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Poornesha, B.

Colonization of Beauveria bassiana (Balsamo) Vuillemin Strains in Maize (Zea mays L.) and their Efficacy against Stem Borer Chilo partellus (Swinhoe)

Abstract Views :271 | PDF Views:129

Authors

S. Renuka ¹, B. Ramanujam ¹, B. Poornesha ²

Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Bellary Road, Bengaluru - 560 024, Karnataka, IN
2 ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Bellary Road, Bengaluru - 560 024, Karnataka, India, IN

Source

Journal of Biological Control, Vol 31, No 1 (2017), Pagination: 28-37

Abstract

Glasshouse experiments were conducted to study the endophytic ability of six strains of entomopathogenic fungus Beauveria bassiana in maize leaf and stem tissues when applied through foliar sprays of oil formulations. B. bassiana treated and untreated control plants were artificially infested with second instar larvae of Chilo partellus to assess the stem borer damage. All six strains showed varying percent colonization and persistence in stem and leaf tissues. The mean percent colonization ranged from 16.67-46.67 % in older stem tissues and 22.22-52.22 % in young stem tissues and in leaf, it ranged from 25.56-47.78 % in older leaf tissues and 31.11-57.78 % in young leaf tissues. Among six isolates tested, Bb-45 isolate recorded the maximum mean colonization in older stem (46.67 %), older leaf (47.78%) and in young stem (52.22 %). Bb-5a isolate showed maximum mean colonization in young leaf tissues (57.78 %). Significantly lower percentage of dead hearts (2.2-11.1%) and lower stem tunneling (2.7-4.3cm/plant) was observed in B. bassiana treated plants as compared to the untreated control plants (28.86% of dead hearts and 13.41cm/plant stem tunneling).

Keywords

Beauveria Bassiana, Biocontrol, Chilo Partellus, Endophyte, Entomopathogenic Fungus, Maize.

Full Text

References

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Field Evaluation of Entomopathogenic Fungi against Cabbage Aphid, Brevicoryne brassicae (L.) and their Effect on Coccinellid predator, Coccinella septempunctata (Linnaeus)

Abstract Views :256 | PDF Views:163

Authors

B. Ramanujam ¹, Krishna Japur ¹, B. Poornesha ¹

Affiliations
1 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources (NBAIR), H. A. Farm Post, Bellary Road, Hebbal, Bengaluru – 560024, Karnataka, IN
2 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources (NBAIR), H. A. Farm post, Bellary Road, Hebbal, Bengaluru – 560024, Karnataka, IN

Source

Journal of Biological Control, Vol 31, No 3 (2017), Pagination: 168-171

Abstract

The present study was carried out to assess the field efficacy of three isolates of entomopathogenic fungi (EPF) for management of cabbage aphid, Brevicoryne brassicae. All the tested isolates showed suppressive effect on aphid. Two-year pooled data revealed lower incidence of 13.9–17.1 aphids/plant in the EPF treated plots compared to higher incidence of 107.5 aphids/plant in untreated control. Significantly higher yields were recorded in the plots treated with Bb-5a, Ma-4 and Vl-8 isolates (28.0, 27.4 and 27.4 t/ha, respectively) compared to untreated control (22.3 t/ha). The populations of predatory coccinellid and spiders in EPF treated and untreated control plots were found statistically non-significant indicating the safety of entomopathogenic fungi to these predators.

Keywords

Beauveria bassiana, Brevicoryne brassicae, Cabbage, Coccinella septempunctata, Entomopathogenic Fungi, Lecanicillium lecanii, Metarhizium anisopliae.

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Establishment of Metarhizium anisopliae (Metchnikoff) Sorokin as Endophyte in Maize and Sorghum

Abstract Views :282 | PDF Views:141

Authors

B. Ramanujam ¹, B. Poornesha ¹

Affiliations
1 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources, H. A. Farm post, Bellary Road, Hebbal, Bengaluru - 560024, Karnataka, IN

