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Mantoo, M. A.
- Bioefficacy and mass culturing of Heterorhabditis bacteriophora (Nematoda:Heterorhabditidae) on cabbage butterfly, Pieris brassicae (Lepidoptera:Pieridae) in temperate region of Kashmir, Indi
Authors
1 Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Campus, Srinagar – 190 025, Jammu and Kashmir, IN
2 Department of Zoology, University of Kashmir, Srinagar – 190 006, Jammu and Kashmir, IN
Source
Journal of Biological Control, Vol 35, No 1 (2021), Pagination: 6-11Abstract
A laboratory investigation was carried out to assess the bioefficacy and mass culturing of Kashmir isolate of entomopathogenic nematode (EPN), Heterorhabditis bacteriophora on cabbage butterfly, Pieris brassicae in Srinagar. The pathogenicity of H. bacteriophora against 3rd, 4th and 5th larval instars of P. brassicae showed cent per cent mortality of the pest in all the three larval instars at the dosages of 20 IJs/larva and above. The LC50 values of H. bacteriophora against 3rd, 4th and 5th larval instars of P. brassicae ranged from 46.61 to 9.32, 76.33 to 9.82 and 91.36 to 10.08 IJs/larva at 24 to 96 h and that the LT50 values from 65.15 to 23.12, 84.54 to 29.20 and 105.36 to 33.52 h at 10 to 60 IJs/larva, respectively. The average production of nematode infective juveniles per larvae was found to be 7.02 x 103, 25.74 x 103 and 58.45 x 103 and that from per gram of host body weight was 91.49 x 103, 151.30 x 103 and 233.78 x 103 in 3rd, 4th and 5th larval instars of P. brassicae, respectively. The total time period between the larval mortality and the initiation of emergence of the infective juveniles from the cadavers ranged from 7-8, 9-10 and 10-11 days and the peak period of emergence of infective juveniles ranged from 10-13, 14-19, 16-21 days after mortality from the cadavers of 3rd, 4th and 5th larval instars of P. brassicae, respectively. The total duration of nematode emergence recorded during the period between larval mortality and cessation of emergence of infective juveniles from the cadavers ranged 17-19, 23-25 and 26-29 days after mortality in case of 3rd, 4th and 5th larval instars of P. brassicae, respectively.Keywords
Bioefficacy, Entomopathogenic Nematode, Heterorhabditis bacteriophora, Pieris brassicaeFull Text
References
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- In-vivo production of a Kashmir isolate of EPN, Heterorhabditis bacteriophora (SKUASTK-EPN-Hr 01) on test insect hosts from Srinagar (J&K)
Authors
1 Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Campus, Srinagar − 190025, Jammu and Kashmir, IN
2 Department of Zoology, University of Kashmir, Srinagar − 190006, Jammu and Kashmir, IN
Source
Journal of Biological Control, Vol 35, No 1 (2021), Pagination: 25-29Abstract
Under laboratory conditions, the virulence of the Kashmir isolate of EPN, Heterorhabditis bacteriophora (SKUASTK-EPN-Hr 01) to Galleria mellonella, Corcyra cephalonica and Bombyx mori showed that after 48-72 h, 100% mortality was recorded in all the test insects. Considering 48 h time and 20 IJs/larva as standard against G. mellonella, C. cephalonica and B. mori, the LC50 and LT50 values for the EPN were 10.17, 28.72 and 23.81 IJs/larva, and 38.64, 53.04 and 49.20 h after exposure, respectively. Again its virulence to the larvae of three different sizes of these insects, viz., small (0.12g, 0.04g, 0.17g), medium (0.18g, 0.05g, 0.59g) and large (0.22g, 0.07g, 2.58g) sized larvae showed that all the three sizes of G. mellonella were more susceptible than that of B. mori and C. cephalonica. Considering 20 IJs/larva as standard against small, medium and large sized larvae, the LT50 value for the EPN was 25.00, 26.45 and 27.21 h; 47.73, 48.92 and 53.16 h, and 46.04, 47.48 and 48.92 h for G. mellonella, C. cephalonica and B. mori, respectively. The production of nematode infective juveniles per larvae was directly proportional to the size and/or body weight of the insect species tested. The average production of nematode infective juveniles per gram of host body weight ranged from 1557.79 to 1933.55 x 103, 217.38 to 335.43 x 103 and 71.03 to 128.00 x 103 in G. mellonella, C. cephalonica and B. mori, respectively. The total time period between the larval mortality and the initiation of emergence, and between larval mortality and the cessation of emergence of nematode infective juveniles from the cadavers ranged from 7-10, 6-8 and 9-12 days, and 19-28, 16-24 and 23-41 days in G. mellonella, C. cephalonica and B. mori, respectively.
Keywords
Heterorhabditis bacteriophora, Bombyx mori, Corcyra cephalonica, Galleria mellonella, In-vivo Production, Virulence.Full Text
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