A. K. Pandey ¹

Affiliations
1 College of Forestry & Hill Agriculture, G. B. Pant University of Agriculture & Technology, Hill Campus, Ranichauri, Tehri, Garhwal, 249 199, Uttarakhand, IN

Abstract

Field experiments were conducted at Crop Research Center, College of Forestry and Hill Agriculture, GBPUA&T, Ranichauri during 2006-2009 to evaluate the effectiveness of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae with few insecticides against white grub, Holotrichia longipennis damaging soybean. On the basis of average cumulative plant mortality during the study, M. anisopliae (5.0×10¹³ spores g^-1) was found to be superior compared to other bio-agents by recording lowest plant mortality (21.83%) and 61.58% decrease in grub population followed by B. bassiana (5.0×10¹³ spores g^-1) where the plant mortality and decrease in grub population was 25.18% and 54.75%, respectively, compared to control. Highest percentage of yield increase (113.41%) was also recorded with M. anisopliae. However, among all the treatments, imidacloprid was found to be the most effective in reducing the grub population (82.28%) and thereby increasing the yield 206.86% over control.

Keywords

Beauveria, Metarhizium, Imidacloprid, Chlorpyriphos, Bio-Efficacy, Holotrichia longipennis, Soybean.

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A. K. Pandey ¹, K. R. Kanaujia ²

Affiliations
1 College of Forestry & Hill Agriculture, G B. Pant Univ. of Ag. & Tech. Hill Campus, Ranichauri, Tehri Garhwal - 249199 (Uttarakhand), IN
2 Department of Entomology, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, IN

Abstract

Influence of different cereal grains as solid substrates on sporulation, viability and pathogenicity of Metarhizium anisopliae (Metschnikoff) Sorokin was studied under laboratory condition. Among the six grains (viz. maize, sorghum, fingermillet, sorghum, barley and wheat), sorghum as solid substrate resulted in highest spore (6.36 × 10⁷ conidia ml⁻¹) production with spore viability of 86.6 per cent. The production and viability of conidia increased when the substrates were supplemented with sucrose as compared to conidia produced from substrates alone. Conidia produced from fingermillet showed highest virulence against third instar larvae of Spodoptera litura with LC₅₀, value of 1,72 × 10⁶ conidia ml⁻¹ compared to conidia from other sources. However, conidia produced from SDA medium showed highest spore production, viability and virulence to S. litura larvae compared to conidia produced from solid substrates.

Keywords

Metarhizium, Pathogenicity, Solid Substrates Fermentation, Spodoptera Sporulation, Viability.

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S. Farkya ¹, A. K. Pandey ¹, R. C. Rajak ¹

Affiliations
1 Department of Biological Science Rani Durgawati University Jabalpur 482001, M. P., IN

Abstract

Factors affecting mycoherbicidal potential of two indigenous, Fusarium spp. viz. F. oxysporum PR # 12 and F. solani PR # 13 inciting seedling blight and wilt in plants of Parthenium hysterophorus L. were investigated. In preliminary green house experiments, both the pathogens favoured sandy loam soil. 25°C±1°C temperature, 70 per cent soil moisture and 100 per cent relative humidity for maximum seedling mortality. Soil pH 6.0 and 7.0 were favourable for maximum control of this weed by F. oxysporum PR # 12 and F. solani PR # 13, respectively.

Keywords

Fusarium oxysporum, F. solani, Mycoherbicidal, Parthenium.

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