Source

Journal of Biological Control, Vol 32, No 3 (2018), Pagination: 165-171

Abstract

Glasshouse experiments were conducted to establish an indigenous soil-derived strain of Metarhizium anisopliae (ICAR-NBAIR Ma-35) as endophyte in maize and sorghum by artificial inoculation through foliar spray of conidial suspension. Colonization of M. anisopliae were studied at 15, 30, 45, 60, 75 and 90 Days After Treatment (DAT) in stem and leaf tissues of maize and sorghum by plating technique and PCR method. Metarhizium anisopliae showed variation with regard to the extent of colonization in stem and leaf tissues of maize and sorghum. In maize, colonization was observed at 30DAT in stem and 30-45DAT in leaf. In sorghum, colonization of M. anisopliae was observed during 15-60DAT in stem and 15-75DAT in leaf indicating longer persistence compared to maize. Colonization of M. anisopliae was observed in young growing stem tips and leaves (unsprayed) indicating the internal spread of M. anisopliae in maize and sorghum plants. M. anisopliae was not detected in the untreated stem and leaf tissues of maize and sorghum. The positive results of colonization of M. anisopliae in maize and sorghum stem/leaf tissues observed in plating technique were confirmed by the PCR amplification.

Keywords

Endophyte, Entomopathogenic Fungi, Maize, Metarhizium anisopliae, Sorghum.

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Natural Occurrence of Entomopathogenic Fungus, Cladosporium cladosporioides on Blow Fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) on Ber in Punjab, India

Abstract Views :245 | PDF Views:110

Authors

Sandeep Singh ¹, B. Poornesha ², Rajwinder Kaur Sandhu ¹, Boman Ramanujam ²

Affiliations
1 Department of Fruit Science, Punjab Agricultural University, Ludhiana − 141004, Punjab, IN
2 ICAR-National Bureau of Agricultural Insect Resources, Bengaluru − 560024, Karnataka, IN

Source

Journal of Biological Control, Vol 34, No 2 (2020), Pagination: 140-143

Abstract

Surveys and surveillances were carried out in the ber growing regions of Punjab through fixed plot surveys in Fruit Research Farm, Punjab Agricultural University (PAU), Ludhiana, Punjab during 2018 and 2019 to record the emerging insect pests and their natural enemies. During these surveys, infection of a fungus was observed on the adults of blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) occurring on ber plants in PAU, Ludhiana. This fly is economically significant as pollinator of fruit crops. The adult flies were observed to be dead and were covered with fungal mycelial growth. Later on, the fungus was identified as an entomopathogenic fungus, Cladosporium cladosporioides based on morphological and molecular characterization. This is the first record of infection of an entomopathogenic fungus, C. cladosporioides on this pollinator dipteran fly, C. megacephala from Punjab, India. Thus, the potential of C. cladosporioides for the control of other dipteran insect-pests of fruit crops can be explored.

Keywords

Ber, Biocontrol, Blow Fly, Cladosporium cladosporioides, Entomopathogenic Fungus, Pollinator.

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Natural occurrence of entomopathogenic fungus, Aschersonia aleyrodis on citrus whitefly, Dialeurodes citri (Ashmead) in Kinnow mandarin in Punjab, India

Abstract Views :182 | PDF Views:93

Authors

Sandeep Singh ¹, Bonam Ramanujam ², Rajwinder Kaur Sandhu ², A. Kandan ², B. Poornesha ², Rupa Kundu ²

Affiliations
1 Department of Fruit Science, Punjab Agricultural University, Ludhiana – 141004, Punjab, IN
2 ICAR-National Bureau of Agricultural Insect Resources (NBAIR), Bengaluru – 560024, Karnataka, IN

Source

Journal of Biological Control, Vol 35, No 3 (2021), Pagination: 181-186

Abstract

Surveys were conducted during 2017 and 2018 in the citrus orchards of Punjab, India to record the incidence of different insect pests and their natural enemies. During October-December, Entomopathogenic Fungus (EPF), Aschersonia aleyrodis was found to infect nymphs and pupae of citrus whitefly, Dialeurodes citri on the lower leaf surface of Kinnow from the orchards of Hoshiarpur, Ludhiana, Mansa and Fazilka districts.The fungus was isolated from the infected nymphs and pupae and morphological studies were conducted to confirm the identity of the entomopathogenic fungus. Aschersonia aleyrodis was reported for the first time on D. citri under Punjab conditions and this EPF also confirmed by amplification and sequencing of beta tubulin gene showed 99.40 per cent identity in NCBI, GenBank. Hence further studies on the host range, interaction with other insect pests and parasitoids, survival and longevity should be conducted to explore the potential of this fungus as microbial biocontrol agent for citrus whitefly.

Keywords

Aschersonia aleyrodis, biological control, citrus whitefly, entomopathogenic fungi, Punjab

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