on severity of take-all caused by Gaeumannomyces graminis var. tritici M.S. Fleming West State University, Pullman. pp. 75–81.
- Burr TJ, Schroth MN, Suslow T. 1978. Increased potato yields by treatment of potato seed pieces with specific strains of Pseudomonas fluorescens and P. putida. Phytopathol 68: 1377–1388.
- Dupler M, Baker R. 1984. Survival of Pseudomonas putida, a biological control agent in soil. Phytopathol. 74: 195–200.
- Gohain R. 2001. Application of microbial antagonists for management of bacterial wilt of brinjal. M.Sc. (Agri.) Thesis, Assam Agric. Univ., Jorhat, Assam, India.
- Harman GE, Jin X, Stasz TE, Peruzzotti G, Leapold AC, Taylor AG. 1991. Production of conidial biomass of Trichoderma harzianum for biological control. Biol Cont. 1: 23–28.
- Heijnen CE, Hok-A-Hin CH, Van Veen JA. 1992. Dynamics of Rhizobium leguminosarum biovar. trifolii introduced into soil; the effect of bentonite clay on predation of protozoa. Soil Biol Biochem. 20: 483–488.
- Howie WJ, Cook RJ, Weller DM. 1987. Effects of soil matric potential and cell motility on wheat ischolar_main colonization by fluorescent pseudomonads suppressive to take-all. Phytopathol. 77: 286–292.
- Kohli RR, Srivastava AK, Shivankar VJ. 1988. Organic culture in citrus cultivation. Indian Horti. 43(1): 12–14.
- Loper JE, Stockwell VO. 2000. Current status of biological control of plant diseases. In: Emerging technologies for integrated pest management; concepts, research and implementation. Proc. of a Conference, American Phytopathological Society, USA, 8–10 March, 1999. 240–256.
- Mavrodi OY, Mavrodi DV, Weller DM. 2003. Genes involved in the unique ischolar_main colonizing activity of Pseudomonas fluorescens Q8r1-96. Phytopathol. 93.
- Mulya K, Watanabe M, Goto M, Takikawa Y, Tsuyumu S. 1996. Suppression of bacterial wilt disease of tomato by ischolar_main-dipping with Pseudomonas fluorescens Pf G32 – the role of antibiotic substances and siderophore production. Ann Phytopathol Soc Japan 62: 134–140.
- Rabindran R, Vidhyasekaran P. 1996. Development of a formulation of P. fluorescens Pf ALR 2 for management of rice sheath blight. Crop Protect. 15: 715–721.
- Vidhyasekaran P, Muthamilan M. 1995. Development of formulations of P. fluorescens for control of chickpea wilt. Plant Dis. 79: 782–786.